Low Usutu virus seroprevalence in four zoological gardens in central Europe

Nicola Buchebner, Wolfgang Zenker, Christian Wenker, Hanspeter W. Steinmetz, Endre Sós, Helga Lussy, Norbert Nowotny

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus antigenic group, caused bird die-offs in Austria, Hungary and Switzerland between 2001 and 2009. While the zoological gardens of Vienna and Zurich recorded USUV-associated mortality in different species of birds during this period, incidences in Budapest were limited to areas outside the zoo, and in the greater Basel area avian mortality due to USUV infection was not observed at all. The objectives of this investigation were to gain insight into USUV infection dynamics in captive birds in zoos with varying degrees of virus exposure and to study differences in susceptibility to USUV of different species of birds.Results: 372 bird sera were collected between October 2006 and August 2007. The samples were tested in parallel by hemagglutination inhibition (HI) and 90% plaque reduction neutralization tests (PRNT-90). 8.75%, 5.3% and 6.59% of birds in the zoos of Vienna, Zurich and Basel, respectively, showed USUV-specific antibodies by PRNT-90. No antibodies to USUV were detected in birds of the Budapest zoo. The order Strigiformes (owls) exhibited the highest USUV-seroprevalence, compared to other orders of birds.Conclusions: USUV seems not to pose an imminent threat to zoo bird populations in central Europe at the moment. Depending on a variety of especially environmental factors, however, this may change at any time in the (near) future, as experienced with West Nile virus (WNV). It is therefore strongly suggested to continue with combined WNV and USUV surveillance activities in affected areas.

Original languageEnglish
Article number153
JournalBMC Veterinary Research
Volume9
DOIs
Publication statusPublished - Aug 6 2013

Fingerprint

Usutu virus
Seroepidemiologic Studies
zoos
Central European region
seroprevalence
Birds
Viruses
birds
Strigiformes
Austria
West Nile virus
Virus Diseases
Austria-Hungary
Japanese Encephalitis Viruses
Flavivirus
Neutralization Tests
Japanese encephalitis virus
Mortality
Antibodies
Hemagglutination

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Low Usutu virus seroprevalence in four zoological gardens in central Europe. / Buchebner, Nicola; Zenker, Wolfgang; Wenker, Christian; Steinmetz, Hanspeter W.; Sós, Endre; Lussy, Helga; Nowotny, Norbert.

In: BMC Veterinary Research, Vol. 9, 153, 06.08.2013.

Research output: Contribution to journalArticle

Buchebner N, Zenker W, Wenker C, Steinmetz HW, Sós E, Lussy H et al. Low Usutu virus seroprevalence in four zoological gardens in central Europe. BMC Veterinary Research. 2013 Aug 6;9. 153. https://doi.org/10.1186/1746-6148-9-153
Buchebner, Nicola ; Zenker, Wolfgang ; Wenker, Christian ; Steinmetz, Hanspeter W. ; Sós, Endre ; Lussy, Helga ; Nowotny, Norbert. / Low Usutu virus seroprevalence in four zoological gardens in central Europe. In: BMC Veterinary Research. 2013 ; Vol. 9.
@article{da086d2e43674db1a946e2e422b0febd,
title = "Low Usutu virus seroprevalence in four zoological gardens in central Europe",
abstract = "Background: Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus antigenic group, caused bird die-offs in Austria, Hungary and Switzerland between 2001 and 2009. While the zoological gardens of Vienna and Zurich recorded USUV-associated mortality in different species of birds during this period, incidences in Budapest were limited to areas outside the zoo, and in the greater Basel area avian mortality due to USUV infection was not observed at all. The objectives of this investigation were to gain insight into USUV infection dynamics in captive birds in zoos with varying degrees of virus exposure and to study differences in susceptibility to USUV of different species of birds.Results: 372 bird sera were collected between October 2006 and August 2007. The samples were tested in parallel by hemagglutination inhibition (HI) and 90{\%} plaque reduction neutralization tests (PRNT-90). 8.75{\%}, 5.3{\%} and 6.59{\%} of birds in the zoos of Vienna, Zurich and Basel, respectively, showed USUV-specific antibodies by PRNT-90. No antibodies to USUV were detected in birds of the Budapest zoo. The order Strigiformes (owls) exhibited the highest USUV-seroprevalence, compared to other orders of birds.Conclusions: USUV seems not to pose an imminent threat to zoo bird populations in central Europe at the moment. Depending on a variety of especially environmental factors, however, this may change at any time in the (near) future, as experienced with West Nile virus (WNV). It is therefore strongly suggested to continue with combined WNV and USUV surveillance activities in affected areas.",
author = "Nicola Buchebner and Wolfgang Zenker and Christian Wenker and Steinmetz, {Hanspeter W.} and Endre S{\'o}s and Helga Lussy and Norbert Nowotny",
year = "2013",
month = "8",
day = "6",
doi = "10.1186/1746-6148-9-153",
language = "English",
volume = "9",
journal = "BMC Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Low Usutu virus seroprevalence in four zoological gardens in central Europe

AU - Buchebner, Nicola

AU - Zenker, Wolfgang

AU - Wenker, Christian

AU - Steinmetz, Hanspeter W.

AU - Sós, Endre

AU - Lussy, Helga

AU - Nowotny, Norbert

PY - 2013/8/6

Y1 - 2013/8/6

N2 - Background: Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus antigenic group, caused bird die-offs in Austria, Hungary and Switzerland between 2001 and 2009. While the zoological gardens of Vienna and Zurich recorded USUV-associated mortality in different species of birds during this period, incidences in Budapest were limited to areas outside the zoo, and in the greater Basel area avian mortality due to USUV infection was not observed at all. The objectives of this investigation were to gain insight into USUV infection dynamics in captive birds in zoos with varying degrees of virus exposure and to study differences in susceptibility to USUV of different species of birds.Results: 372 bird sera were collected between October 2006 and August 2007. The samples were tested in parallel by hemagglutination inhibition (HI) and 90% plaque reduction neutralization tests (PRNT-90). 8.75%, 5.3% and 6.59% of birds in the zoos of Vienna, Zurich and Basel, respectively, showed USUV-specific antibodies by PRNT-90. No antibodies to USUV were detected in birds of the Budapest zoo. The order Strigiformes (owls) exhibited the highest USUV-seroprevalence, compared to other orders of birds.Conclusions: USUV seems not to pose an imminent threat to zoo bird populations in central Europe at the moment. Depending on a variety of especially environmental factors, however, this may change at any time in the (near) future, as experienced with West Nile virus (WNV). It is therefore strongly suggested to continue with combined WNV and USUV surveillance activities in affected areas.

AB - Background: Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus antigenic group, caused bird die-offs in Austria, Hungary and Switzerland between 2001 and 2009. While the zoological gardens of Vienna and Zurich recorded USUV-associated mortality in different species of birds during this period, incidences in Budapest were limited to areas outside the zoo, and in the greater Basel area avian mortality due to USUV infection was not observed at all. The objectives of this investigation were to gain insight into USUV infection dynamics in captive birds in zoos with varying degrees of virus exposure and to study differences in susceptibility to USUV of different species of birds.Results: 372 bird sera were collected between October 2006 and August 2007. The samples were tested in parallel by hemagglutination inhibition (HI) and 90% plaque reduction neutralization tests (PRNT-90). 8.75%, 5.3% and 6.59% of birds in the zoos of Vienna, Zurich and Basel, respectively, showed USUV-specific antibodies by PRNT-90. No antibodies to USUV were detected in birds of the Budapest zoo. The order Strigiformes (owls) exhibited the highest USUV-seroprevalence, compared to other orders of birds.Conclusions: USUV seems not to pose an imminent threat to zoo bird populations in central Europe at the moment. Depending on a variety of especially environmental factors, however, this may change at any time in the (near) future, as experienced with West Nile virus (WNV). It is therefore strongly suggested to continue with combined WNV and USUV surveillance activities in affected areas.

UR - http://www.scopus.com/inward/record.url?scp=84881003587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881003587&partnerID=8YFLogxK

U2 - 10.1186/1746-6148-9-153

DO - 10.1186/1746-6148-9-153

M3 - Article

VL - 9

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

M1 - 153

ER -