Long-term compositional changes after transplant in a microbial mat cyanobacterial community revealed using a polyphasic approach

Raeid M M Abed, Ferran Garcia-Pichel

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Using a polyphasic approach that included microscopy, cultivation and 16S rRNA-based cultivation-independent molecular fingerprinting, we compared the cyanobacterial composition of Solar Lake microbial mats and samples thereof transplanted and maintained in new settings for extended periods of time. Significant changes in community composition, with clear replacement of the dominant cyanobacterium, Microcoleus chthonoplastes, were detected in all cases. The most dramatic shifts occurred in a sample kept in the laboratory for 3 years, which resulted in dominance by an Oscillatoria-like cyanobacterium whose 16S rRNA closely matched that of a morphologically similar isolate from mats in Mexico. Transfer of Solar Lake mat to an artificial experimental pond with incubation under seminatural conditions resulted in an increase in cyanobacterial diversity. Judging from the molecular signatures, two novel, previously unrecognized and phylogenetically well-delimited cyanobacterial populations became dominant. Through cultivation, one population was shown to correspond to a filamentous, non-heterocystous group of Cyanobacteria with very narrow trichomes (≅0.75-1.5 μm). The most dominant novel molecular signature, however, could not be identified by cultivation efforts or correlation with microscopy and, upon phylogenetic analyses, its 16S rRNA genes showed no particular close association to known cyanobacterial groups.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalEnvironmental Microbiology
Volume3
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

microbial mat
Cyanobacteria
long-term change
ribosomal RNA
Lakes
Transplants
cyanobacterium
Microscopy
microscopy
Microcoleus chthonoplastes
Oscillatoria
Trichomes
lakes
Mexico
rRNA Genes
trichomes
Population
trichome
lake
community composition

ASJC Scopus subject areas

  • Environmental Science(all)
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Long-term compositional changes after transplant in a microbial mat cyanobacterial community revealed using a polyphasic approach. / Abed, Raeid M M; Garcia-Pichel, Ferran.

In: Environmental Microbiology, Vol. 3, No. 1, 2001, p. 53-62.

Research output: Contribution to journalArticle

@article{b463f96da1f649dabeee0a734eef811a,
title = "Long-term compositional changes after transplant in a microbial mat cyanobacterial community revealed using a polyphasic approach",
abstract = "Using a polyphasic approach that included microscopy, cultivation and 16S rRNA-based cultivation-independent molecular fingerprinting, we compared the cyanobacterial composition of Solar Lake microbial mats and samples thereof transplanted and maintained in new settings for extended periods of time. Significant changes in community composition, with clear replacement of the dominant cyanobacterium, Microcoleus chthonoplastes, were detected in all cases. The most dramatic shifts occurred in a sample kept in the laboratory for 3 years, which resulted in dominance by an Oscillatoria-like cyanobacterium whose 16S rRNA closely matched that of a morphologically similar isolate from mats in Mexico. Transfer of Solar Lake mat to an artificial experimental pond with incubation under seminatural conditions resulted in an increase in cyanobacterial diversity. Judging from the molecular signatures, two novel, previously unrecognized and phylogenetically well-delimited cyanobacterial populations became dominant. Through cultivation, one population was shown to correspond to a filamentous, non-heterocystous group of Cyanobacteria with very narrow trichomes (≅0.75-1.5 μm). The most dominant novel molecular signature, however, could not be identified by cultivation efforts or correlation with microscopy and, upon phylogenetic analyses, its 16S rRNA genes showed no particular close association to known cyanobacterial groups.",
author = "Abed, {Raeid M M} and Ferran Garcia-Pichel",
year = "2001",
doi = "10.1046/j.1462-2920.2001.00159.x",
language = "English",
volume = "3",
pages = "53--62",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Long-term compositional changes after transplant in a microbial mat cyanobacterial community revealed using a polyphasic approach

AU - Abed, Raeid M M

AU - Garcia-Pichel, Ferran

PY - 2001

Y1 - 2001

N2 - Using a polyphasic approach that included microscopy, cultivation and 16S rRNA-based cultivation-independent molecular fingerprinting, we compared the cyanobacterial composition of Solar Lake microbial mats and samples thereof transplanted and maintained in new settings for extended periods of time. Significant changes in community composition, with clear replacement of the dominant cyanobacterium, Microcoleus chthonoplastes, were detected in all cases. The most dramatic shifts occurred in a sample kept in the laboratory for 3 years, which resulted in dominance by an Oscillatoria-like cyanobacterium whose 16S rRNA closely matched that of a morphologically similar isolate from mats in Mexico. Transfer of Solar Lake mat to an artificial experimental pond with incubation under seminatural conditions resulted in an increase in cyanobacterial diversity. Judging from the molecular signatures, two novel, previously unrecognized and phylogenetically well-delimited cyanobacterial populations became dominant. Through cultivation, one population was shown to correspond to a filamentous, non-heterocystous group of Cyanobacteria with very narrow trichomes (≅0.75-1.5 μm). The most dominant novel molecular signature, however, could not be identified by cultivation efforts or correlation with microscopy and, upon phylogenetic analyses, its 16S rRNA genes showed no particular close association to known cyanobacterial groups.

AB - Using a polyphasic approach that included microscopy, cultivation and 16S rRNA-based cultivation-independent molecular fingerprinting, we compared the cyanobacterial composition of Solar Lake microbial mats and samples thereof transplanted and maintained in new settings for extended periods of time. Significant changes in community composition, with clear replacement of the dominant cyanobacterium, Microcoleus chthonoplastes, were detected in all cases. The most dramatic shifts occurred in a sample kept in the laboratory for 3 years, which resulted in dominance by an Oscillatoria-like cyanobacterium whose 16S rRNA closely matched that of a morphologically similar isolate from mats in Mexico. Transfer of Solar Lake mat to an artificial experimental pond with incubation under seminatural conditions resulted in an increase in cyanobacterial diversity. Judging from the molecular signatures, two novel, previously unrecognized and phylogenetically well-delimited cyanobacterial populations became dominant. Through cultivation, one population was shown to correspond to a filamentous, non-heterocystous group of Cyanobacteria with very narrow trichomes (≅0.75-1.5 μm). The most dominant novel molecular signature, however, could not be identified by cultivation efforts or correlation with microscopy and, upon phylogenetic analyses, its 16S rRNA genes showed no particular close association to known cyanobacterial groups.

UR - http://www.scopus.com/inward/record.url?scp=0035194375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035194375&partnerID=8YFLogxK

U2 - 10.1046/j.1462-2920.2001.00159.x

DO - 10.1046/j.1462-2920.2001.00159.x

M3 - Article

C2 - 11225723

AN - SCOPUS:0035194375

VL - 3

SP - 53

EP - 62

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 1

ER -