TY - JOUR
T1 - Nuclear factor-kappa B as a promising target for selenium chemoprevention in rat hepatocarcinogenesis
AU - Alwahaibi, Nasar Y.
AU - Budin, Siti B.
AU - Mohamed, Jamaludin
AU - Alhamdani, Aisha
PY - 2010/4
Y1 - 2010/4
N2 - Background and Aims: Selenium's molecular mechanism for cancer chemoprevention remains unknown. We aimed to study the gene expression of nuclear factor-κB (NF-κB), tumor growth factor-α (TGF-α) and cyclin D1 and the effects of sodium selenite using preventive and therapeutic approaches in chemically-induced hepatocarcinogenesis in rats. Methods: Rats were divided randomly into six groups: negative control, positive control (diethyl nitrosamine [DEN] + 2-acetylaminofluorene [2-AAF]), preventive group, preventive control (respective control for preventive group), therapeutic group and therapeutic control (respective control for therapeutic group). The relative gene expression of NF-κB, TGF-α and cyclin D1 in liver tissues were measured using real-time polymerase chain reaction. Results: The findings showed that the gene expression of NF-κB in the preventive group and its respective control was significantly lower (P < 0.05) when compared with both the negative and positive controls. However, the expression of NF-κB in the positive controls and therapeutic group was significantly higher (P < 0.05) when compared with the negative controls. The expression of TGF-α and cyclin D1 was insignificant in all groups. Conclusion: The inhibition of the NF-κB pathway in the initiation phase of hepatocarcinogenesis could be a promising target for selenium chemoprevention. However, further studies are required.
AB - Background and Aims: Selenium's molecular mechanism for cancer chemoprevention remains unknown. We aimed to study the gene expression of nuclear factor-κB (NF-κB), tumor growth factor-α (TGF-α) and cyclin D1 and the effects of sodium selenite using preventive and therapeutic approaches in chemically-induced hepatocarcinogenesis in rats. Methods: Rats were divided randomly into six groups: negative control, positive control (diethyl nitrosamine [DEN] + 2-acetylaminofluorene [2-AAF]), preventive group, preventive control (respective control for preventive group), therapeutic group and therapeutic control (respective control for therapeutic group). The relative gene expression of NF-κB, TGF-α and cyclin D1 in liver tissues were measured using real-time polymerase chain reaction. Results: The findings showed that the gene expression of NF-κB in the preventive group and its respective control was significantly lower (P < 0.05) when compared with both the negative and positive controls. However, the expression of NF-κB in the positive controls and therapeutic group was significantly higher (P < 0.05) when compared with the negative controls. The expression of TGF-α and cyclin D1 was insignificant in all groups. Conclusion: The inhibition of the NF-κB pathway in the initiation phase of hepatocarcinogenesis could be a promising target for selenium chemoprevention. However, further studies are required.
KW - Cyclin D1
KW - Hepatocarcinogenesis
KW - Nuclear factor-κB
KW - Selenium
KW - Transforming growth factor-α
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U2 - 10.1111/j.1440-1746.2009.06160.x
DO - 10.1111/j.1440-1746.2009.06160.x
M3 - Article
C2 - 20492335
AN - SCOPUS:77950131994
SN - 0815-9319
VL - 25
SP - 786
EP - 791
JO - Journal of Gastroenterology and Hepatology (Australia)
JF - Journal of Gastroenterology and Hepatology (Australia)
IS - 4
ER -