Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers

K. Bharati, S. S. Hasson, J. Oliver, G. D. Laing, R. D G Theakston, R. A. Harrison

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Venom toxin-specific antibodies offer a more rational treatment of snake envenoming than conventional antivenom. Here, we describe novel cDNAs encoding phospholipase A2 (PLA2) isoforms from venom gland RNA of Echis pyramidum leakeyi (Epl), Echis sochureki (Es) and Echis ocellatus (Eo). The deduced amino acid sequences of these cDNAs encoded proteins with high overall sequence identity to the viper group II PLA2 protein family, including the 14 cysteine residues capable of forming seven disulphide bonds that characterize this group of PLA2 enzymes. Comparison of the PLA2 sequences from Echis with those from related vipers failed to make significant geographic, taxonomic or PLA2-function distinctions between these Echis PLA2 isoforms. However, their deduced hydrophilicity profiles revealed a conserved tertiary structure that we will exploit, by epidermal DNA immunization, to generate PLA2- neutralizing antibodies with polyspecific potential.

Original languageEnglish
Pages (from-to)941-947
Number of pages7
JournalToxicon
Volume41
Issue number8
DOIs
Publication statusPublished - Jun 2003

Keywords

  • CDNA cloning
  • Echis ocellatus
  • Echis pyramidum leakeyi
  • Echis sochureki
  • Phospholipases A

ASJC Scopus subject areas

  • Toxicology

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    Bharati, K., Hasson, S. S., Oliver, J., Laing, G. D., Theakston, R. D. G., & Harrison, R. A. (2003). Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers. Toxicon, 41(8), 941-947. https://doi.org/10.1016/S0041-0101(02)00312-4