NMR-based metabolomics for organic farming traceability of early potatoes

Daniela Pacifico, Lorena Casciani, Mena Ritota, Giuseppe Mandolino, Chiara Onofri, Anna Moschella, Bruno Parisi, Caterina Cafiero, Massimiliano Valentini

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

1H HRMAS-NMR spectroscopy was successfully used to determine the metabolic profiles of 78 tubers obtained from three early genotypes grown under organic and conventional management. The variation in total hydrogen, carbon, and nitrogen contents was also assessed. A PLS-DA multivariate statistical analysis provided good discrimination among the varieties and cropping systems (100% unknown samples placed in a cross-validation blind test), suggesting that this method is a powerful and rapid tool for tracing organic potatoes. As a result of the farming system, the nitrogen content decreased by 11-14% in organic tubers, whereas GABA and lysine accumulated in the organic tubers of all clones. Clear variations in primary metabolites are discussed to provide a better understanding of the metabolic pathway modifications resulting from agronomical practices.

Original languageEnglish
Pages (from-to)11201-11211
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number46
DOIs
Publication statusPublished - Nov 20 2013

Fingerprint

Organic Agriculture
Metabolomics
traceability
metabolomics
Solanum tuberosum
organic production
tubers
Nitrogen
Nuclear magnetic resonance
potatoes
nitrogen content
Metabolome
Metabolites
Metabolic Networks and Pathways
Agriculture
gamma-Aminobutyric Acid
Nuclear magnetic resonance spectroscopy
Lysine
Hydrogen
Statistical methods

Keywords

  • CHN
  • HRMAS-NMR
  • PLS-DA
  • Solanum tuberosum L.

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Pacifico, D., Casciani, L., Ritota, M., Mandolino, G., Onofri, C., Moschella, A., ... Valentini, M. (2013). NMR-based metabolomics for organic farming traceability of early potatoes. Journal of Agricultural and Food Chemistry, 61(46), 11201-11211. https://doi.org/10.1021/jf402961m

NMR-based metabolomics for organic farming traceability of early potatoes. / Pacifico, Daniela; Casciani, Lorena; Ritota, Mena; Mandolino, Giuseppe; Onofri, Chiara; Moschella, Anna; Parisi, Bruno; Cafiero, Caterina; Valentini, Massimiliano.

In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 46, 20.11.2013, p. 11201-11211.

Research output: Contribution to journalArticle

Pacifico, D, Casciani, L, Ritota, M, Mandolino, G, Onofri, C, Moschella, A, Parisi, B, Cafiero, C & Valentini, M 2013, 'NMR-based metabolomics for organic farming traceability of early potatoes', Journal of Agricultural and Food Chemistry, vol. 61, no. 46, pp. 11201-11211. https://doi.org/10.1021/jf402961m
Pacifico D, Casciani L, Ritota M, Mandolino G, Onofri C, Moschella A et al. NMR-based metabolomics for organic farming traceability of early potatoes. Journal of Agricultural and Food Chemistry. 2013 Nov 20;61(46):11201-11211. https://doi.org/10.1021/jf402961m
Pacifico, Daniela ; Casciani, Lorena ; Ritota, Mena ; Mandolino, Giuseppe ; Onofri, Chiara ; Moschella, Anna ; Parisi, Bruno ; Cafiero, Caterina ; Valentini, Massimiliano. / NMR-based metabolomics for organic farming traceability of early potatoes. In: Journal of Agricultural and Food Chemistry. 2013 ; Vol. 61, No. 46. pp. 11201-11211.
@article{0958461b1bfb452f88e6a48a4a3c08f7,
title = "NMR-based metabolomics for organic farming traceability of early potatoes",
abstract = "1H HRMAS-NMR spectroscopy was successfully used to determine the metabolic profiles of 78 tubers obtained from three early genotypes grown under organic and conventional management. The variation in total hydrogen, carbon, and nitrogen contents was also assessed. A PLS-DA multivariate statistical analysis provided good discrimination among the varieties and cropping systems (100{\%} unknown samples placed in a cross-validation blind test), suggesting that this method is a powerful and rapid tool for tracing organic potatoes. As a result of the farming system, the nitrogen content decreased by 11-14{\%} in organic tubers, whereas GABA and lysine accumulated in the organic tubers of all clones. Clear variations in primary metabolites are discussed to provide a better understanding of the metabolic pathway modifications resulting from agronomical practices.",
keywords = "CHN, HRMAS-NMR, PLS-DA, Solanum tuberosum L.",
author = "Daniela Pacifico and Lorena Casciani and Mena Ritota and Giuseppe Mandolino and Chiara Onofri and Anna Moschella and Bruno Parisi and Caterina Cafiero and Massimiliano Valentini",
year = "2013",
month = "11",
day = "20",
doi = "10.1021/jf402961m",
language = "English",
volume = "61",
pages = "11201--11211",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "46",

}

TY - JOUR

T1 - NMR-based metabolomics for organic farming traceability of early potatoes

AU - Pacifico, Daniela

AU - Casciani, Lorena

AU - Ritota, Mena

AU - Mandolino, Giuseppe

AU - Onofri, Chiara

AU - Moschella, Anna

AU - Parisi, Bruno

AU - Cafiero, Caterina

AU - Valentini, Massimiliano

PY - 2013/11/20

Y1 - 2013/11/20

N2 - 1H HRMAS-NMR spectroscopy was successfully used to determine the metabolic profiles of 78 tubers obtained from three early genotypes grown under organic and conventional management. The variation in total hydrogen, carbon, and nitrogen contents was also assessed. A PLS-DA multivariate statistical analysis provided good discrimination among the varieties and cropping systems (100% unknown samples placed in a cross-validation blind test), suggesting that this method is a powerful and rapid tool for tracing organic potatoes. As a result of the farming system, the nitrogen content decreased by 11-14% in organic tubers, whereas GABA and lysine accumulated in the organic tubers of all clones. Clear variations in primary metabolites are discussed to provide a better understanding of the metabolic pathway modifications resulting from agronomical practices.

AB - 1H HRMAS-NMR spectroscopy was successfully used to determine the metabolic profiles of 78 tubers obtained from three early genotypes grown under organic and conventional management. The variation in total hydrogen, carbon, and nitrogen contents was also assessed. A PLS-DA multivariate statistical analysis provided good discrimination among the varieties and cropping systems (100% unknown samples placed in a cross-validation blind test), suggesting that this method is a powerful and rapid tool for tracing organic potatoes. As a result of the farming system, the nitrogen content decreased by 11-14% in organic tubers, whereas GABA and lysine accumulated in the organic tubers of all clones. Clear variations in primary metabolites are discussed to provide a better understanding of the metabolic pathway modifications resulting from agronomical practices.

KW - CHN

KW - HRMAS-NMR

KW - PLS-DA

KW - Solanum tuberosum L.

UR - http://www.scopus.com/inward/record.url?scp=84888386457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888386457&partnerID=8YFLogxK

U2 - 10.1021/jf402961m

DO - 10.1021/jf402961m

M3 - Article

C2 - 24191752

AN - SCOPUS:84888386457

VL - 61

SP - 11201

EP - 11211

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 46

ER -