Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers

K. Bharati, S. S. Hasson, J. Oliver, G. D. Laing, R. D G Theakston, R. A. Harrison

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Venom toxin-specific antibodies offer a more rational treatment of snake envenoming than conventional antivenom. Here, we describe novel cDNAs encoding phospholipase A2 (PLA2) isoforms from venom gland RNA of Echis pyramidum leakeyi (Epl), Echis sochureki (Es) and Echis ocellatus (Eo). The deduced amino acid sequences of these cDNAs encoded proteins with high overall sequence identity to the viper group II PLA2 protein family, including the 14 cysteine residues capable of forming seven disulphide bonds that characterize this group of PLA2 enzymes. Comparison of the PLA2 sequences from Echis with those from related vipers failed to make significant geographic, taxonomic or PLA2-function distinctions between these Echis PLA2 isoforms. However, their deduced hydrophilicity profiles revealed a conserved tertiary structure that we will exploit, by epidermal DNA immunization, to generate PLA2- neutralizing antibodies with polyspecific potential.

Original languageEnglish
Pages (from-to)941-947
Number of pages7
JournalToxicon
Volume41
Issue number8
DOIs
Publication statusPublished - Jun 2003

Fingerprint

Cloning
Phospholipases A2
Venoms
Molecular Cloning
Protein Isoforms
Complementary DNA
Group II Phospholipases A2
Immunization
Antivenins
Snakes
Hydrophilicity
Neutralizing Antibodies
Hydrophobic and Hydrophilic Interactions
Disulfides
Cysteine
Amino Acid Sequence
Proteins
RNA
Amino Acids
Antibodies

Keywords

  • CDNA cloning
  • Echis ocellatus
  • Echis pyramidum leakeyi
  • Echis sochureki
  • Phospholipases A

ASJC Scopus subject areas

  • Toxicology

Cite this

Bharati, K., Hasson, S. S., Oliver, J., Laing, G. D., Theakston, R. D. G., & Harrison, R. A. (2003). Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers. Toxicon, 41(8), 941-947. https://doi.org/10.1016/S0041-0101(02)00312-4

Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers. / Bharati, K.; Hasson, S. S.; Oliver, J.; Laing, G. D.; Theakston, R. D G; Harrison, R. A.

In: Toxicon, Vol. 41, No. 8, 06.2003, p. 941-947.

Research output: Contribution to journalArticle

Bharati, K, Hasson, SS, Oliver, J, Laing, GD, Theakston, RDG & Harrison, RA 2003, 'Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers', Toxicon, vol. 41, no. 8, pp. 941-947. https://doi.org/10.1016/S0041-0101(02)00312-4
Bharati, K. ; Hasson, S. S. ; Oliver, J. ; Laing, G. D. ; Theakston, R. D G ; Harrison, R. A. / Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers. In: Toxicon. 2003 ; Vol. 41, No. 8. pp. 941-947.
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