New polymorphic di-nucleotide microsatellite markers for Atlantic cod (Gadus morhua L.)

Madjid Delghandi, Mette Serine Wesmajervi, Saskia Mennen, Frank Nilsen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96 unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.

Original languageEnglish
Pages (from-to)1037-1040
Number of pages4
JournalConservation Genetics
Volume10
Issue number4
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Gadus morhua
Microsatellite Repeats
Nucleotides
nucleotides
Expressed Sequence Tags
microsatellite repeats
loci
Nucleotide Motifs
expressed sequence tags
Genetic Linkage
Linkage Disequilibrium
Population Genetics
Ecology
Fishes
Alleles
disequilibrium
heterozygosity
population genetics
allele
linkage disequilibrium

Keywords

  • Atlantic cod
  • cDNA
  • Expressed sequence tags (eSTs)
  • Gadus morhua
  • Microsatellites
  • Type I markers

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

New polymorphic di-nucleotide microsatellite markers for Atlantic cod (Gadus morhua L.). / Delghandi, Madjid; Wesmajervi, Mette Serine; Mennen, Saskia; Nilsen, Frank.

In: Conservation Genetics, Vol. 10, No. 4, 08.2009, p. 1037-1040.

Research output: Contribution to journalArticle

Delghandi, Madjid ; Wesmajervi, Mette Serine ; Mennen, Saskia ; Nilsen, Frank. / New polymorphic di-nucleotide microsatellite markers for Atlantic cod (Gadus morhua L.). In: Conservation Genetics. 2009 ; Vol. 10, No. 4. pp. 1037-1040.
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