New polymorphic di-nucleotide microsatellite markers for Atlantic cod (Gadus morhua L.)

Madjid Delghandi, Mette Serine Wesmajervi, Saskia Mennen, Frank Nilsen

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2 Citations (Scopus)

Abstract

Twenty three polymorphic microsatellite markers were developed from approximately 2,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Seventy two primer pairs were designed for EST sequences containing perfect di-nucleotide motifs and characterised in 96 unrelated fish. Twenty three markers were successfully amplified with number of alleles from 2 to 18 per locus and observed and expected heterozygosity ranging from 0.03 to 1.00 and 0.04 to 0.90, respectively. Loci Gmo-C280, Gmo-C283, Gmo-C290 and Gmo-C293 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C267 and Gmo-C269 and Gmo-C262 and Gmo-C291. The gene identity was determined at three of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.

Original languageEnglish
Pages (from-to)1037-1040
Number of pages4
JournalConservation Genetics
Volume10
Issue number4
DOIs
Publication statusPublished - Aug 2009

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Keywords

  • Atlantic cod
  • cDNA
  • Expressed sequence tags (eSTs)
  • Gadus morhua
  • Microsatellites
  • Type I markers

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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