Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon

L. C. Ranford-Cartwright; J. Taylor; T. Umasunthar; L. H. Taylor; H. A. Babiker; B. Lell; J. R. Schmidt-Ott; L. G. Lehman; D. Walliker; P. G. Kremsner

doi:10.1016/S0035-9203(97)90539-3

Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon

L. C. Ranford-Cartwright^*, J. Taylor, T. Umasunthar, L. H. Taylor, H. A. Babiker, B. Lell, J. R. Schmidt-Ott, L. G. Lehman, D. Walliker, P. G. Kremsner

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.

Original language	English
Pages (from-to)	719-724
Number of pages	6
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	91
Issue number	6
DOIs	https://doi.org/10.1016/S0035-9203(97)90539-3
Publication status	Published - 1997
Externally published	Yes

Keywords

Drug resistance
Genotypes
Malaria
Plasmodium falciparum
Polymerase chain reaction
Recrudescence

ASJC Scopus subject areas

Parasitology
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/S0035-9203(97)90539-3

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Ranford-Cartwright, L. C., Taylor, J., Umasunthar, T., Taylor, L. H., Babiker, H. A., Lell, B., Schmidt-Ott, J. R., Lehman, L. G., Walliker, D., & Kremsner, P. G. (1997). Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon. Transactions of the Royal Society of Tropical Medicine and Hygiene, 91(6), 719-724. https://doi.org/10.1016/S0035-9203(97)90539-3

Ranford-Cartwright, LC, Taylor, J, Umasunthar, T, Taylor, LH, Babiker, HA, Lell, B, Schmidt-Ott, JR, Lehman, LG, Walliker, D & Kremsner, PG 1997, 'Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 91, no. 6, pp. 719-724. https://doi.org/10.1016/S0035-9203(97)90539-3

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title = "Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon",

abstract = "Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.",

keywords = "Drug resistance, Genotypes, Malaria, Plasmodium falciparum, Polymerase chain reaction, Recrudescence",

author = "Ranford-Cartwright, {L. C.} and J. Taylor and T. Umasunthar and Taylor, {L. H.} and Babiker, {H. A.} and B. Lell and Schmidt-Ott, {J. R.} and Lehman, {L. G.} and D. Walliker and Kremsner, {P. G.}",

note = "Funding Information: Acknowledgements We thank Jurg Handschin, of F. Hoffman La Roche, for the opportunity to carry out this study. The Edinburgh group is supported by the Medical Research Council. This analysis was sponsored by F. Hoffman-La Roche, Basel, Switzerland.",

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Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon

Abstract

Keywords

ASJC Scopus subject areas

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