Neuroprotective effect of asiatic acid on rotenone-induced mitochondrial dysfunction and oxidative stress-mediated apoptosis in differentiated SH-SYS5Y cells

Jagatheesan Nataraj, Thamilarasan Manivasagam, Arokiasamy Justin Thenmozhi, Musthafa Mohamed Essa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Parkinson's disease (PD) is a chronic neurodegenerative disease, manifested due to the loss of dopaminergic neurons, which ultimately leads to impaired movement in elderly populations. The pathogenesis of PD is associated with numerous factors including oxidative stress, mitochondrial dysfunction and apoptosis. There is no effective therapy available to cure or halt the progression of this disease still now. Asiatic acid (AA) is a triterpene extracted from Centella asiatica has been reported as an antioxidant and anti-inflammatory agent, that offers neuroprotection against glutamate toxicity. Therefore, in this study, we have investigated the effect of AA in a rotenone (an inhibitor of mitochondrial complex I) induced in vitro model of PD. Following the exposure of SH-SY5Y cells to rotenone, there was a marked overproduction of ROS, mitochondrial dysfunction (as indexed by the decrease in mitochondrial membrane potential) and apoptosis (Hoechst and dual staining, comet assay; expressions of pro-apoptotic and anti-apoptotic indices). Pre-treatment with AA reversed these changes might be due to its antioxidant, mitoprotective and anti-apoptotic properties. However further extensive studies on in vivo models of PD are warranted to prove AA neuroprotective effect before entering into the clinical trial.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalNutritional Neuroscience
DOIs
Publication statusAccepted/In press - Feb 9 2016

Fingerprint

Rotenone
Neuroprotective Agents
Parkinson Disease
Oxidative Stress
Apoptosis
Antioxidants
Centella
Triterpenes
Comet Assay
Mitochondrial Membrane Potential
Dopaminergic Neurons
Neurodegenerative Diseases
Disease Progression
Glutamic Acid
Chronic Disease
Anti-Inflammatory Agents
Clinical Trials
Staining and Labeling
asiatic acid
Therapeutics

Keywords

  • Apoptosis
  • Mitochondrial dysfunction
  • Oxidative stress
  • Parkinson's disease
  • Rotenone

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Neuroscience(all)

Cite this

Neuroprotective effect of asiatic acid on rotenone-induced mitochondrial dysfunction and oxidative stress-mediated apoptosis in differentiated SH-SYS5Y cells. / Nataraj, Jagatheesan; Manivasagam, Thamilarasan; Justin Thenmozhi, Arokiasamy; Essa, Musthafa Mohamed.

In: Nutritional Neuroscience, 09.02.2016, p. 1-9.

Research output: Contribution to journalArticle

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