New spectrofluorimetric method for determination of cephalosporins in pharmaceutical formulations

Shazalia M. Ali Ahmed, Abdalla A. Elbashir, Fakhr Eldin O Suliman, Hassan Y. Aboul-Enein

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple, accurate, precise spectrofluorimetric method has been proposed for the determination of three cephalosporins, namely, cefixime (cefi), cephalexine (ceph), and cefotaxime sodium (cefo) in pharmaceutical formulations. This method is based on a reaction between cephalosporins with 8-hydroxy-1,3,6-pyrenetrisulfonic acid trisodium salt (HPTS) in alkaline medium, at pH 12.0 for cefi and 13.0 for ceph and cefo to give highly fluorescent derivatives extracted with chloroform and subsequent measurements of the formed fluorescent products at 520, 500 and 510 nm after excitation at 480, 470 and 480 nm for cefi, ceph and cefo respectively. The optimum experimental conditions have been studied. Beer's law is obeyed over concentrations of 10-60 ng/mL, 5-35 ng/mL and 10-60 ng/mL for cefi, ceph and cefo, respectively. The detection limits were 4.20 ng/mL, 2.54 ng/mL and 4.09 ng/mL for cefi, ceph and cefo, respectively, with a linear regression correlation coefficient of 0.99783, 0.99705 and 0.9978 and recoveries in ranges 96.96-105.77, 96.13-102.55 and 95.45-105.39% for cefi, ceph and cefo, respectively. This method is simple and can be applied for the determination of cefi, ceph and cefo in pharmaceutical formulations in quality control laboratories.

Original languageEnglish
Pages (from-to)734-741
Number of pages8
JournalLuminescence
Volume28
Issue number5
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Cefixime
Cephalexin
Drug Compounding
Cefotaxime
Cephalosporins
Pharmaceutical Preparations
Chloroform
Linear regression
Quality Control
Quality control
Limit of Detection
Linear Models
Derivatives
Recovery

Keywords

  • 8-hydroxy-1,3,6-pyrenetrisulfonic acid trisodium salt (HPTS)
  • cephalosporins
  • pharmaceutical analysis
  • spectrofluorimetric analysis

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)
  • Medicine(all)

Cite this

New spectrofluorimetric method for determination of cephalosporins in pharmaceutical formulations. / Ali Ahmed, Shazalia M.; Elbashir, Abdalla A.; Suliman, Fakhr Eldin O; Aboul-Enein, Hassan Y.

In: Luminescence, Vol. 28, No. 5, 09.2013, p. 734-741.

Research output: Contribution to journalArticle

Ali Ahmed, Shazalia M. ; Elbashir, Abdalla A. ; Suliman, Fakhr Eldin O ; Aboul-Enein, Hassan Y. / New spectrofluorimetric method for determination of cephalosporins in pharmaceutical formulations. In: Luminescence. 2013 ; Vol. 28, No. 5. pp. 734-741.
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