TY - JOUR
T1 - Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon
AU - Ranford-Cartwright, L. C.
AU - Taylor, J.
AU - Umasunthar, T.
AU - Taylor, L. H.
AU - Babiker, H. A.
AU - Lell, B.
AU - Schmidt-Ott, J. R.
AU - Lehman, L. G.
AU - Walliker, D.
AU - Kremsner, P. G.
PY - 1997
Y1 - 1997
N2 - Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.
AB - Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.
KW - Drug resistance
KW - Genotypes
KW - Malaria
KW - Plasmodium falciparum
KW - Polymerase chain reaction
KW - Recrudescence
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U2 - 10.1016/S0035-9203(97)90539-3
DO - 10.1016/S0035-9203(97)90539-3
M3 - Article
C2 - 9509189
AN - SCOPUS:13144250200
VL - 91
SP - 719
EP - 724
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
SN - 0035-9203
IS - 6
ER -