Molecular evidence of helicobacter pylori infection in prostate tumors

Mohammed S. Al-Marhoon, Allal Ouhtit, Aisha O. Al-Abri, Krishna P. Venkiteswaran, Qassim Al-Busaidi, Josephkunju Mathew, Ibrahim Al-Haddabi, Omar Shareef, Shahid Aquil, Khalid Rahman, Intisar Al-Hashmi, Ishita Gupta, Shyam S. Ganguly

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: To determine whether Helicobacter pylori (H. pylori) is detectable in both benign prostatic hyperplasia (BPH) and prostate cancer (PCa). Epidemiological studies have shown significant associations between infective chronic prostatitis and prostatic carcinoma. Many bacteria have been found in the prostate of patients with chronic prostatitis, BPH, and PCa. Methods: One hundred consecutive patients with prostate diseases were enrolled in the study. Detection of H. pylori DNA in prostate tissue from patients with BPH and PCa was performed using both immunohistochemistry and PCR, and the results were confirmed by DNA sequencing. Odds ratios and the Fisher Exact test were used for the analysis of the associations between the variables. Results: Among the patients, 78% had BPH and 19% had PCa. While immunohistochemistry showed no positive sample for H. pylori, PCR combined with sequencing detected H. pylori DNA in prostate tissue samples from 5 patients. However, statistical analysis of the data showed that BPH and PCa are not significantly associated with the presence of H. pylori DNA in prostate tissue (odds ratio = 0.94, 95% confidence interval = 0.09-23.34, one-tailed Chi-square value = 0.660, p > 0.05). The limitation of this study was the small number of PCa patients. Conclusions: This study provides, for the first time, molecular evidence of the presence of H. pylori DNA in prostatic tissue of patients with BPH and PCa. It paves the way for further comprehensive studies to examine the association of H. pylori infection with BPH and PCa.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalCurrent Urology
Volume8
DOIs
Publication statusPublished - Sep 4 2015

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Helicobacter Infections
Helicobacter pylori
Prostate
Prostatic Neoplasms
Prostatic Hyperplasia
Neoplasms
Prostatitis
DNA
Immunohistochemistry
Odds Ratio
Polymerase Chain Reaction
Statistical Data Interpretation
DNA Sequence Analysis
Epidemiologic Studies

Keywords

  • Benign prostate hyperplasia
  • Helicobacter pylori infection
  • PCR
  • Prostate cancer

ASJC Scopus subject areas

  • Oncology
  • Reproductive Medicine
  • Urology

Cite this

Molecular evidence of helicobacter pylori infection in prostate tumors. / Al-Marhoon, Mohammed S.; Ouhtit, Allal; Al-Abri, Aisha O.; Venkiteswaran, Krishna P.; Al-Busaidi, Qassim; Mathew, Josephkunju; Al-Haddabi, Ibrahim; Shareef, Omar; Aquil, Shahid; Rahman, Khalid; Al-Hashmi, Intisar; Gupta, Ishita; Ganguly, Shyam S.

In: Current Urology, Vol. 8, 04.09.2015, p. 138-143.

Research output: Contribution to journalArticle

Al-Marhoon, MS, Ouhtit, A, Al-Abri, AO, Venkiteswaran, KP, Al-Busaidi, Q, Mathew, J, Al-Haddabi, I, Shareef, O, Aquil, S, Rahman, K, Al-Hashmi, I, Gupta, I & Ganguly, SS 2015, 'Molecular evidence of helicobacter pylori infection in prostate tumors', Current Urology, vol. 8, pp. 138-143. https://doi.org/10.1159/000365705
Al-Marhoon, Mohammed S. ; Ouhtit, Allal ; Al-Abri, Aisha O. ; Venkiteswaran, Krishna P. ; Al-Busaidi, Qassim ; Mathew, Josephkunju ; Al-Haddabi, Ibrahim ; Shareef, Omar ; Aquil, Shahid ; Rahman, Khalid ; Al-Hashmi, Intisar ; Gupta, Ishita ; Ganguly, Shyam S. / Molecular evidence of helicobacter pylori infection in prostate tumors. In: Current Urology. 2015 ; Vol. 8. pp. 138-143.
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AU - Ouhtit, Allal

AU - Al-Abri, Aisha O.

AU - Venkiteswaran, Krishna P.

AU - Al-Busaidi, Qassim

AU - Mathew, Josephkunju

AU - Al-Haddabi, Ibrahim

AU - Shareef, Omar

AU - Aquil, Shahid

AU - Rahman, Khalid

AU - Al-Hashmi, Intisar

AU - Gupta, Ishita

AU - Ganguly, Shyam S.

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N2 - Objectives: To determine whether Helicobacter pylori (H. pylori) is detectable in both benign prostatic hyperplasia (BPH) and prostate cancer (PCa). Epidemiological studies have shown significant associations between infective chronic prostatitis and prostatic carcinoma. Many bacteria have been found in the prostate of patients with chronic prostatitis, BPH, and PCa. Methods: One hundred consecutive patients with prostate diseases were enrolled in the study. Detection of H. pylori DNA in prostate tissue from patients with BPH and PCa was performed using both immunohistochemistry and PCR, and the results were confirmed by DNA sequencing. Odds ratios and the Fisher Exact test were used for the analysis of the associations between the variables. Results: Among the patients, 78% had BPH and 19% had PCa. While immunohistochemistry showed no positive sample for H. pylori, PCR combined with sequencing detected H. pylori DNA in prostate tissue samples from 5 patients. However, statistical analysis of the data showed that BPH and PCa are not significantly associated with the presence of H. pylori DNA in prostate tissue (odds ratio = 0.94, 95% confidence interval = 0.09-23.34, one-tailed Chi-square value = 0.660, p > 0.05). The limitation of this study was the small number of PCa patients. Conclusions: This study provides, for the first time, molecular evidence of the presence of H. pylori DNA in prostatic tissue of patients with BPH and PCa. It paves the way for further comprehensive studies to examine the association of H. pylori infection with BPH and PCa.

AB - Objectives: To determine whether Helicobacter pylori (H. pylori) is detectable in both benign prostatic hyperplasia (BPH) and prostate cancer (PCa). Epidemiological studies have shown significant associations between infective chronic prostatitis and prostatic carcinoma. Many bacteria have been found in the prostate of patients with chronic prostatitis, BPH, and PCa. Methods: One hundred consecutive patients with prostate diseases were enrolled in the study. Detection of H. pylori DNA in prostate tissue from patients with BPH and PCa was performed using both immunohistochemistry and PCR, and the results were confirmed by DNA sequencing. Odds ratios and the Fisher Exact test were used for the analysis of the associations between the variables. Results: Among the patients, 78% had BPH and 19% had PCa. While immunohistochemistry showed no positive sample for H. pylori, PCR combined with sequencing detected H. pylori DNA in prostate tissue samples from 5 patients. However, statistical analysis of the data showed that BPH and PCa are not significantly associated with the presence of H. pylori DNA in prostate tissue (odds ratio = 0.94, 95% confidence interval = 0.09-23.34, one-tailed Chi-square value = 0.660, p > 0.05). The limitation of this study was the small number of PCa patients. Conclusions: This study provides, for the first time, molecular evidence of the presence of H. pylori DNA in prostatic tissue of patients with BPH and PCa. It paves the way for further comprehensive studies to examine the association of H. pylori infection with BPH and PCa.

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