Novel flavivirus or new lineage of West Nile virus, Central Europe

Tamás Bakonyi; Zdenek Hubálek; Ivo Rudolf; Norbert Nowotny

doi:10.3201/eid1102.041028

Novel flavivirus or new lineage of West Nile virus, Central Europe

Tamás Bakonyi, Zdenek Hubálek, Ivo Rudolf, Norbert Nowotny^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

192 Citations (Scopus)

Abstract

A flavivirus (strain 97-103) was isolated from Culex pipens mosquitoes in 1997 following floods in South Moravia, Czech Republic. The strain exhibited close antigenic relationship to West Nile virus (WNV) prototype strain Eg-101 in a cross-neutralization test. In this study, mouse pathogenicity characteristics and the complete nucleotide and putative amino acid sequences of isolate 97-103, named Rabensburg virus (RabV) after a nearby Austrian city, were determined. RabV shares only 75%-77% nucleotide identity and 89%-90% amino acid identity with representative strains of WNV lineages 1 and 2. Another RabV strain (99-222) was isolated in the same location 2 years later; it showed >99% nucleotide identity to strain 97-103. Phylogenetic analyses of RabV, WNV strains, and other members of the Japanese encephalitis virus (JEV) complex clearly demonstrated that RabV is either a new (third) lineage of WNV or a novel flavivirus of the JEV group.

Original language	English
Pages (from-to)	225-231
Number of pages	7
Journal	Emerging Infectious Diseases
Volume	11
Issue number	2
DOIs	https://doi.org/10.3201/eid1102.041028
Publication status	Published - Feb 2005
Externally published	Yes

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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abstract = "A flavivirus (strain 97-103) was isolated from Culex pipens mosquitoes in 1997 following floods in South Moravia, Czech Republic. The strain exhibited close antigenic relationship to West Nile virus (WNV) prototype strain Eg-101 in a cross-neutralization test. In this study, mouse pathogenicity characteristics and the complete nucleotide and putative amino acid sequences of isolate 97-103, named Rabensburg virus (RabV) after a nearby Austrian city, were determined. RabV shares only 75%-77% nucleotide identity and 89%-90% amino acid identity with representative strains of WNV lineages 1 and 2. Another RabV strain (99-222) was isolated in the same location 2 years later; it showed >99% nucleotide identity to strain 97-103. Phylogenetic analyses of RabV, WNV strains, and other members of the Japanese encephalitis virus (JEV) complex clearly demonstrated that RabV is either a new (third) lineage of WNV or a novel flavivirus of the JEV group.",

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AU - Hubálek, Zdenek

AU - Rudolf, Ivo

AU - Nowotny, Norbert

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Novel flavivirus or new lineage of West Nile virus, Central Europe

Abstract

ASJC Scopus subject areas

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