Abstract
A lot of research has been performed on Cyanobacteria and microalgae with the aim to produce numerous biotechnological products. However, native strains have a few shortcomings, like limitations in cultivation, harvesting and product extraction, which prevents reaching optimal production value at lowest costs. Such limitations require the intervention of genetic engineering to produce strains with superior properties. Promising advancements in the cultivation of Cyanobacteria and microalgae have been achieved by improving photosynthetic efficiency through increasing RuBisCO activity and truncation of light-harvesting antennae. Genetic engineering has also contributed to final product extraction by inducing autolysis and product secretory systems, to enable direct product recovery without going through costly extraction steps. In this review, we summarize the different enzymes and pathways that have been targeted thus far for improving cultivation aspects, harvesting and product extraction in Cyanobacteria and microalgae. With synthetic biology advancements, genetically engineered strains can be generated to resolve demanding process issues and achieve economic practicality. This comprehensive overview of gene modifications will be useful to researchers in the field to employ on their strains to increase their yields and improve the economic feasibility of the production process.
| Original language | English |
|---|---|
| Pages (from-to) | 919-931 |
| Number of pages | 13 |
| Journal | Journal of Applied Microbiology |
| Volume | 121 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 1 2016 |
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Keywords
- algae
- biofuels
- Cyanobacteria
- high-value
- metabolic engineering
- production
ASJC Scopus subject areas
- Biotechnology
- Medicine(all)
- Applied Microbiology and Biotechnology
Cite this
Metabolic engineering of Cyanobacteria and microalgae for enhanced production of biofuels and high-value products. / Gomaa, M. A.; Al-Haj, L.; Abed, R. M M.
In: Journal of Applied Microbiology, Vol. 121, No. 4, 01.10.2016, p. 919-931.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Metabolic engineering of Cyanobacteria and microalgae for enhanced production of biofuels and high-value products
AU - Gomaa, M. A.
AU - Al-Haj, L.
AU - Abed, R. M M
PY - 2016/10/1
Y1 - 2016/10/1
N2 - A lot of research has been performed on Cyanobacteria and microalgae with the aim to produce numerous biotechnological products. However, native strains have a few shortcomings, like limitations in cultivation, harvesting and product extraction, which prevents reaching optimal production value at lowest costs. Such limitations require the intervention of genetic engineering to produce strains with superior properties. Promising advancements in the cultivation of Cyanobacteria and microalgae have been achieved by improving photosynthetic efficiency through increasing RuBisCO activity and truncation of light-harvesting antennae. Genetic engineering has also contributed to final product extraction by inducing autolysis and product secretory systems, to enable direct product recovery without going through costly extraction steps. In this review, we summarize the different enzymes and pathways that have been targeted thus far for improving cultivation aspects, harvesting and product extraction in Cyanobacteria and microalgae. With synthetic biology advancements, genetically engineered strains can be generated to resolve demanding process issues and achieve economic practicality. This comprehensive overview of gene modifications will be useful to researchers in the field to employ on their strains to increase their yields and improve the economic feasibility of the production process.
AB - A lot of research has been performed on Cyanobacteria and microalgae with the aim to produce numerous biotechnological products. However, native strains have a few shortcomings, like limitations in cultivation, harvesting and product extraction, which prevents reaching optimal production value at lowest costs. Such limitations require the intervention of genetic engineering to produce strains with superior properties. Promising advancements in the cultivation of Cyanobacteria and microalgae have been achieved by improving photosynthetic efficiency through increasing RuBisCO activity and truncation of light-harvesting antennae. Genetic engineering has also contributed to final product extraction by inducing autolysis and product secretory systems, to enable direct product recovery without going through costly extraction steps. In this review, we summarize the different enzymes and pathways that have been targeted thus far for improving cultivation aspects, harvesting and product extraction in Cyanobacteria and microalgae. With synthetic biology advancements, genetically engineered strains can be generated to resolve demanding process issues and achieve economic practicality. This comprehensive overview of gene modifications will be useful to researchers in the field to employ on their strains to increase their yields and improve the economic feasibility of the production process.
KW - algae
KW - biofuels
KW - Cyanobacteria
KW - high-value
KW - metabolic engineering
KW - production
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UR - http://www.scopus.com/inward/citedby.url?scp=84987643663&partnerID=8YFLogxK
U2 - 10.1111/jam.13232
DO - 10.1111/jam.13232
M3 - Review article
C2 - 27406848
AN - SCOPUS:84987643663
VL - 121
SP - 919
EP - 931
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
SN - 1364-5072
IS - 4
ER -