Novel Polymorphic Microsatellite Markers for Panulirus ornatus and their Cross-species Primer Amplification in Panulirus homarus

Madjid Delghandi, Hasifa Afzal, Manal Saif Nasser Al Hinai, Rafaida Dhuhai Gharib Al-Breiki, Dean R. Jerry, Hoc Tan Dao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Polymorphic microsatellite loci were isolated for Panulirus ornatus using 454 GS-FLX Titanium pyrosequencing. Fifteen markers containing perfect di-, tri-, tetra-, and penta-nucleotide motifs were consistently co-amplified in five multiplexes in a panel of 91 randomly selected samples. Observed number of alleles varied from 2 to 14 per locus. Observed and expected heterozygosity ranged from 0.090 to 0.79 and 0.08 to 0.87, respectively. Ten loci deviated from Hardy-Weinberg equilibrium after sequential Bonferroni correction. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between 11 loci. The microsatellite markers were also amplified successfully in related Panulirus homarus species with adequate level of polymorphism. The successful cross-species primer amplification of the 15 microsatellites indicates the potential of the developed markers to be transferred to other Panulirus species. The 15 novel microsatellite markers reported in this work add to the previously characterized markers by our group, exhibit adequate levels of polymorphism for wide range of future studies investigating population structure, genetic diversity, and evolutionary relationships among Panulirus species.

Original languageEnglish
Pages (from-to)310-314
Number of pages5
JournalAnimal Biotechnology
Volume27
Issue number4
DOIs
Publication statusPublished - Oct 1 2016

Keywords

  • 454 sequencing
  • microsatellites
  • multiplexing
  • panulirus
  • spiny lobster

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine(all)
  • Animal Science and Zoology

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