Abstract
Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.
| Original language | English |
|---|---|
| Pages (from-to) | 719-724 |
| Number of pages | 6 |
| Journal | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| Volume | 91 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1997 |
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Keywords
- Drug resistance
- Genotypes
- Malaria
- Plasmodium falciparum
- Polymerase chain reaction
- Recrudescence
ASJC Scopus subject areas
- Parasitology
- Medicine(all)
Cite this
Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon. / Ranford-Cartwright, L. C.; Taylor, J.; Umasunthar, T.; Taylor, L. H.; Babiker, H. A.; Lell, B.; Schmidt-Ott, J. R.; Lehman, L. G.; Walliker, D.; Kremsner, P. G.
In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 91, No. 6, 1997, p. 719-724.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular analysis of recrudescent parasites in a Plasmodium falciparum drug efficacy trial in Gabon
AU - Ranford-Cartwright, L. C.
AU - Taylor, J.
AU - Umasunthar, T.
AU - Taylor, L. H.
AU - Babiker, H. A.
AU - Lell, B.
AU - Schmidt-Ott, J. R.
AU - Lehman, L. G.
AU - Walliker, D.
AU - Kremsner, P. G.
PY - 1997
Y1 - 1997
N2 - Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.
AB - Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.
KW - Drug resistance
KW - Genotypes
KW - Malaria
KW - Plasmodium falciparum
KW - Polymerase chain reaction
KW - Recrudescence
UR - http://www.scopus.com/inward/record.url?scp=13144250200&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13144250200&partnerID=8YFLogxK
U2 - 10.1016/S0035-9203(97)90539-3
DO - 10.1016/S0035-9203(97)90539-3
M3 - Article
VL - 91
SP - 719
EP - 724
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
SN - 0035-9203
IS - 6
ER -