Molecular identification of isolates of Peronosclerospora sorghi from maize using PCR-based SCAR marker

D. Ladhalakshmi, A. Vijayasamundeeswari, V. Paranidharan, R. Samiyappan, R. Velazhahan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The fungus Peronosclerospora sorghi [Weston and Uppal (Shaw)] infects both sorghum and maize and incites downy mildew disease. Pathogenic and molecular variability among isolates of P. sorghi from sorghum and maize has been reported. In the present study we developed a DNA sequence characterized amplified region (SCAR) marker for identification of isolates of P. sorghi from maize by using polymerase chain reaction (PCR). The random amplified polymorphic DNA (RAPD) primer OPB15 consistently amplified a 1,000 base pairs (bp) product in PCR only from DNA of P. sorghi isolates from maize and not from isolates of sorghum. The PCR-amplified 1,000-bp product was cloned and sequenced. The sequence of the SCAR marker was used for designing specific primers for identification of maize isolates of P. sorghi. The SCAR primers amplified a 800 bp fragment only from genomic DNA of maize isolates of P. sorghi. The SCAR primers developed in this study are highly specific and reproducible, and proved to be powerful tool for identification of P. sorghi isolates from maize.

Original languageEnglish
Pages (from-to)2129-2135
Number of pages7
JournalWorld Journal of Microbiology and Biotechnology
Volume25
Issue number12
DOIs
Publication statusPublished - Nov 2009

Keywords

  • Downy mildew
  • Maize
  • Molecular detection
  • Peronosclerospora sorghi
  • SCAR marker
  • Sorghum

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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