Nose-only water-pipe smoking effects on airway resistance, inflammation, and oxidative stress in mice

Abderrahim Nemmar, Haider Raza, Priya Yuvaraju, Sumaya Beegam, Annie John, Javed Yasin, Rasheed S. Hameed, Ernest Adeghate, Badreldin H. Ali

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Water-pipe smoking (WPS) is a common practice in the Middle East and is now gaining popularity in Europe and the United States. However, there is a limited number of studies on the respiratory effects of WPS. More specifically, the underlying pulmonary pathophysiological mechanisms related to WPS exposure are not understood. Presently, we assessed the respiratory effects of nose-only exposure to mainstream WPS generated by commercially available honey flavored "moasel" tobacco. The duration of the session was 30 min/day and 5 days/wk for 1 mo. Control mice were exposed to air only. Here, we measured in BALB/c mice the airway resistance using forced-oscillation technique. Lung inflammation was assessed histopathologically and by biochemical analysis of bronchoalveolar lavage (BAL) fluid, and oxidative stress was evaluated biochemically by measuring lipid peroxidation, reduced glutathione and several antioxidant enzymes. Pulmonary inflammation assessment showed an increase in neutrophil and lymphocyte numbers. Likewise, airway resistance was significantly increased in the WPS group compared with controls. Tumor necrosis factor α and interleukin 6 concentrations were significantly increased in BAL fluid. Lipid peroxidation in lung tissue was significantly increased whereas the level and activity of antioxidants including reduced glutathione, glutathione S transferase, and superoxide dismutase were all significantly decreased following WPS exposure, indicating the occurrence of oxidative stress. Moreover, carboxyhemoglobin levels were significantly increased in the WPS group. We conclude that 1-mo nose-only exposure to WPS significantly increased airway resistance, inflammation, and oxidative stress. Our results provide a mechanistic explanation for the limited clinical studies that reported the detrimental respiratory effects of WPS.

Original languageEnglish
Pages (from-to)1316-1323
Number of pages8
JournalJournal of Applied Physiology
Volume115
Issue number9
DOIs
Publication statusPublished - Nov 1 2013

Fingerprint

Airway Resistance
Nose
Oxidative Stress
Smoking
Inflammation
Water
Bronchoalveolar Lavage Fluid
Lipid Peroxidation
Glutathione
Pneumonia
Antioxidants
Carboxyhemoglobin
Lung
Middle East
Honey
Lymphocyte Count
Glutathione Transferase
Superoxide Dismutase
Tobacco
Interleukin-6

Keywords

  • Airway resistance
  • Inflammation
  • Nose-only exposure
  • Oxidative stress
  • Water-pipe smoking

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Nose-only water-pipe smoking effects on airway resistance, inflammation, and oxidative stress in mice. / Nemmar, Abderrahim; Raza, Haider; Yuvaraju, Priya; Beegam, Sumaya; John, Annie; Yasin, Javed; Hameed, Rasheed S.; Adeghate, Ernest; Ali, Badreldin H.

In: Journal of Applied Physiology, Vol. 115, No. 9, 01.11.2013, p. 1316-1323.

Research output: Contribution to journalArticle

Nemmar, A, Raza, H, Yuvaraju, P, Beegam, S, John, A, Yasin, J, Hameed, RS, Adeghate, E & Ali, BH 2013, 'Nose-only water-pipe smoking effects on airway resistance, inflammation, and oxidative stress in mice', Journal of Applied Physiology, vol. 115, no. 9, pp. 1316-1323. https://doi.org/10.1152/japplphysiol.00194.2013
Nemmar, Abderrahim ; Raza, Haider ; Yuvaraju, Priya ; Beegam, Sumaya ; John, Annie ; Yasin, Javed ; Hameed, Rasheed S. ; Adeghate, Ernest ; Ali, Badreldin H. / Nose-only water-pipe smoking effects on airway resistance, inflammation, and oxidative stress in mice. In: Journal of Applied Physiology. 2013 ; Vol. 115, No. 9. pp. 1316-1323.
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