Microfluidic precolumn derivatization of environmental phenols with coumarin-6-sulfonyl chloride and HPLC separation

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Abstract

A simple, fast, sensitive and versatile method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (C6SCl) and HPLC separation on monolithic columns. Phenols react with C6SCl within 3.0 min in the microreactor at ambient temperature to produce phenol-coumarin sulphonamides derivatives which were separated in reversed phase high-performance liquid chromatography followed by postcolumn ring-opening and fluorescence detection at lexc 5 360 nm and lem 5 460 nm. The optimum conditions for the derivatization, separation and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of 0.75-12.5 mg L21. The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds.

Original languageEnglish
Pages (from-to)1379-1385
Number of pages7
JournalJournal of Chromatographic Science
Volume53
Issue number8
DOIs
Publication statusPublished - 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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