Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco)

Sadaf Altaf, Muhammad Mumtaz Khan, Muhammad Jaffar Jaskani, Iqrar Ahmad Khan, Muhammad Usman, Bushra Sadia, Faisal Saeed Awan, Asif Ali, Azeem Iqbal Khan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Kinnow (Citrus reticulata Blanco) mandarin is a superior fruit crop in Pakistan; however, it is characterized by high number of seeds which is disliked by consumers and the processing industry. The objective of this work was to document the morphological and genetic differences in selected seeded and seedless Kinnow strains from two different locations i.e., Citrus Research Institute Sargodha and University of Agriculture, Faisalabad. The morphological characters suggested variations in external and internal characters of seeded and seedless fruits such as growth habit, fruit shape and texture. Likewise chemical composition of seedless fruit was different than seeded including TSS and Ascorbic acid. Whereas PCA analysis suggested fruit weight, peel weight, non-reducing sugars and fruit diameter were largely contributing for the variations among the seeded and seedless strains of Faisalabad and Sargodha regions. The genetic characterization was carried out using Random Amplification of Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) markers. Two unique fragments of sizes 1000 bp each were detected by RAPD primers GL A-2 and GL C-19 in seedless strains which can be used as a fingerprint for the identification of seedless plants. The results obtained from SSR analysis showed 81.62% of overall polymorphism in seeded and seedless strains of two locations. This variation will help to develop molecular markers for seedless Kinnow strains.

Original languageEnglish
Pages (from-to)1542-1549
Number of pages8
JournalAustralian Journal of Crop Science
Volume8
Issue number11
Publication statusPublished - 2014

Fingerprint

seedless varieties
Citrus reticulata
mandarins
fruits
microsatellite repeats
nonreducing sugars
fruit crops
DNA primers
growth habit
agricultural colleges
Pakistan
Citrus
ascorbic acid
texture
chemical composition
genetic polymorphism
industry
genetic markers
DNA
seeds

Keywords

  • Citrus
  • Genetic characterization
  • Microsatellites
  • Molecular markers
  • Seedlessness

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Altaf, S., Khan, M. M., Jaskani, M. J., Khan, I. A., Usman, M., Sadia, B., ... Khan, A. I. (2014). Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco). Australian Journal of Crop Science, 8(11), 1542-1549.

Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco). / Altaf, Sadaf; Khan, Muhammad Mumtaz; Jaskani, Muhammad Jaffar; Khan, Iqrar Ahmad; Usman, Muhammad; Sadia, Bushra; Awan, Faisal Saeed; Ali, Asif; Khan, Azeem Iqbal.

In: Australian Journal of Crop Science, Vol. 8, No. 11, 2014, p. 1542-1549.

Research output: Contribution to journalArticle

Altaf, S, Khan, MM, Jaskani, MJ, Khan, IA, Usman, M, Sadia, B, Awan, FS, Ali, A & Khan, AI 2014, 'Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco)', Australian Journal of Crop Science, vol. 8, no. 11, pp. 1542-1549.
Altaf, Sadaf ; Khan, Muhammad Mumtaz ; Jaskani, Muhammad Jaffar ; Khan, Iqrar Ahmad ; Usman, Muhammad ; Sadia, Bushra ; Awan, Faisal Saeed ; Ali, Asif ; Khan, Azeem Iqbal. / Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco). In: Australian Journal of Crop Science. 2014 ; Vol. 8, No. 11. pp. 1542-1549.
@article{feeaee93d7fe4b97a8b66ed7f23bc478,
title = "Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco)",
abstract = "Kinnow (Citrus reticulata Blanco) mandarin is a superior fruit crop in Pakistan; however, it is characterized by high number of seeds which is disliked by consumers and the processing industry. The objective of this work was to document the morphological and genetic differences in selected seeded and seedless Kinnow strains from two different locations i.e., Citrus Research Institute Sargodha and University of Agriculture, Faisalabad. The morphological characters suggested variations in external and internal characters of seeded and seedless fruits such as growth habit, fruit shape and texture. Likewise chemical composition of seedless fruit was different than seeded including TSS and Ascorbic acid. Whereas PCA analysis suggested fruit weight, peel weight, non-reducing sugars and fruit diameter were largely contributing for the variations among the seeded and seedless strains of Faisalabad and Sargodha regions. The genetic characterization was carried out using Random Amplification of Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) markers. Two unique fragments of sizes 1000 bp each were detected by RAPD primers GL A-2 and GL C-19 in seedless strains which can be used as a fingerprint for the identification of seedless plants. The results obtained from SSR analysis showed 81.62{\%} of overall polymorphism in seeded and seedless strains of two locations. This variation will help to develop molecular markers for seedless Kinnow strains.",
keywords = "Citrus, Genetic characterization, Microsatellites, Molecular markers, Seedlessness",
author = "Sadaf Altaf and Khan, {Muhammad Mumtaz} and Jaskani, {Muhammad Jaffar} and Khan, {Iqrar Ahmad} and Muhammad Usman and Bushra Sadia and Awan, {Faisal Saeed} and Asif Ali and Khan, {Azeem Iqbal}",
year = "2014",
language = "English",
volume = "8",
pages = "1542--1549",
journal = "Australian Journal of Crop Science",
issn = "1835-2693",
publisher = "Southern Cross Publishing and Printing Pty Ltd",
number = "11",

}

TY - JOUR

T1 - Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco)

AU - Altaf, Sadaf

AU - Khan, Muhammad Mumtaz

AU - Jaskani, Muhammad Jaffar

AU - Khan, Iqrar Ahmad

AU - Usman, Muhammad

AU - Sadia, Bushra

AU - Awan, Faisal Saeed

AU - Ali, Asif

AU - Khan, Azeem Iqbal

PY - 2014

Y1 - 2014

N2 - Kinnow (Citrus reticulata Blanco) mandarin is a superior fruit crop in Pakistan; however, it is characterized by high number of seeds which is disliked by consumers and the processing industry. The objective of this work was to document the morphological and genetic differences in selected seeded and seedless Kinnow strains from two different locations i.e., Citrus Research Institute Sargodha and University of Agriculture, Faisalabad. The morphological characters suggested variations in external and internal characters of seeded and seedless fruits such as growth habit, fruit shape and texture. Likewise chemical composition of seedless fruit was different than seeded including TSS and Ascorbic acid. Whereas PCA analysis suggested fruit weight, peel weight, non-reducing sugars and fruit diameter were largely contributing for the variations among the seeded and seedless strains of Faisalabad and Sargodha regions. The genetic characterization was carried out using Random Amplification of Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) markers. Two unique fragments of sizes 1000 bp each were detected by RAPD primers GL A-2 and GL C-19 in seedless strains which can be used as a fingerprint for the identification of seedless plants. The results obtained from SSR analysis showed 81.62% of overall polymorphism in seeded and seedless strains of two locations. This variation will help to develop molecular markers for seedless Kinnow strains.

AB - Kinnow (Citrus reticulata Blanco) mandarin is a superior fruit crop in Pakistan; however, it is characterized by high number of seeds which is disliked by consumers and the processing industry. The objective of this work was to document the morphological and genetic differences in selected seeded and seedless Kinnow strains from two different locations i.e., Citrus Research Institute Sargodha and University of Agriculture, Faisalabad. The morphological characters suggested variations in external and internal characters of seeded and seedless fruits such as growth habit, fruit shape and texture. Likewise chemical composition of seedless fruit was different than seeded including TSS and Ascorbic acid. Whereas PCA analysis suggested fruit weight, peel weight, non-reducing sugars and fruit diameter were largely contributing for the variations among the seeded and seedless strains of Faisalabad and Sargodha regions. The genetic characterization was carried out using Random Amplification of Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) markers. Two unique fragments of sizes 1000 bp each were detected by RAPD primers GL A-2 and GL C-19 in seedless strains which can be used as a fingerprint for the identification of seedless plants. The results obtained from SSR analysis showed 81.62% of overall polymorphism in seeded and seedless strains of two locations. This variation will help to develop molecular markers for seedless Kinnow strains.

KW - Citrus

KW - Genetic characterization

KW - Microsatellites

KW - Molecular markers

KW - Seedlessness

UR - http://www.scopus.com/inward/record.url?scp=84918496712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84918496712&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 1542

EP - 1549

JO - Australian Journal of Crop Science

JF - Australian Journal of Crop Science

SN - 1835-2693

IS - 11

ER -