Multigene characterization of a ‘Candidatus Phytoplasma australasia’ strain associated with Roystonea regia in Oman

Chamran Hemmati; Ali Masood Al-Subhi; Abdullah Mohammed Al-Sadi

doi:10.1080/07060661.2020.1833248

Multigene characterization of a ‘Candidatus Phytoplasma australasia’ strain associated with Roystonea regia in Oman

Chamran Hemmati, Ali Masood Al-Subhi, Abdullah Mohammed Al-Sadi^*

^*Corresponding author for this work

Crop Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Florida or Cuban royal palm (Roystonea regia (Kunth) O.F. Cook) is an important ornamental plant with many uses for humans. Royal palm trees grown in Muscat, Oman, were found exhibiting leaf yellowing, leaf decay starting on the mature leaves, and leaf stunting. Trunk tissue samples were collected and analysed with nested and semi-nested PCR assays for the 16S rRNA, translation elongation factor Tu (tuf), and immunodominant membrane protein (imp) genes, in order to characterize the phytoplasma associated with the affected trees. Sequence and phylogenetic analysis of the 16S rRNA, tuf, and imp genes indicated that the phytoplasma strains detected in the trees were identical and belonged to the 16SrII-D subgroup. This is the first report of an association between ‘Candidatus Phytoplasma asutralasia’-related strain and R. regia.

Original language	English
Pages (from-to)	374-383
Number of pages	10
Journal	Canadian Journal of Plant Pathology
Volume	43
Issue number	3
DOIs	https://doi.org/10.1080/07060661.2020.1833248
Publication status	Published - 2020

Keywords

16SrII-D
identification
molecular
phytoplasma detection
royal palm

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1080/07060661.2020.1833248

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@article{f11e8cbe7f3e4ad0820a4fe1b717c265,

title = "Multigene characterization of a {\textquoteleft}Candidatus Phytoplasma australasia{\textquoteright} strain associated with Roystonea regia in Oman",

abstract = "Florida or Cuban royal palm (Roystonea regia (Kunth) O.F. Cook) is an important ornamental plant with many uses for humans. Royal palm trees grown in Muscat, Oman, were found exhibiting leaf yellowing, leaf decay starting on the mature leaves, and leaf stunting. Trunk tissue samples were collected and analysed with nested and semi-nested PCR assays for the 16S rRNA, translation elongation factor Tu (tuf), and immunodominant membrane protein (imp) genes, in order to characterize the phytoplasma associated with the affected trees. Sequence and phylogenetic analysis of the 16S rRNA, tuf, and imp genes indicated that the phytoplasma strains detected in the trees were identical and belonged to the 16SrII-D subgroup. This is the first report of an association between {\textquoteleft}Candidatus Phytoplasma asutralasia{\textquoteright}-related strain and R. regia.",

keywords = "16SrII-D, identification, molecular, phytoplasma detection, royal palm",

author = "Chamran Hemmati and Al-Subhi, {Ali Masood} and Al-Sadi, {Abdullah Mohammed}",

year = "2020",

doi = "10.1080/07060661.2020.1833248",

language = "English",

volume = "43",

pages = "374--383",

journal = "Canadian Journal of Plant Pathology",

issn = "0706-0661",

publisher = "Taylor and Francis Ltd.",

number = "3",

}

TY - JOUR

T1 - Multigene characterization of a ‘Candidatus Phytoplasma australasia’ strain associated with Roystonea regia in Oman

AU - Hemmati, Chamran

AU - Al-Subhi, Ali Masood

AU - Al-Sadi, Abdullah Mohammed

PY - 2020

Y1 - 2020

N2 - Florida or Cuban royal palm (Roystonea regia (Kunth) O.F. Cook) is an important ornamental plant with many uses for humans. Royal palm trees grown in Muscat, Oman, were found exhibiting leaf yellowing, leaf decay starting on the mature leaves, and leaf stunting. Trunk tissue samples were collected and analysed with nested and semi-nested PCR assays for the 16S rRNA, translation elongation factor Tu (tuf), and immunodominant membrane protein (imp) genes, in order to characterize the phytoplasma associated with the affected trees. Sequence and phylogenetic analysis of the 16S rRNA, tuf, and imp genes indicated that the phytoplasma strains detected in the trees were identical and belonged to the 16SrII-D subgroup. This is the first report of an association between ‘Candidatus Phytoplasma asutralasia’-related strain and R. regia.

AB - Florida or Cuban royal palm (Roystonea regia (Kunth) O.F. Cook) is an important ornamental plant with many uses for humans. Royal palm trees grown in Muscat, Oman, were found exhibiting leaf yellowing, leaf decay starting on the mature leaves, and leaf stunting. Trunk tissue samples were collected and analysed with nested and semi-nested PCR assays for the 16S rRNA, translation elongation factor Tu (tuf), and immunodominant membrane protein (imp) genes, in order to characterize the phytoplasma associated with the affected trees. Sequence and phylogenetic analysis of the 16S rRNA, tuf, and imp genes indicated that the phytoplasma strains detected in the trees were identical and belonged to the 16SrII-D subgroup. This is the first report of an association between ‘Candidatus Phytoplasma asutralasia’-related strain and R. regia.

KW - 16SrII-D

KW - identification

KW - molecular

KW - phytoplasma detection

KW - royal palm

UR - https://www.mendeley.com/catalogue/228a5e58-9638-35fe-953f-086f5ba52e1f/

U2 - 10.1080/07060661.2020.1833248

DO - 10.1080/07060661.2020.1833248

M3 - Article

AN - SCOPUS:85096136479

SN - 0706-0661

VL - 43

SP - 374

EP - 383

JO - Canadian Journal of Plant Pathology

JF - Canadian Journal of Plant Pathology

IS - 3

ER -

Multigene characterization of a ‘Candidatus Phytoplasma australasia’ strain associated with Roystonea regia in Oman

Abstract

Keywords

ASJC Scopus subject areas

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