Molecular and biological characterization of Chilli leaf curl virus and associated betasatellite infecting Cucurbita maxima in Oman

M. S. Shahid*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

During a survey in February 2016, leaf curl disease symptoms were witnessed in Cucurbita maxima plants in Al-Batina commercial farm in Oman. Symptoms exhibited were characteristic of begomovirus infection as leaf curling, yellowing, and color breaking followed by mosaic pattern. The transmission electron microscopy confirmed the presence of typical twinned geminate typical of Geminate virus particles. Rolling circle amplification (RCA) was employed to characterize the unknown causal agent of C. maxima disease. In molecular identification RCA produced nearly 2.8 and 1.4 kb DNA molecules corresponding to begomovirus and satellite molecules, cloned and sequenced them. In Blast, species demarcation tool and phylogenetic analysis revealed the begomovirus and satellite isolates were determined as Chilli leaf curl virus (ChLCV) and tomato leaf curl betasatellite (ToLCB). In biological analysis by agrobacterium mediated inoculation, ChLCV displayed upward leaf curling and vein swelling symptoms in Nicotiana benthamiana plants; however, in presence of ToLCB enhanced downward leaf curling and crumpling symptoms were revealed. This study provides the first evidence that ChLCV and ToLCB caused leaf curl disease of C. maxima in Oman.

Original languageEnglish
Pages (from-to)378-382
Number of pages5
JournalVirusDisease
Volume31
Issue number3
DOIs
Publication statusPublished - Sep 1 2020

Keywords

  • Begomovirus
  • Chilli leaf curl virus
  • Cucurbita maxima
  • Geminiviridae
  • Tomato leaf curl betasatellite

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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