Neuroprotective effects of hesperidin, a plant flavanone, on rotenone-induced oxidative stress and apoptosis in a cellular model for Parkinson's disease

Kuppusamy Tamilselvam, Nady Braidy, Thamilarasan Manivasagam, Musthafa Mohamed Essa, Nagarajan Rajendra Prasad, Subburayan Karthikeyan, Arokyasamy Justin Thenmozhi, Subash Selvaraju, Gilles J. Guillemin

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Rotenone a widely used pesticide that inhibits mitochondrial complex I has been used to investigate the pathobiology of PD both in vitro and in vivo. Studies have shown that the neurotoxicity of rotenone may be related to its ability to generate reactive oxygen species (ROS), leading to neuronal apoptosis. The current study was carried out to investigate the neuroprotective effects of hesperidin, a citrus fruit flavanol, against rotenone-induced apoptosis in human neuroblastoma SK-N-SH cells. We assessed cell death, mitochondrial membrane potential, ROS generation, ATP levels, thiobarbituric acid reactive substances, reduced glutathione (GSH) levels, and the activity of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) using well established assays. Apoptosis was determined in normal, rotenone, and hesperidin treated cells, by measuring the protein expression of cytochrome c (cyt c), caspases 3 and 9, Bax, and Bcl-2 using the standard western blotting technique. The apoptosis in rotenone-induced SK-N-SH cells was accompanied by the loss of mitochondrial membrane potential, increased ROS generation, the depletion of GSH, enhanced activities of enzymatic antioxidants, upregulation of Bax, cyt c, and caspases 3 and 9, and downregulation of Bcl-2, which were attenuated in the presence of hesperidin. Our data suggests that hesperidin exerts its neuroprotective effect against rotenone due to its antioxidant, maintenance of mitochondrial function, and antiapoptotic properties in a neuroblastoma cell line.

Original languageEnglish
Article number102741
JournalOxidative Medicine and Cellular Longevity
DOIs
Publication statusPublished - 2013

Fingerprint

Hesperidin
Rotenone
Oxidative stress
Neuroprotective Agents
Parkinson Disease
Oxidative Stress
Apoptosis
Reactive Oxygen Species
Caspase 9
Mitochondrial Membrane Potential
Neuroblastoma
Caspase 3
Citrus fruits
Antioxidants
Cells
Membranes
Thiobarbituric Acid Reactive Substances
Citrus
Cell death
Glutathione Peroxidase

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry
  • Medicine(all)

Cite this

Neuroprotective effects of hesperidin, a plant flavanone, on rotenone-induced oxidative stress and apoptosis in a cellular model for Parkinson's disease. / Tamilselvam, Kuppusamy; Braidy, Nady; Manivasagam, Thamilarasan; Essa, Musthafa Mohamed; Prasad, Nagarajan Rajendra; Karthikeyan, Subburayan; Thenmozhi, Arokyasamy Justin; Selvaraju, Subash; Guillemin, Gilles J.

In: Oxidative Medicine and Cellular Longevity, 2013.

Research output: Contribution to journalArticle

Tamilselvam, Kuppusamy ; Braidy, Nady ; Manivasagam, Thamilarasan ; Essa, Musthafa Mohamed ; Prasad, Nagarajan Rajendra ; Karthikeyan, Subburayan ; Thenmozhi, Arokyasamy Justin ; Selvaraju, Subash ; Guillemin, Gilles J. / Neuroprotective effects of hesperidin, a plant flavanone, on rotenone-induced oxidative stress and apoptosis in a cellular model for Parkinson's disease. In: Oxidative Medicine and Cellular Longevity. 2013.
@article{9c19c8757bd043a0a0701c7fa1ece8af,
title = "Neuroprotective effects of hesperidin, a plant flavanone, on rotenone-induced oxidative stress and apoptosis in a cellular model for Parkinson's disease",
abstract = "Rotenone a widely used pesticide that inhibits mitochondrial complex I has been used to investigate the pathobiology of PD both in vitro and in vivo. Studies have shown that the neurotoxicity of rotenone may be related to its ability to generate reactive oxygen species (ROS), leading to neuronal apoptosis. The current study was carried out to investigate the neuroprotective effects of hesperidin, a citrus fruit flavanol, against rotenone-induced apoptosis in human neuroblastoma SK-N-SH cells. We assessed cell death, mitochondrial membrane potential, ROS generation, ATP levels, thiobarbituric acid reactive substances, reduced glutathione (GSH) levels, and the activity of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) using well established assays. Apoptosis was determined in normal, rotenone, and hesperidin treated cells, by measuring the protein expression of cytochrome c (cyt c), caspases 3 and 9, Bax, and Bcl-2 using the standard western blotting technique. The apoptosis in rotenone-induced SK-N-SH cells was accompanied by the loss of mitochondrial membrane potential, increased ROS generation, the depletion of GSH, enhanced activities of enzymatic antioxidants, upregulation of Bax, cyt c, and caspases 3 and 9, and downregulation of Bcl-2, which were attenuated in the presence of hesperidin. Our data suggests that hesperidin exerts its neuroprotective effect against rotenone due to its antioxidant, maintenance of mitochondrial function, and antiapoptotic properties in a neuroblastoma cell line.",
author = "Kuppusamy Tamilselvam and Nady Braidy and Thamilarasan Manivasagam and Essa, {Musthafa Mohamed} and Prasad, {Nagarajan Rajendra} and Subburayan Karthikeyan and Thenmozhi, {Arokyasamy Justin} and Subash Selvaraju and Guillemin, {Gilles J.}",
year = "2013",
doi = "10.1155/2013/102741",
language = "English",
journal = "Oxidative Medicine and Cellular Longevity",
issn = "1942-0900",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Neuroprotective effects of hesperidin, a plant flavanone, on rotenone-induced oxidative stress and apoptosis in a cellular model for Parkinson's disease

AU - Tamilselvam, Kuppusamy

AU - Braidy, Nady

AU - Manivasagam, Thamilarasan

AU - Essa, Musthafa Mohamed

AU - Prasad, Nagarajan Rajendra

AU - Karthikeyan, Subburayan

AU - Thenmozhi, Arokyasamy Justin

AU - Selvaraju, Subash

AU - Guillemin, Gilles J.

PY - 2013

Y1 - 2013

N2 - Rotenone a widely used pesticide that inhibits mitochondrial complex I has been used to investigate the pathobiology of PD both in vitro and in vivo. Studies have shown that the neurotoxicity of rotenone may be related to its ability to generate reactive oxygen species (ROS), leading to neuronal apoptosis. The current study was carried out to investigate the neuroprotective effects of hesperidin, a citrus fruit flavanol, against rotenone-induced apoptosis in human neuroblastoma SK-N-SH cells. We assessed cell death, mitochondrial membrane potential, ROS generation, ATP levels, thiobarbituric acid reactive substances, reduced glutathione (GSH) levels, and the activity of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) using well established assays. Apoptosis was determined in normal, rotenone, and hesperidin treated cells, by measuring the protein expression of cytochrome c (cyt c), caspases 3 and 9, Bax, and Bcl-2 using the standard western blotting technique. The apoptosis in rotenone-induced SK-N-SH cells was accompanied by the loss of mitochondrial membrane potential, increased ROS generation, the depletion of GSH, enhanced activities of enzymatic antioxidants, upregulation of Bax, cyt c, and caspases 3 and 9, and downregulation of Bcl-2, which were attenuated in the presence of hesperidin. Our data suggests that hesperidin exerts its neuroprotective effect against rotenone due to its antioxidant, maintenance of mitochondrial function, and antiapoptotic properties in a neuroblastoma cell line.

AB - Rotenone a widely used pesticide that inhibits mitochondrial complex I has been used to investigate the pathobiology of PD both in vitro and in vivo. Studies have shown that the neurotoxicity of rotenone may be related to its ability to generate reactive oxygen species (ROS), leading to neuronal apoptosis. The current study was carried out to investigate the neuroprotective effects of hesperidin, a citrus fruit flavanol, against rotenone-induced apoptosis in human neuroblastoma SK-N-SH cells. We assessed cell death, mitochondrial membrane potential, ROS generation, ATP levels, thiobarbituric acid reactive substances, reduced glutathione (GSH) levels, and the activity of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) using well established assays. Apoptosis was determined in normal, rotenone, and hesperidin treated cells, by measuring the protein expression of cytochrome c (cyt c), caspases 3 and 9, Bax, and Bcl-2 using the standard western blotting technique. The apoptosis in rotenone-induced SK-N-SH cells was accompanied by the loss of mitochondrial membrane potential, increased ROS generation, the depletion of GSH, enhanced activities of enzymatic antioxidants, upregulation of Bax, cyt c, and caspases 3 and 9, and downregulation of Bcl-2, which were attenuated in the presence of hesperidin. Our data suggests that hesperidin exerts its neuroprotective effect against rotenone due to its antioxidant, maintenance of mitochondrial function, and antiapoptotic properties in a neuroblastoma cell line.

UR - http://www.scopus.com/inward/record.url?scp=84885641411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885641411&partnerID=8YFLogxK

U2 - 10.1155/2013/102741

DO - 10.1155/2013/102741

M3 - Article

JO - Oxidative Medicine and Cellular Longevity

JF - Oxidative Medicine and Cellular Longevity

SN - 1942-0900

M1 - 102741

ER -