Microsatellite DNA marker analysis in brown mussels, Perna perna (Linnaeus, 1758) from the coastal waters of Oman

Thirteen highly polymorphic microsatellite primer pairs developed for Perna uiridis from the gene bank were tested on 3 populations of Perna perna samples with the aim to adapt a fast, reliable method for preliminary screening and to genetically characterize the wild populations of P. perna in the Oman waters. The samples were collected from three different locations viz Ras Al-Had, Ras Madrakah and Mirbat. All primers showed high level of polymorphism for all populations. The mean observed heterozygosity was lower than the expected heterozygosity across the three populations which means there was a probability of inbreeding occurred in the populations. Both the chi-square (x²) and likelihood (G²) ratio tests detected significant differences (p