Modeling and experiment yields the structure of acireductone dioxygenase from Klebsiella pneumoniae

Thomas C. Pochapsky, Susan Sondej Pochapsky, Tingting Ju, Huaping Mo, Faizah Al-Mjeni, Michael J. Maroney

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Here we report the structure of acireductone dioxygenase (ARD), the first determined for a new family of metalloenzymes. ARD represents a branch point in the methionine salvage pathway leading from methylthioadenosine to methionine and has been shown to catalyze different reactions depending on the type of metal ion bound in the active site. The solution structure of nickel-containing ARD (Ni-ARD) was determined using NMR methods. x-ray absorption spectroscopy, assignment of hyperfine shifted NMR resonances and conserved domain homology were used to model the metal-binding site because of the paramagnetism of the bound Ni2+. Although there is no structure in the Protein Data Bank within 3 Å r.m.s deviation of that of Ni-ARD, the enzyme active site is located in a conserved double-stranded β-helix domain. Furthermore, the proposed Ni-ARD active site shows significant post-facto structural homology to the active sites of several metalloenzymes in the cupin superfamily.

Original languageEnglish
Pages (from-to)966-972
Number of pages7
JournalNature Structural Biology
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

Klebsiella pneumoniae
Nickel
Catalytic Domain
Methionine
Nuclear magnetic resonance
Paramagnetism
Salvaging
Metals
Experiments
Absorption spectroscopy
Metal ions
Binding Sites
X rays
Spectrum Analysis
X-Rays
Databases
Enzymes
Ions
aci-reductone oxidase (CO-forming)
Proteins

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

Cite this

Modeling and experiment yields the structure of acireductone dioxygenase from Klebsiella pneumoniae. / Pochapsky, Thomas C.; Sondej Pochapsky, Susan; Ju, Tingting; Mo, Huaping; Al-Mjeni, Faizah; Maroney, Michael J.

In: Nature Structural Biology, Vol. 9, No. 12, 01.12.2002, p. 966-972.

Research output: Contribution to journalArticle

Pochapsky, Thomas C. ; Sondej Pochapsky, Susan ; Ju, Tingting ; Mo, Huaping ; Al-Mjeni, Faizah ; Maroney, Michael J. / Modeling and experiment yields the structure of acireductone dioxygenase from Klebsiella pneumoniae. In: Nature Structural Biology. 2002 ; Vol. 9, No. 12. pp. 966-972.
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