Microfluidic precolumn derivatization of environmental phenols with coumarin-6-sulfonyl chloride and HPLC separation

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Abstract

A simple, fast, sensitive and versatile method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (C6SCl) and HPLC separation on monolithic columns. Phenols react with C6SCl within 3.0 min in the microreactor at ambient temperature to produce phenol-coumarin sulphonamides derivatives which were separated in reversed phase high-performance liquid chromatography followed by postcolumn ring-opening and fluorescence detection at lexc 5 360 nm and lem 5 460 nm. The optimum conditions for the derivatization, separation and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of 0.75-12.5 mg L21. The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds.

Original languageEnglish
Pages (from-to)1379-1385
Number of pages7
JournalJournal of Chromatographic Science
Volume53
Issue number8
DOIs
Publication statusPublished - 2015

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Phenols
Microfluidics
Fountains
Water
Sulfonamides
High performance liquid chromatography
Phenol
Labels
Fluorescence
Calibration
Derivatives
coumarin-6-sulfonyl chloride
Temperature

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Microfluidic precolumn derivatization of environmental phenols with coumarin-6-sulfonyl chloride and HPLC separation",
abstract = "A simple, fast, sensitive and versatile method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (C6SCl) and HPLC separation on monolithic columns. Phenols react with C6SCl within 3.0 min in the microreactor at ambient temperature to produce phenol-coumarin sulphonamides derivatives which were separated in reversed phase high-performance liquid chromatography followed by postcolumn ring-opening and fluorescence detection at lexc 5 360 nm and lem 5 460 nm. The optimum conditions for the derivatization, separation and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of 0.75-12.5 mg L21. The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds.",
author = "Suliman, {Fakhreldin O.} and Al-Busaidi, {Jihad N.} and Al-Lawati, {Hiader A J} and Al-Kindy, {Salma M Z}",
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T1 - Microfluidic precolumn derivatization of environmental phenols with coumarin-6-sulfonyl chloride and HPLC separation

AU - Suliman, Fakhreldin O.

AU - Al-Busaidi, Jihad N.

AU - Al-Lawati, Hiader A J

AU - Al-Kindy, Salma M Z

PY - 2015

Y1 - 2015

N2 - A simple, fast, sensitive and versatile method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (C6SCl) and HPLC separation on monolithic columns. Phenols react with C6SCl within 3.0 min in the microreactor at ambient temperature to produce phenol-coumarin sulphonamides derivatives which were separated in reversed phase high-performance liquid chromatography followed by postcolumn ring-opening and fluorescence detection at lexc 5 360 nm and lem 5 460 nm. The optimum conditions for the derivatization, separation and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of 0.75-12.5 mg L21. The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds.

AB - A simple, fast, sensitive and versatile method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (C6SCl) and HPLC separation on monolithic columns. Phenols react with C6SCl within 3.0 min in the microreactor at ambient temperature to produce phenol-coumarin sulphonamides derivatives which were separated in reversed phase high-performance liquid chromatography followed by postcolumn ring-opening and fluorescence detection at lexc 5 360 nm and lem 5 460 nm. The optimum conditions for the derivatization, separation and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of 0.75-12.5 mg L21. The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds.

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