TY - JOUR
T1 - Molecular characterization and phylogenetic analysis of human pathogenic Leptospira species circulating in a tertiary care hospital of Western Uttar Pradesh in India
AU - Mahtab, Md
AU - Khan, Fatima
AU - Azam, Mohd
AU - Rizvi, Meher
AU - Sultan, Asfia
AU - Shukla, Indu
AU - Almatroudi, Ahmad
N1 - Funding Information:
Authors wish to thank, Dr. Vijayachari P, Dr. Vimal Raj and Mr. Vinod, Regional Centre for Leptospirosis, ICMR, Port Blair, for providing training and other technical support. The authors are thankful to DST-SERB and UGC for financial help.
Funding Information:
This work was supported by the SERB, Department of Science and Technology [SB/YS/LS-96/2013]. Authors wish to thank, Dr. Vijayachari P, Dr. Vimal Raj and Mr. Vinod, Regional Centre for Leptospirosis, ICMR, Port Blair, for providing training and other technical support. The authors are thankful to DST-SERB and UGC for financial help.
Publisher Copyright:
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2019/8/18
Y1 - 2019/8/18
N2 - Leptospirosis is identified as an important reemerging zoonotic disease distributed worldwide, caused by Leptospira. This study was carried out to explore the genetic characterization and its phylogenetic analysis of circulating Leptospira species, among the Aligarh region of western Uttar Pradesh in India, utilizing secY gene-based nucleotide sequence. A total of 190 human samples were included in the study. Positive samples were identified by ELISA, MAT and PCR. MAT was carried out utilizing local circulating Leptospira serovars. Four positive samples including two MAT positive samples were subjected to DNA sequencing for further confirmation and phylogenetic tree was constructed. Out of the total of 190 samples, 24 patients were found positive by ELISA and 29 by PCR. Two samples were found reactive in MAT with L. interrogans serovars like hebdomadis and copenhageni. Phylogenetic analysis of four isolates based on partial secY gene nucleotide sequences revealed that species obtained from the Aligarh region clustered with the several published pathogenic Leptospira interrogans, while some of our isolates nucleotide sequences also clustered with the published sequence of intermediate and saprophytic Leptospira serovars like Leptospira inadai and Leptospira meyeri. This pilot study will help us to decipher the present scenario of circulating serovars of leptospira as well as to identify the nucleotide changes in secY gene, in this region.
AB - Leptospirosis is identified as an important reemerging zoonotic disease distributed worldwide, caused by Leptospira. This study was carried out to explore the genetic characterization and its phylogenetic analysis of circulating Leptospira species, among the Aligarh region of western Uttar Pradesh in India, utilizing secY gene-based nucleotide sequence. A total of 190 human samples were included in the study. Positive samples were identified by ELISA, MAT and PCR. MAT was carried out utilizing local circulating Leptospira serovars. Four positive samples including two MAT positive samples were subjected to DNA sequencing for further confirmation and phylogenetic tree was constructed. Out of the total of 190 samples, 24 patients were found positive by ELISA and 29 by PCR. Two samples were found reactive in MAT with L. interrogans serovars like hebdomadis and copenhageni. Phylogenetic analysis of four isolates based on partial secY gene nucleotide sequences revealed that species obtained from the Aligarh region clustered with the several published pathogenic Leptospira interrogans, while some of our isolates nucleotide sequences also clustered with the published sequence of intermediate and saprophytic Leptospira serovars like Leptospira inadai and Leptospira meyeri. This pilot study will help us to decipher the present scenario of circulating serovars of leptospira as well as to identify the nucleotide changes in secY gene, in this region.
KW - Aligarh
KW - Leptospirosis
KW - phylogenetic analysis
KW - sequencing
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U2 - 10.1080/20477724.2019.1685196
DO - 10.1080/20477724.2019.1685196
M3 - Article
C2 - 31818236
AN - SCOPUS:85076381291
SN - 2047-7724
VL - 113
SP - 275
EP - 281
JO - Annals of Tropical Medicine and Parasitology
JF - Annals of Tropical Medicine and Parasitology
IS - 6
ER -