Metabolomic characterization of italian sweet pepper (Capsicum annum L.) by means of HRMAS-NMR spectroscopy and multivariate analysis

Mena Ritota, Federico Marini, Paolo Sequi, Massimiliano Valentini

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

HRMAS-NMR spectroscopy was used to assess the metabolic profile of sweet pepper (Capsicum Annum L.). One-dimensional and two-dimensional NMR spectra, performed directly on sample pieces of few milligrams, hence without any chemical and/or physical manipulation, allowed the assignment of several compounds. Organic acids, fatty acids, amino acids, and minor compounds such as trigonelline, C4-substituted pyridine, choline, and cinnamic derivatives were observed with a single experiment. A significant discrimination between the two sweet pepper varieties was found by using partial least-squares projections to latent structures discrimination analysis (PLS-DA). The metabolites contributing predominantly to such differentiation were sugars and organic and fatty acids. Also a partial separation according to the geographical origin was obtained always by analyzing the NMR data with PLS-DA. Some of the discriminating molecules are peculiar for pepper and contribute to define the overall commercial and organoleptic quality so that HRMAS-NMR proved to be a complementary analysis to standard tools used in food science and, in principle, can be applied to any foodstuff.

Original languageEnglish
Pages (from-to)9675-9684
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number17
DOIs
Publication statusPublished - Sep 8 2010

Fingerprint

Capsicum
Metabolomics
metabolomics
sweet peppers
Nuclear magnetic resonance spectroscopy
multivariate analysis
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Multivariate Analysis
Organic acids
Nuclear magnetic resonance
organic acids and salts
Fatty Acids
trigonelline
fatty acids
Sugar Acids
Food Technology
Metabolome
pyridines
choline

Keywords

  • cultivar
  • HRMAS-NMR
  • metabolomics
  • PLS-DA
  • Sweet pepper
  • traceability

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Metabolomic characterization of italian sweet pepper (Capsicum annum L.) by means of HRMAS-NMR spectroscopy and multivariate analysis. / Ritota, Mena; Marini, Federico; Sequi, Paolo; Valentini, Massimiliano.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 17, 08.09.2010, p. 9675-9684.

Research output: Contribution to journalArticle

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