Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: A comparative serological study

Chantal B E M Reusken, Bart L. Haagmans, Marcel A. Müller, Carlos Gutierrez, Gert Jan Godeke, Benjamin Meyer, Doreen Muth, V. Stalin Raj, Laura Smits De Vries, Victor M. Corman, Jan Felix Drexler, Saskia L. Smits, Yasmin E. El Tahir, Rita De Sousa, Janko van Beek, Norbert Nowotny, Kees van Maanen, Ezequiel Hidalgo-Hermoso, Berend Jan Bosch, Peter RottierAlbert Osterhaus, Christian Gortázar-Schmidt, Christian Drosten, Marion P G Koopmans

Research output: Contribution to journalArticle

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Abstract

Background: A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods: We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings: 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation: MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding: European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.

Original languageEnglish
Pages (from-to)859-866
Number of pages8
JournalThe Lancet Infectious Diseases
Volume13
Issue number10
DOIs
Publication statusPublished - Oct 2013

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Camelus
Neutralizing Antibodies
Serum
Bovine Coronavirus
Goats
Oman
Antibodies
Viruses
Human Coronavirus OC43
Sheep
Middle East Respiratory Syndrome Coronavirus
Severe Acute Respiratory Syndrome
Neutralization Tests
Protein Array Analysis
Coronavirus
Middle East
Chile
Seroepidemiologic Studies
Protein Subunits
Livestock

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Reusken, C. B. E. M., Haagmans, B. L., Müller, M. A., Gutierrez, C., Godeke, G. J., Meyer, B., ... Koopmans, M. P. G. (2013). Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: A comparative serological study. The Lancet Infectious Diseases, 13(10), 859-866. https://doi.org/10.1016/S1473-3099(13)70164-6

Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels : A comparative serological study. / Reusken, Chantal B E M; Haagmans, Bart L.; Müller, Marcel A.; Gutierrez, Carlos; Godeke, Gert Jan; Meyer, Benjamin; Muth, Doreen; Raj, V. Stalin; Vries, Laura Smits De; Corman, Victor M.; Drexler, Jan Felix; Smits, Saskia L.; El Tahir, Yasmin E.; De Sousa, Rita; van Beek, Janko; Nowotny, Norbert; van Maanen, Kees; Hidalgo-Hermoso, Ezequiel; Bosch, Berend Jan; Rottier, Peter; Osterhaus, Albert; Gortázar-Schmidt, Christian; Drosten, Christian; Koopmans, Marion P G.

In: The Lancet Infectious Diseases, Vol. 13, No. 10, 10.2013, p. 859-866.

Research output: Contribution to journalArticle

Reusken, CBEM, Haagmans, BL, Müller, MA, Gutierrez, C, Godeke, GJ, Meyer, B, Muth, D, Raj, VS, Vries, LSD, Corman, VM, Drexler, JF, Smits, SL, El Tahir, YE, De Sousa, R, van Beek, J, Nowotny, N, van Maanen, K, Hidalgo-Hermoso, E, Bosch, BJ, Rottier, P, Osterhaus, A, Gortázar-Schmidt, C, Drosten, C & Koopmans, MPG 2013, 'Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: A comparative serological study', The Lancet Infectious Diseases, vol. 13, no. 10, pp. 859-866. https://doi.org/10.1016/S1473-3099(13)70164-6
Reusken, Chantal B E M ; Haagmans, Bart L. ; Müller, Marcel A. ; Gutierrez, Carlos ; Godeke, Gert Jan ; Meyer, Benjamin ; Muth, Doreen ; Raj, V. Stalin ; Vries, Laura Smits De ; Corman, Victor M. ; Drexler, Jan Felix ; Smits, Saskia L. ; El Tahir, Yasmin E. ; De Sousa, Rita ; van Beek, Janko ; Nowotny, Norbert ; van Maanen, Kees ; Hidalgo-Hermoso, Ezequiel ; Bosch, Berend Jan ; Rottier, Peter ; Osterhaus, Albert ; Gortázar-Schmidt, Christian ; Drosten, Christian ; Koopmans, Marion P G. / Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels : A comparative serological study. In: The Lancet Infectious Diseases. 2013 ; Vol. 13, No. 10. pp. 859-866.
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abstract = "Background: A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods: We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings: 50 of 50 (100{\%}) sera from Omani camels and 15 of 105 (14{\%}) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation: MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding: European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.",
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T2 - A comparative serological study

AU - Reusken, Chantal B E M

AU - Haagmans, Bart L.

AU - Müller, Marcel A.

AU - Gutierrez, Carlos

AU - Godeke, Gert Jan

AU - Meyer, Benjamin

AU - Muth, Doreen

AU - Raj, V. Stalin

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AU - Corman, Victor M.

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N2 - Background: A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. Methods: We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. Findings: 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. Interpretation: MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. Funding: European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.

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