Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.)

Hesham A.S. Agrama, Mounir E. Moussa

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Grain yield in the maize (Zea mays L) plant is sensitive to drought in the period three weeks either side of flowering. Maize is well-adapted to the use of restriction fragment length polymorphisms (RFLPs) to identify a tight linkage between gene(s) controlling the quantitative trait and a molecular marker. We have determined the chromosomal locations of quantitative trait loci (QTLs) affecting grain yield under drought, anthesis-silking interval, and number of ears per plant. The F3 families derived from the cross SD34(tolerant) x SD35(intolerant) were evaluated for these traits in a two replicated experiment. RFLP analysis of the maize genome included non- radioactive DNA-DNA hybridization detection using chemiluminescence. To identify QTLs underlying tolerance to drought, the mean phenotypic performances of F3 families were compared based on genotypic classification at each of 70 RFLP marker loci. The genetic linkage map assembled from these markers was in good agreement with previously published maps. The phenotypic correlations between yield and other traits were highly significant. In the combined analyses, genomic regions significantly affecting tolerance to drought were found on chromosomes 1,3,5,6, and 8. For yield, a total of 50% of the phenotypic variance could be explained by five putative QTLs. Different types of gene action were found for the putative QTLs for the three traits.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalEuphytica
Volume91
Issue number1
Publication statusPublished - 1996

Fingerprint

Quantitative Trait Loci
Droughts
drought tolerance
Zea mays
Breeding
quantitative trait loci
drought
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
corn
breeding
grain yield
flowering
Genetic Linkage
Chromosomes, Human, Pair 3
chemiluminescence
Chromosomes, Human, Pair 1
DNA
phenotypic correlation
Luminescence

Keywords

  • anthesis-silking interval
  • drought tolerance
  • grain yield
  • maize
  • QTL
  • RFLP
  • Zea mays

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Cite this

Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.). / Agrama, Hesham A.S.; Moussa, Mounir E.

In: Euphytica, Vol. 91, No. 1, 1996, p. 89-97.

Research output: Contribution to journalArticle

@article{6217a7ad99754e1c838fd88960655dd3,
title = "Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.)",
abstract = "Grain yield in the maize (Zea mays L) plant is sensitive to drought in the period three weeks either side of flowering. Maize is well-adapted to the use of restriction fragment length polymorphisms (RFLPs) to identify a tight linkage between gene(s) controlling the quantitative trait and a molecular marker. We have determined the chromosomal locations of quantitative trait loci (QTLs) affecting grain yield under drought, anthesis-silking interval, and number of ears per plant. The F3 families derived from the cross SD34(tolerant) x SD35(intolerant) were evaluated for these traits in a two replicated experiment. RFLP analysis of the maize genome included non- radioactive DNA-DNA hybridization detection using chemiluminescence. To identify QTLs underlying tolerance to drought, the mean phenotypic performances of F3 families were compared based on genotypic classification at each of 70 RFLP marker loci. The genetic linkage map assembled from these markers was in good agreement with previously published maps. The phenotypic correlations between yield and other traits were highly significant. In the combined analyses, genomic regions significantly affecting tolerance to drought were found on chromosomes 1,3,5,6, and 8. For yield, a total of 50{\%} of the phenotypic variance could be explained by five putative QTLs. Different types of gene action were found for the putative QTLs for the three traits.",
keywords = "anthesis-silking interval, drought tolerance, grain yield, maize, QTL, RFLP, Zea mays",
author = "Agrama, {Hesham A.S.} and Moussa, {Mounir E.}",
year = "1996",
language = "English",
volume = "91",
pages = "89--97",
journal = "Euphytica",
issn = "0014-2336",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - Mapping QTLs in breeding for drought tolerance in maize (Zea mays L.)

AU - Agrama, Hesham A.S.

AU - Moussa, Mounir E.

PY - 1996

Y1 - 1996

N2 - Grain yield in the maize (Zea mays L) plant is sensitive to drought in the period three weeks either side of flowering. Maize is well-adapted to the use of restriction fragment length polymorphisms (RFLPs) to identify a tight linkage between gene(s) controlling the quantitative trait and a molecular marker. We have determined the chromosomal locations of quantitative trait loci (QTLs) affecting grain yield under drought, anthesis-silking interval, and number of ears per plant. The F3 families derived from the cross SD34(tolerant) x SD35(intolerant) were evaluated for these traits in a two replicated experiment. RFLP analysis of the maize genome included non- radioactive DNA-DNA hybridization detection using chemiluminescence. To identify QTLs underlying tolerance to drought, the mean phenotypic performances of F3 families were compared based on genotypic classification at each of 70 RFLP marker loci. The genetic linkage map assembled from these markers was in good agreement with previously published maps. The phenotypic correlations between yield and other traits were highly significant. In the combined analyses, genomic regions significantly affecting tolerance to drought were found on chromosomes 1,3,5,6, and 8. For yield, a total of 50% of the phenotypic variance could be explained by five putative QTLs. Different types of gene action were found for the putative QTLs for the three traits.

AB - Grain yield in the maize (Zea mays L) plant is sensitive to drought in the period three weeks either side of flowering. Maize is well-adapted to the use of restriction fragment length polymorphisms (RFLPs) to identify a tight linkage between gene(s) controlling the quantitative trait and a molecular marker. We have determined the chromosomal locations of quantitative trait loci (QTLs) affecting grain yield under drought, anthesis-silking interval, and number of ears per plant. The F3 families derived from the cross SD34(tolerant) x SD35(intolerant) were evaluated for these traits in a two replicated experiment. RFLP analysis of the maize genome included non- radioactive DNA-DNA hybridization detection using chemiluminescence. To identify QTLs underlying tolerance to drought, the mean phenotypic performances of F3 families were compared based on genotypic classification at each of 70 RFLP marker loci. The genetic linkage map assembled from these markers was in good agreement with previously published maps. The phenotypic correlations between yield and other traits were highly significant. In the combined analyses, genomic regions significantly affecting tolerance to drought were found on chromosomes 1,3,5,6, and 8. For yield, a total of 50% of the phenotypic variance could be explained by five putative QTLs. Different types of gene action were found for the putative QTLs for the three traits.

KW - anthesis-silking interval

KW - drought tolerance

KW - grain yield

KW - maize

KW - QTL

KW - RFLP

KW - Zea mays

UR - http://www.scopus.com/inward/record.url?scp=0029963227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029963227&partnerID=8YFLogxK

M3 - Article

VL - 91

SP - 89

EP - 97

JO - Euphytica

JF - Euphytica

SN - 0014-2336

IS - 1

ER -