Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon

L. C. Ranford-Cartwright, J. Taylor, T. Umasunthar, L. H. Taylor, H. A. Babiker, B. Lell, J. R. Schmidt-Ott, L. G. Lehman, D. Walliker, P. G. Kremsner

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Abstract

Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.

Original languageEnglish
Pages (from-to)719-724
Number of pages6
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume91
Issue number6
DOIs
Publication statusPublished - 1997

Fingerprint

Gabon
Plasmodium falciparum
Parasites
Pharmaceutical Preparations
Therapeutics
Merozoite Surface Protein 1
Recurrence
Population Growth
Culicidae
Fingers

Keywords

  • Drug resistance
  • Genotypes
  • Malaria
  • Plasmodium falciparum
  • Polymerase chain reaction
  • Recrudescence

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)

Cite this

Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon. / Ranford-Cartwright, L. C.; Taylor, J.; Umasunthar, T.; Taylor, L. H.; Babiker, H. A.; Lell, B.; Schmidt-Ott, J. R.; Lehman, L. G.; Walliker, D.; Kremsner, P. G.

In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 91, No. 6, 1997, p. 719-724.

Research output: Contribution to journalArticle

Ranford-Cartwright, LC, Taylor, J, Umasunthar, T, Taylor, LH, Babiker, HA, Lell, B, Schmidt-Ott, JR, Lehman, LG, Walliker, D & Kremsner, PG 1997, 'Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 91, no. 6, pp. 719-724. https://doi.org/10.1016/S0035-9203(97)90539-3
Ranford-Cartwright, L. C. ; Taylor, J. ; Umasunthar, T. ; Taylor, L. H. ; Babiker, H. A. ; Lell, B. ; Schmidt-Ott, J. R. ; Lehman, L. G. ; Walliker, D. ; Kremsner, P. G. / Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon. In: Transactions of the Royal Society of Tropical Medicine and Hygiene. 1997 ; Vol. 91, No. 6. pp. 719-724.
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