Abstract
Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96 unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
| Original language | English |
|---|---|
| Pages (from-to) | 1037-1040 |
| Number of pages | 4 |
| Journal | Conservation Genetics |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 2009 |
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Keywords
- Atlantic cod
- cDNA
- Expressed sequence tags (eSTs)
- Gadus morhua
- Microsatellites
- Type I markers
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
Cite this
New polymorphic di-nucleotide microsatellite markers for Atlantic cod (Gadus morhua L.). / Delghandi, Madjid; Wesmajervi, Mette Serine; Mennen, Saskia; Nilsen, Frank.
In: Conservation Genetics, Vol. 10, No. 4, 08.2009, p. 1037-1040.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - New polymorphic di-nucleotide microsatellite markers for Atlantic cod (Gadus morhua L.)
AU - Delghandi, Madjid
AU - Wesmajervi, Mette Serine
AU - Mennen, Saskia
AU - Nilsen, Frank
PY - 2009/8
Y1 - 2009/8
N2 - Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96 unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
AB - Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96 unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
KW - Atlantic cod
KW - cDNA
KW - Expressed sequence tags (eSTs)
KW - Gadus morhua
KW - Microsatellites
KW - Type I markers
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U2 - 10.1007/s10592-008-9682-2
DO - 10.1007/s10592-008-9682-2
M3 - Article
VL - 10
SP - 1037
EP - 1040
JO - Conservation Genetics
JF - Conservation Genetics
SN - 1566-0621
IS - 4
ER -