Molecular characterization of a distinct monopartite begomovirus associated with betasatellites and alphasatellites infecting Pisum sativum in Nepal

M. S. Shahid, B. J. Pudashini, G. B. Khatri-Chhetri, R. W. Briddon, K. T. Natsuaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pea (Pisum sativum) plants exhibiting leaf distortion, yellowing, stunted growth and reduction in leaf size from Rampur, Nepal were shown to be infected by a begomovirus in association with betasatellites and alphasatellites. The begomovirus associated with the disease showed only low levels of nucleotide sequence identity (<91%) to previously characterized begomoviruses. This finding indicates that the pea samples were infected with an as yet undescribed begomovirus for which the name Pea leaf distortion virus (PLDV) is proposed. Two species of betasatellite were identified in association with PLDV. One group of sequences had high (>78%) nucleotide sequence identity to isolates of Ludwigia leaf distortion betasatellite (LuLDB), and the second group had less than 78% to all other betasatellite sequences. This showed PLDV to be associated with either LuLDB or a previously undescribed betasatellite for which the name Pea leaf distortion betasatellite is proposed. Two types of alphasatellites were identified in the PLDV-infected pea plants. The first type showed high levels of sequence identity to Ageratum yellow vein alphasatellite, and the second type showed high levels of identity to isolates of Sida yellow vein China alphasatellite. These are the first begomovirus, betasatellites and alphasatellites isolated from pea.

Original languageEnglish
Pages (from-to)300-306
Number of pages7
JournalVirus Genes
Volume53
Issue number2
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Begomovirus
Nepal
Peas
Onagraceae
Veins
Ageratum
Growth Disorders
Plant Leaves
Names
China

Keywords

  • Alphasatellite
  • Begomovirus
  • Betasatellite
  • Geminiviridae
  • Pisum sativum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Molecular characterization of a distinct monopartite begomovirus associated with betasatellites and alphasatellites infecting Pisum sativum in Nepal. / Shahid, M. S.; Pudashini, B. J.; Khatri-Chhetri, G. B.; Briddon, R. W.; Natsuaki, K. T.

In: Virus Genes, Vol. 53, No. 2, 01.04.2017, p. 300-306.

Research output: Contribution to journalArticle

Shahid, M. S. ; Pudashini, B. J. ; Khatri-Chhetri, G. B. ; Briddon, R. W. ; Natsuaki, K. T. / Molecular characterization of a distinct monopartite begomovirus associated with betasatellites and alphasatellites infecting Pisum sativum in Nepal. In: Virus Genes. 2017 ; Vol. 53, No. 2. pp. 300-306.
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