Mechanisms of vasorelaxation induced by oleoylethanolamide in the rat small mesenteric artery

Yousuf M. Alsuleimani, C. Robin Hiley

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The actions of the anandamide-like mono-unsaturated fatty acid oleoylethanolamide (OEA) were first linked to satiety and control of food intake and recently reported to relax resistance vessels. This study characterizes its vasorelaxant mechanisms. Vasorelaxation to OEA were assessed in third order branches of rat superior mesenteric artery using a wire myograph. The roles of the endothelium, KCa channels, perivascular sensory nerves, NO, cannabinoid receptors, and the phospholipase C (PLC)/inositol trisphosphate (InsP3) and RhoA/ROCK signalling pathways, were assessed. OEA caused concentration- and endothelium-dependent vasorelaxation (pEC50=6.7±0.1, Rmax=93.1±2. 5%). L-NAME greatly reduced the response (residual relaxation of only 24.6±12.8%). Capsaicin and pertussis toxin significantly reduced the vasorelaxation. Precontraction with KCl abolished the response. TRAM-34 had no effect, but both iberiotoxin and apamin+charybdotoxin markedly shifted the OEA concentration-response curve to the right (∼5-fold). O-1918 but not rimonabant attenuated the vasorelaxation. Both the CB1 receptor antagonist, AM251 and the CB2 receptor antagonist, AM630, given alone or in combination, reduced the response to OEA. Inhibition of PLC by U73122, ROCK by Y-27632 and antagonism of inositol trisphosphate (InsP3) receptors by 2-APB abolished OEA vasorelaxation. OEA vasorelaxation involves an endothelial site of action but not the known cannabinoid receptors. It involves Ca 2+ released from InsP3-sensitive endothelial stores by mechanisms involving RhoA kinase and phospholipase C. It is likely that the released Ca2+ causes NO generation and opening of mainly large-conductance KCa channels. This study demonstrates a possible novel endothelial target that might be important in the control of regional blood flow induced by this lipid molecule. Crown

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalEuropean Journal of Pharmacology
Volume702
Issue number1-3
DOIs
Publication statusPublished - Feb 28 2013

Fingerprint

Mesenteric Arteries
Vasodilation
Type C Phospholipases
Cannabinoid Receptors
rimonabant
Inositol
Endothelium
Cannabinoid Receptor CB2
Charybdotoxin
Apamin
Cannabinoid Receptor CB1
Superior Mesenteric Artery
Capsaicin
NG-Nitroarginine Methyl Ester
Pertussis Toxin
Regional Blood Flow
oleoylethanolamide
Crowns
Unsaturated Fatty Acids
Vasodilator Agents

Keywords

  • Ca-activated K channels
  • Cannabinoid receptors
  • Nitric oxide
  • Oleoylethanolamide
  • Phospholipase C
  • Vasorelaxation

ASJC Scopus subject areas

  • Pharmacology

Cite this

Mechanisms of vasorelaxation induced by oleoylethanolamide in the rat small mesenteric artery. / Alsuleimani, Yousuf M.; Hiley, C. Robin.

In: European Journal of Pharmacology, Vol. 702, No. 1-3, 28.02.2013, p. 1-11.

Research output: Contribution to journalArticle

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abstract = "The actions of the anandamide-like mono-unsaturated fatty acid oleoylethanolamide (OEA) were first linked to satiety and control of food intake and recently reported to relax resistance vessels. This study characterizes its vasorelaxant mechanisms. Vasorelaxation to OEA were assessed in third order branches of rat superior mesenteric artery using a wire myograph. The roles of the endothelium, KCa channels, perivascular sensory nerves, NO, cannabinoid receptors, and the phospholipase C (PLC)/inositol trisphosphate (InsP3) and RhoA/ROCK signalling pathways, were assessed. OEA caused concentration- and endothelium-dependent vasorelaxation (pEC50=6.7±0.1, Rmax=93.1±2. 5{\%}). L-NAME greatly reduced the response (residual relaxation of only 24.6±12.8{\%}). Capsaicin and pertussis toxin significantly reduced the vasorelaxation. Precontraction with KCl abolished the response. TRAM-34 had no effect, but both iberiotoxin and apamin+charybdotoxin markedly shifted the OEA concentration-response curve to the right (∼5-fold). O-1918 but not rimonabant attenuated the vasorelaxation. Both the CB1 receptor antagonist, AM251 and the CB2 receptor antagonist, AM630, given alone or in combination, reduced the response to OEA. Inhibition of PLC by U73122, ROCK by Y-27632 and antagonism of inositol trisphosphate (InsP3) receptors by 2-APB abolished OEA vasorelaxation. OEA vasorelaxation involves an endothelial site of action but not the known cannabinoid receptors. It involves Ca 2+ released from InsP3-sensitive endothelial stores by mechanisms involving RhoA kinase and phospholipase C. It is likely that the released Ca2+ causes NO generation and opening of mainly large-conductance KCa channels. This study demonstrates a possible novel endothelial target that might be important in the control of regional blood flow induced by this lipid molecule. Crown",
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