Neuroprotective Effects of a Variety of Pomegranate Juice Extracts (PJE) Against the Excitotoxin Quinolinic Acid in Human Primary Neurons

N Braidy; S Subash; M M Essa; R Vaishnav; S Al-Adawi; A Al-Asmi; H Al-Senawi; A A R Alobaidy; R Lakhtakia; G J Guillemin

doi:10.14283/jpad.2014.3

Neuroprotective Effects of a Variety of Pomegranate Juice Extracts (PJE) Against the Excitotoxin Quinolinic Acid in Human Primary Neurons

N Braidy, S Subash, M M Essa, R Vaishnav, S Al-Adawi, A Al-Asmi, H Al-Senawi, A A R Alobaidy, R Lakhtakia, G J Guillemin

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Quinolinic acid (QUIN) excitotoxicity is mediated by elevated intracellular Ca2+ levels, and nitric oxide (NO•) mediated oxidative stress leading to DNA damage, and cell death due to energy restriction.

METHODS: We evaluated the effect of a series of pomegranate juice extracts (PJE), Helow, Malasi, Qusum, and Hamedh, with antioxidant properties on QUIN induced excitotoxicity on primary cultures of human neurons.

RESULTS: We showed that Helow and Malasi can attenuate QUIN-induced excitotoxicity to a greater extent than Qusum and Hamedh from Oman. Similarly, both Helow and Malasi were able to attenuate QUIN-induced Ca2+ influx and nNOS activity to a greater extent compared to Qusum, and Hamedh. All extracts reduced the oxidative effects of increased NO• production, and hence preventing NAD+ depletion and cell death.

CONCLUSION: In addition to the well-known antioxidant properties of these natural phytochemicals, the inhibitory effect of some of these compounds on specific excitotoxic processes such as calcium influx provides additional evidence for the beneficial health effects of PJE in excitable tissue, particularly within the CNS.

Original language	English
Pages (from-to)	84-90
Number of pages	7
Journal	The journal of prevention of Alzheimer's disease
Volume	1
Issue number	2
DOIs	https://doi.org/10.14283/jpad.2014.3
Publication status	Published - 2014

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Braidy, N., Subash, S., Essa, M. M., Vaishnav, R., Al-Adawi, S., Al-Asmi, A., Al-Senawi, H., Alobaidy, A. A. R., Lakhtakia, R., & Guillemin, G. J. (2014). Neuroprotective Effects of a Variety of Pomegranate Juice Extracts (PJE) Against the Excitotoxin Quinolinic Acid in Human Primary Neurons. The journal of prevention of Alzheimer's disease, 1(2), 84-90. https://doi.org/10.14283/jpad.2014.3

Braidy, N, Subash, S, Essa, MM, Vaishnav, R, Al-Adawi, S, Al-Asmi, A, Al-Senawi, H, Alobaidy, AAR, Lakhtakia, R & Guillemin, GJ 2014, 'Neuroprotective Effects of a Variety of Pomegranate Juice Extracts (PJE) Against the Excitotoxin Quinolinic Acid in Human Primary Neurons', The journal of prevention of Alzheimer's disease, vol. 1, no. 2, pp. 84-90. https://doi.org/10.14283/jpad.2014.3

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title = "Neuroprotective Effects of a Variety of Pomegranate Juice Extracts (PJE) Against the Excitotoxin Quinolinic Acid in Human Primary Neurons",

abstract = "BACKGROUND: Quinolinic acid (QUIN) excitotoxicity is mediated by elevated intracellular Ca2+ levels, and nitric oxide (NO•) mediated oxidative stress leading to DNA damage, and cell death due to energy restriction.METHODS: We evaluated the effect of a series of pomegranate juice extracts (PJE), Helow, Malasi, Qusum, and Hamedh, with antioxidant properties on QUIN induced excitotoxicity on primary cultures of human neurons.RESULTS: We showed that Helow and Malasi can attenuate QUIN-induced excitotoxicity to a greater extent than Qusum and Hamedh from Oman. Similarly, both Helow and Malasi were able to attenuate QUIN-induced Ca2+ influx and nNOS activity to a greater extent compared to Qusum, and Hamedh. All extracts reduced the oxidative effects of increased NO• production, and hence preventing NAD+ depletion and cell death.CONCLUSION: In addition to the well-known antioxidant properties of these natural phytochemicals, the inhibitory effect of some of these compounds on specific excitotoxic processes such as calcium influx provides additional evidence for the beneficial health effects of PJE in excitable tissue, particularly within the CNS.",

author = "N Braidy and S Subash and Essa, {M M} and R Vaishnav and S Al-Adawi and A Al-Asmi and H Al-Senawi and Alobaidy, {A A R} and R Lakhtakia and Guillemin, {G J}",

year = "2014",

doi = "10.14283/jpad.2014.3",

language = "English",

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pages = "84--90",

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AU - Braidy, N

AU - Subash, S

AU - Essa, M M

AU - Vaishnav, R

AU - Al-Adawi, S

AU - Al-Asmi, A

AU - Al-Senawi, H

AU - Alobaidy, A A R

AU - Lakhtakia, R

AU - Guillemin, G J

PY - 2014

Y1 - 2014

N2 - BACKGROUND: Quinolinic acid (QUIN) excitotoxicity is mediated by elevated intracellular Ca2+ levels, and nitric oxide (NO•) mediated oxidative stress leading to DNA damage, and cell death due to energy restriction.METHODS: We evaluated the effect of a series of pomegranate juice extracts (PJE), Helow, Malasi, Qusum, and Hamedh, with antioxidant properties on QUIN induced excitotoxicity on primary cultures of human neurons.RESULTS: We showed that Helow and Malasi can attenuate QUIN-induced excitotoxicity to a greater extent than Qusum and Hamedh from Oman. Similarly, both Helow and Malasi were able to attenuate QUIN-induced Ca2+ influx and nNOS activity to a greater extent compared to Qusum, and Hamedh. All extracts reduced the oxidative effects of increased NO• production, and hence preventing NAD+ depletion and cell death.CONCLUSION: In addition to the well-known antioxidant properties of these natural phytochemicals, the inhibitory effect of some of these compounds on specific excitotoxic processes such as calcium influx provides additional evidence for the beneficial health effects of PJE in excitable tissue, particularly within the CNS.

AB - BACKGROUND: Quinolinic acid (QUIN) excitotoxicity is mediated by elevated intracellular Ca2+ levels, and nitric oxide (NO•) mediated oxidative stress leading to DNA damage, and cell death due to energy restriction.METHODS: We evaluated the effect of a series of pomegranate juice extracts (PJE), Helow, Malasi, Qusum, and Hamedh, with antioxidant properties on QUIN induced excitotoxicity on primary cultures of human neurons.RESULTS: We showed that Helow and Malasi can attenuate QUIN-induced excitotoxicity to a greater extent than Qusum and Hamedh from Oman. Similarly, both Helow and Malasi were able to attenuate QUIN-induced Ca2+ influx and nNOS activity to a greater extent compared to Qusum, and Hamedh. All extracts reduced the oxidative effects of increased NO• production, and hence preventing NAD+ depletion and cell death.CONCLUSION: In addition to the well-known antioxidant properties of these natural phytochemicals, the inhibitory effect of some of these compounds on specific excitotoxic processes such as calcium influx provides additional evidence for the beneficial health effects of PJE in excitable tissue, particularly within the CNS.

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DO - 10.14283/jpad.2014.3

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C2 - 29255836

SN - 2426-0266

VL - 1

SP - 84

EP - 90

JO - The journal of prevention of Alzheimer's disease

JF - The journal of prevention of Alzheimer's disease

IS - 2

ER -

Neuroprotective Effects of a Variety of Pomegranate Juice Extracts (PJE) Against the Excitotoxin Quinolinic Acid in Human Primary Neurons

Abstract

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