Molecular genetic analysis of the BRCA2 tumor suppressor gene region in cutaneous squamous cell carcinomas

Sarah E. Gray, Elaine Kay, Mary Leader, Mohamed Mabruk

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Germ line mutations of the BRCA2 tumor suppressor gene with subsequent loss of the remaining wild-type BRCA2 allele have been identified in up to 35% of familial breast cancer cases. A high frequency of allelic loss at the BRCA2 gene locus has also been reported in a variety of sporadic epithelial tumors including oesophageal squamous cell carcinomas (SCC), and sporadic head and neck SCC. Aim: The present study aimed to examine the integrity of the BRCA2 gene in cutaneous SCC. Materials and methods: Allelic imbalance/loss of heterozygosity (AI/LOH) was examined in 22 histologically confirmed cutaneous SCC at two microsatellite markers, D13S260 (centromeric to the BRCA2 gene) and D13S267 (telomeric to the BRCA2 gene). Immunohistochemical analysis of BRCA2 protein expression was also examined in the cutaneous SCC. Results: AI/LOH at the D13S260 locus was found in eight of the 19 informative SCC, and AI/LOH at the D13S267 locus was found in 12 of the 18 informative SCC. Seven SCC showed allelic loss at both markers, and six SCC showed retention of heterozygosity at both markers. Expression of BRCA2 protein was only detected in six of the normal epidermises and three of the 21 SCC examined. Conclusion: AI/LOH of the BRCA2 gene region was found to be common in the cutaneous SCC.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalJournal of Cutaneous Pathology
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2008

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Loss of Heterozygosity
Tumor Suppressor Genes
Molecular Biology
Squamous Cell Carcinoma
BRCA2 Gene
Skin
Allelic Imbalance
BRCA2 Protein
Germ-Line Mutation
Epidermis
Microsatellite Repeats
Alleles

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine

Cite this

Molecular genetic analysis of the BRCA2 tumor suppressor gene region in cutaneous squamous cell carcinomas. / Gray, Sarah E.; Kay, Elaine; Leader, Mary; Mabruk, Mohamed.

In: Journal of Cutaneous Pathology, Vol. 35, No. 1, 01.2008, p. 1-9.

Research output: Contribution to journalArticle

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