TY - JOUR
T1 - Multigene characterization of a ‘Candidatus Phytoplasma australasia’ strain associated with Roystonea regia in Oman
AU - Hemmati, Chamran
AU - Al-Subhi, Ali Masood
AU - Al-Sadi, Abdullah Mohammed
N1 - Publisher Copyright:
© 2020 The Canadian Phytopathological Society.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Florida or Cuban royal palm (Roystonea regia (Kunth) O.F. Cook) is an important ornamental plant with many uses for humans. Royal palm trees grown in Muscat, Oman, were found exhibiting leaf yellowing, leaf decay starting on the mature leaves, and leaf stunting. Trunk tissue samples were collected and analysed with nested and semi-nested PCR assays for the 16S rRNA, translation elongation factor Tu (tuf), and immunodominant membrane protein (imp) genes, in order to characterize the phytoplasma associated with the affected trees. Sequence and phylogenetic analysis of the 16S rRNA, tuf, and imp genes indicated that the phytoplasma strains detected in the trees were identical and belonged to the 16SrII-D subgroup. This is the first report of an association between ‘Candidatus Phytoplasma asutralasia’-related strain and R. regia.
AB - Florida or Cuban royal palm (Roystonea regia (Kunth) O.F. Cook) is an important ornamental plant with many uses for humans. Royal palm trees grown in Muscat, Oman, were found exhibiting leaf yellowing, leaf decay starting on the mature leaves, and leaf stunting. Trunk tissue samples were collected and analysed with nested and semi-nested PCR assays for the 16S rRNA, translation elongation factor Tu (tuf), and immunodominant membrane protein (imp) genes, in order to characterize the phytoplasma associated with the affected trees. Sequence and phylogenetic analysis of the 16S rRNA, tuf, and imp genes indicated that the phytoplasma strains detected in the trees were identical and belonged to the 16SrII-D subgroup. This is the first report of an association between ‘Candidatus Phytoplasma asutralasia’-related strain and R. regia.
KW - 16SrII-D
KW - identification
KW - molecular
KW - phytoplasma detection
KW - royal palm
UR - https://www.mendeley.com/catalogue/228a5e58-9638-35fe-953f-086f5ba52e1f/
U2 - 10.1080/07060661.2020.1833248
DO - 10.1080/07060661.2020.1833248
M3 - Article
AN - SCOPUS:85096136479
SN - 0706-0661
VL - 43
SP - 374
EP - 383
JO - Canadian Journal of Plant Pathology
JF - Canadian Journal of Plant Pathology
ER -