Multiple nuclear and mitochondrial genotyping identifies emperors and large-eye breams (Teleostei: Lethrinidae) from New Caledonia and reveals new large-eye bream species

Philippe Borsa, Adeline Collet, Laure Carassou, Dominique Ponton, Wei Jen Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Species identification is fundamental to address questions about community ecology, biodiversity, conservation and resource management, at any life history stage. Current studies on fish larval ecology of tropical species are hampered by the lack of reliable and effective tools for identifying larvae at the species level. Emperors and large-eye breams comprise fish species from the perciform fish family Lethrinidae. They inhabit coastal and coral-reef habitats of the tropical Indo-Pacific, and they are important fishery resources. Their taxonomy is considered difficult and identification to species is often problematic. Lethrinidae larvae and juveniles can be identified on the basis of meristic counts at the sub-family level, but no further. In this study, we developed a set of polymorphic PCR markers (size polymorphisms at the intron regions from 4/5 nuclear protein-coding genes and single-strand conformation polymorphism of a 205-bp fragment at the mitochondrial 16S rRNA locus), to characterize 341 specimens from 21 Lethrinidae species from New Caledonia (southwestern tropical Pacific). A genetic data-bank was constructed using the genotypes screened from the multiple gene loci of adult or sub-adult specimens used as references for these species. The 16S rRNA gene fragment was able to differentiate species for the genus Lethrinus, but it provided little diagnostic resolution among different species within the genus Gymnocranius. A combination of the 16S rRNA marker and 4 nuclear markers developed herein allowed to sort out species within Gymnocranius spp. from New Caledonia. Using genotype distributions at nuclear loci to test for reproductive isolation, we found that three apparently undescribed large-eye bream species may exist, provisionally referred to as Gymnocranius sp. A, sp. B and sp. C. Subsequent genotyping of 137 Lethrinidae larvae collected from the bays of the Noumea peninsula, New Caledonia, found a total of three species (Lethrinus genivittatus, Lethrinus olivaceus and Gymnocranius sp. A).

Original languageEnglish
Pages (from-to)370-389
Number of pages20
JournalBiochemical Systematics and Ecology
Volume38
Issue number3
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Lethrinidae
New Caledonia
Lethrinus
bream
Fish
genotyping
Larva
Fishes
Genes
eyes
Ecology
Polymorphism
ribosomal RNA
Genotype
Coral Reefs
Reproductive Isolation
loci
Genetic Databases
larvae
Fisheries

Keywords

  • Biological species concept
  • Cryptic species
  • Fish
  • Identification
  • Indo-Pacific
  • Larvae
  • PCR markers

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Multiple nuclear and mitochondrial genotyping identifies emperors and large-eye breams (Teleostei : Lethrinidae) from New Caledonia and reveals new large-eye bream species. / Borsa, Philippe; Collet, Adeline; Carassou, Laure; Ponton, Dominique; Chen, Wei Jen.

In: Biochemical Systematics and Ecology, Vol. 38, No. 3, 06.2010, p. 370-389.

Research output: Contribution to journalArticle

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abstract = "Species identification is fundamental to address questions about community ecology, biodiversity, conservation and resource management, at any life history stage. Current studies on fish larval ecology of tropical species are hampered by the lack of reliable and effective tools for identifying larvae at the species level. Emperors and large-eye breams comprise fish species from the perciform fish family Lethrinidae. They inhabit coastal and coral-reef habitats of the tropical Indo-Pacific, and they are important fishery resources. Their taxonomy is considered difficult and identification to species is often problematic. Lethrinidae larvae and juveniles can be identified on the basis of meristic counts at the sub-family level, but no further. In this study, we developed a set of polymorphic PCR markers (size polymorphisms at the intron regions from 4/5 nuclear protein-coding genes and single-strand conformation polymorphism of a 205-bp fragment at the mitochondrial 16S rRNA locus), to characterize 341 specimens from 21 Lethrinidae species from New Caledonia (southwestern tropical Pacific). A genetic data-bank was constructed using the genotypes screened from the multiple gene loci of adult or sub-adult specimens used as references for these species. The 16S rRNA gene fragment was able to differentiate species for the genus Lethrinus, but it provided little diagnostic resolution among different species within the genus Gymnocranius. A combination of the 16S rRNA marker and 4 nuclear markers developed herein allowed to sort out species within Gymnocranius spp. from New Caledonia. Using genotype distributions at nuclear loci to test for reproductive isolation, we found that three apparently undescribed large-eye bream species may exist, provisionally referred to as Gymnocranius sp. A, sp. B and sp. C. Subsequent genotyping of 137 Lethrinidae larvae collected from the bays of the Noumea peninsula, New Caledonia, found a total of three species (Lethrinus genivittatus, Lethrinus olivaceus and Gymnocranius sp. A).",
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AU - Ponton, Dominique

AU - Chen, Wei Jen

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