Abstract
An off-line optimization technique based on soft modeling methods has been used to determine the optimum conditions for the separation of eight carbonyl-2,4-dinitrophenylhydrazones by gas chromatography equipped with a nitrogen-phosphorous detector. In this method theoretical simulations of peak retention time and peak width at base-line are coupled to a factorial design to map the chromatographic response surface. The calculated retention times from isothermal data have been used to calculate the chromatographic parameters. A full 33 factorial design was used to locate the optimum conditions for the temperature program, utilizing initial oven temperature, Ti, the programming rate for the first ramp, PR1, and the programming rate for the second ramp, PR2 as independent variables. The optimum conditions obtained for these parameters were: Ti, 140 °C; PR1, 12 °C min-1; PR2, 8 °C min -1. The maximum analysis times achieved by this optimum was 10.6 min.
| Original language | English |
|---|---|
| Pages (from-to) | 175-183 |
| Number of pages | 9 |
| Journal | Talanta |
| Volume | 56 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 4 2002 |
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Keywords
- 2,4-Dinitrophenylhydrazones
- Experimental design
- Gas chromatography
- Optimization
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
Cite this
Off-line optimization of the separation of 2,4-dinitrophenylhydrazones by gas chromatography using chemometric techniques. / Suliman, F. E O.
In: Talanta, Vol. 56, No. 1, 04.01.2002, p. 175-183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Off-line optimization of the separation of 2,4-dinitrophenylhydrazones by gas chromatography using chemometric techniques
AU - Suliman, F. E O
PY - 2002/1/4
Y1 - 2002/1/4
N2 - An off-line optimization technique based on soft modeling methods has been used to determine the optimum conditions for the separation of eight carbonyl-2,4-dinitrophenylhydrazones by gas chromatography equipped with a nitrogen-phosphorous detector. In this method theoretical simulations of peak retention time and peak width at base-line are coupled to a factorial design to map the chromatographic response surface. The calculated retention times from isothermal data have been used to calculate the chromatographic parameters. A full 33 factorial design was used to locate the optimum conditions for the temperature program, utilizing initial oven temperature, Ti, the programming rate for the first ramp, PR1, and the programming rate for the second ramp, PR2 as independent variables. The optimum conditions obtained for these parameters were: Ti, 140 °C; PR1, 12 °C min-1; PR2, 8 °C min -1. The maximum analysis times achieved by this optimum was 10.6 min.
AB - An off-line optimization technique based on soft modeling methods has been used to determine the optimum conditions for the separation of eight carbonyl-2,4-dinitrophenylhydrazones by gas chromatography equipped with a nitrogen-phosphorous detector. In this method theoretical simulations of peak retention time and peak width at base-line are coupled to a factorial design to map the chromatographic response surface. The calculated retention times from isothermal data have been used to calculate the chromatographic parameters. A full 33 factorial design was used to locate the optimum conditions for the temperature program, utilizing initial oven temperature, Ti, the programming rate for the first ramp, PR1, and the programming rate for the second ramp, PR2 as independent variables. The optimum conditions obtained for these parameters were: Ti, 140 °C; PR1, 12 °C min-1; PR2, 8 °C min -1. The maximum analysis times achieved by this optimum was 10.6 min.
KW - 2,4-Dinitrophenylhydrazones
KW - Experimental design
KW - Gas chromatography
KW - Optimization
UR - http://www.scopus.com/inward/record.url?scp=0037016318&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037016318&partnerID=8YFLogxK
U2 - 10.1016/S0039-9140(01)00554-9
DO - 10.1016/S0039-9140(01)00554-9
M3 - Article
VL - 56
SP - 175
EP - 183
JO - Talanta
JF - Talanta
SN - 0039-9140
IS - 1
ER -