Metabolic response of downy mildew resistant and susceptible maize genotypes to Peronosclerospora sorghi

Y. Sireesha, Meena Sekhar, R. Velazhahan

Research output: Contribution to journalArticle

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Abstract

The metabolic response of downy mildew resistant (MAI 756) and susceptible (CM 500) maize (Zea mays L.) genotypes to Peronosclerospora sorghi (Weston & Uppal) Shaw, the causal fungus of maize downy mildew was assessed 0, 24, 48 and 72 h after inoculation by using gas chromatography coupled to electron impact ionization-time of flight-mass spectrometry (GC/EI-TOF-MS). A total of 95 metabolites were identified and annotated using the Golm Metabolome Database and Tag Finder software. Among them, 19 metabolites were up-regulated and unique to MAI 756, 4 up-regulated metabolites were unique to CM 500 and 72 metabolites were common to both the genotypes. Infection by P. sorghi substantially alters the plant metabolic profile, including significant changes in sugars, amino acids and phenylpropanoids. Up-regulation of DL-ornithine, cis-aconitic acid, citric acid, phthalic acid, cellobiose and xylopyranose were detected in the resistant maize genotype 24 h after inoculation, whereas N-acetyl-L-lysine, L-threonic acid, 1-cyclohexene-1-carboxylic acid and cinnamic acid have been detected 48 h after inoculation. The metabolites viz., erythro-pentonic acid and octadecenoic acid were up-regulated in the susceptible genotype 48 h after inoculation. This investigation provides new insight into the mechanisms of resistance in maize to P. sorghi at the metabolite level.

Original languageEnglish
Pages (from-to)1355-1362
Number of pages8
JournalJournal of Pure and Applied Microbiology
Volume9
Issue number2
Publication statusPublished - Jun 1 2015

Fingerprint

Zea mays
Genotype
NSC 153174
Metabolome
Aconitic Acid
Cellobiose
Sugar Acids
Ornithine
Carboxylic Acids
Citric Acid
Gas Chromatography
Lysine
Mass Spectrometry
Fungi
Up-Regulation
Software
Databases
Electrons
Amino Acids
Acids

Keywords

  • Downy mildew
  • Maize
  • Metabolomics
  • Peronosclerospora sorghi
  • Zea mays

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Metabolic response of downy mildew resistant and susceptible maize genotypes to Peronosclerospora sorghi. / Sireesha, Y.; Sekhar, Meena; Velazhahan, R.

In: Journal of Pure and Applied Microbiology, Vol. 9, No. 2, 01.06.2015, p. 1355-1362.

Research output: Contribution to journalArticle

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