Nuclear factor-kappa B as a promising target for selenium chemoprevention in rat hepatocarcinogenesis

Nasar Y. Alwahaibi, Siti B. Budin, Jamaludin Mohamed, Aisha Alhamdani

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background and Aims: Selenium's molecular mechanism for cancer chemoprevention remains unknown. We aimed to study the gene expression of nuclear factor-κB (NF-κB), tumor growth factor-α (TGF-α) and cyclin D1 and the effects of sodium selenite using preventive and therapeutic approaches in chemically-induced hepatocarcinogenesis in rats. Methods: Rats were divided randomly into six groups: negative control, positive control (diethyl nitrosamine [DEN] + 2-acetylaminofluorene [2-AAF]), preventive group, preventive control (respective control for preventive group), therapeutic group and therapeutic control (respective control for therapeutic group). The relative gene expression of NF-κB, TGF-α and cyclin D1 in liver tissues were measured using real-time polymerase chain reaction. Results: The findings showed that the gene expression of NF-κB in the preventive group and its respective control was significantly lower (P <0.05) when compared with both the negative and positive controls. However, the expression of NF-κB in the positive controls and therapeutic group was significantly higher (P <0.05) when compared with the negative controls. The expression of TGF-α and cyclin D1 was insignificant in all groups. Conclusion: The inhibition of the NF-κB pathway in the initiation phase of hepatocarcinogenesis could be a promising target for selenium chemoprevention. However, further studies are required.

Original languageEnglish
Pages (from-to)786-791
Number of pages6
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume25
Issue number4
DOIs
Publication statusPublished - 2010

Keywords

  • Cyclin D1
  • Hepatocarcinogenesis
  • Nuclear factor-κB
  • Selenium
  • Transforming growth factor-α

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

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