NESTROFT as a screening test for the detection of thalassaemia and common haemoglobinopathies - An evaluation against a high performance liquid chromatographic method

Susanna Thomas, Alok Srivastava, L. Jeyaseelan, David Dennison, Mammen Chandy

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The NESTROFT method (Naked Eye Single Tube Red Cell Osmotic Fragility Test) was evaluated against a high performance liquid chromatographic (HPLC) method for its usefulness in screening for β-thalassaemia and some of the common haemoglobinopathies. Blood samples (137) from patients with suspected haemoglobin disorders were analyzed by both methods. Among the 63 patients with heterozygous β-thalassaemia on HPLC, NESTROFT was positive for 49,'doubtful' for 13 and negative for 1. Of the 32 patients with other haemoglobin disorders, 28 were positive on NESTROFT and 4 were 'doubtful'. Of the 42 'normal' samples, NESTROFT was positive for 6, 'doubtful' for 8 and negative for 28. This test showed an overall sensitivity of 98.7 per cent, specificity of 66.6 per cent, positive predictive value of 87 per cent and negative predictive value of 96.5 per cent. We conclude that, NESTROFT is a suitable test for screening for β-thalassaemia and the common haemoglobinopathies seen in India. It is easy to perform, simple, inexpensive and does not require sophisticated equipment. Subjects who are NESTROFT 'positive' or 'doubtful' deserve further investigation.

Original languageEnglish
Pages (from-to)194-197
Number of pages4
JournalIndian Journal of Medical Research
Volume104
Issue numberAUG.
Publication statusPublished - Aug 1996

Fingerprint

Hemoglobinopathies
Thalassemia
Screening
Hemoglobins
Liquids
Osmotic Fragility
Blood
Cells
India
Equipment and Supplies

Keywords

  • Haemoglobinopathy
  • High performance liquid chromatography
  • NESTROFT
  • Thalassaemia

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

NESTROFT as a screening test for the detection of thalassaemia and common haemoglobinopathies - An evaluation against a high performance liquid chromatographic method. / Thomas, Susanna; Srivastava, Alok; Jeyaseelan, L.; Dennison, David; Chandy, Mammen.

In: Indian Journal of Medical Research, Vol. 104, No. AUG., 08.1996, p. 194-197.

Research output: Contribution to journalArticle

@article{b0910fdbd70e4183b922f8509c1e7dde,
title = "NESTROFT as a screening test for the detection of thalassaemia and common haemoglobinopathies - An evaluation against a high performance liquid chromatographic method",
abstract = "The NESTROFT method (Naked Eye Single Tube Red Cell Osmotic Fragility Test) was evaluated against a high performance liquid chromatographic (HPLC) method for its usefulness in screening for β-thalassaemia and some of the common haemoglobinopathies. Blood samples (137) from patients with suspected haemoglobin disorders were analyzed by both methods. Among the 63 patients with heterozygous β-thalassaemia on HPLC, NESTROFT was positive for 49,'doubtful' for 13 and negative for 1. Of the 32 patients with other haemoglobin disorders, 28 were positive on NESTROFT and 4 were 'doubtful'. Of the 42 'normal' samples, NESTROFT was positive for 6, 'doubtful' for 8 and negative for 28. This test showed an overall sensitivity of 98.7 per cent, specificity of 66.6 per cent, positive predictive value of 87 per cent and negative predictive value of 96.5 per cent. We conclude that, NESTROFT is a suitable test for screening for β-thalassaemia and the common haemoglobinopathies seen in India. It is easy to perform, simple, inexpensive and does not require sophisticated equipment. Subjects who are NESTROFT 'positive' or 'doubtful' deserve further investigation.",
keywords = "Haemoglobinopathy, High performance liquid chromatography, NESTROFT, Thalassaemia",
author = "Susanna Thomas and Alok Srivastava and L. Jeyaseelan and David Dennison and Mammen Chandy",
year = "1996",
month = "8",
language = "English",
volume = "104",
pages = "194--197",
journal = "Indian Journal of Medical Research",
issn = "0971-5916",
publisher = "Indian Council of Medical Research",
number = "AUG.",

}

TY - JOUR

T1 - NESTROFT as a screening test for the detection of thalassaemia and common haemoglobinopathies - An evaluation against a high performance liquid chromatographic method

AU - Thomas, Susanna

AU - Srivastava, Alok

AU - Jeyaseelan, L.

AU - Dennison, David

AU - Chandy, Mammen

PY - 1996/8

Y1 - 1996/8

N2 - The NESTROFT method (Naked Eye Single Tube Red Cell Osmotic Fragility Test) was evaluated against a high performance liquid chromatographic (HPLC) method for its usefulness in screening for β-thalassaemia and some of the common haemoglobinopathies. Blood samples (137) from patients with suspected haemoglobin disorders were analyzed by both methods. Among the 63 patients with heterozygous β-thalassaemia on HPLC, NESTROFT was positive for 49,'doubtful' for 13 and negative for 1. Of the 32 patients with other haemoglobin disorders, 28 were positive on NESTROFT and 4 were 'doubtful'. Of the 42 'normal' samples, NESTROFT was positive for 6, 'doubtful' for 8 and negative for 28. This test showed an overall sensitivity of 98.7 per cent, specificity of 66.6 per cent, positive predictive value of 87 per cent and negative predictive value of 96.5 per cent. We conclude that, NESTROFT is a suitable test for screening for β-thalassaemia and the common haemoglobinopathies seen in India. It is easy to perform, simple, inexpensive and does not require sophisticated equipment. Subjects who are NESTROFT 'positive' or 'doubtful' deserve further investigation.

AB - The NESTROFT method (Naked Eye Single Tube Red Cell Osmotic Fragility Test) was evaluated against a high performance liquid chromatographic (HPLC) method for its usefulness in screening for β-thalassaemia and some of the common haemoglobinopathies. Blood samples (137) from patients with suspected haemoglobin disorders were analyzed by both methods. Among the 63 patients with heterozygous β-thalassaemia on HPLC, NESTROFT was positive for 49,'doubtful' for 13 and negative for 1. Of the 32 patients with other haemoglobin disorders, 28 were positive on NESTROFT and 4 were 'doubtful'. Of the 42 'normal' samples, NESTROFT was positive for 6, 'doubtful' for 8 and negative for 28. This test showed an overall sensitivity of 98.7 per cent, specificity of 66.6 per cent, positive predictive value of 87 per cent and negative predictive value of 96.5 per cent. We conclude that, NESTROFT is a suitable test for screening for β-thalassaemia and the common haemoglobinopathies seen in India. It is easy to perform, simple, inexpensive and does not require sophisticated equipment. Subjects who are NESTROFT 'positive' or 'doubtful' deserve further investigation.

KW - Haemoglobinopathy

KW - High performance liquid chromatography

KW - NESTROFT

KW - Thalassaemia

UR - http://www.scopus.com/inward/record.url?scp=0029790679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029790679&partnerID=8YFLogxK

M3 - Article

VL - 104

SP - 194

EP - 197

JO - Indian Journal of Medical Research

JF - Indian Journal of Medical Research

SN - 0971-5916

IS - AUG.

ER -