TY - JOUR
T1 - New polymorphic di-nucleotide microsatellite markers for Atlantic cod (Gadus morhua L.)
AU - Delghandi, Madjid
AU - Wesmajervi, Mette Serine
AU - Mennen, Saskia
AU - Nilsen, Frank
N1 - Funding Information:
Acknowledgements The present study was supported by the National Programme for Research in Functional Genomics in Norway (FUGE) within the Research Council of Norway (GenoFish). We thank Dr. Marie Cooper for reading of the manuscript and making helpful suggestions.
PY - 2009/8
Y1 - 2009/8
N2 - Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96 unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
AB - Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96 unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
KW - Atlantic cod
KW - Expressed sequence tags (eSTs)
KW - Gadus morhua
KW - Microsatellites
KW - Type I markers
KW - cDNA
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U2 - 10.1007/s10592-008-9682-2
DO - 10.1007/s10592-008-9682-2
M3 - Article
AN - SCOPUS:67649363858
VL - 10
SP - 1037
EP - 1040
JO - Conservation Genetics
JF - Conservation Genetics
SN - 1566-0621
IS - 4
ER -