Local differentiation in Plasmodium falciparum drug resistance genes in Sudan

A. A. Abdel-Muhsin, M. J. Mackinnon, P. Awadalla, E. Ali, S. Suleiman, S. Ahmed, D. Walliker, H. A. Babiker

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Abstract

Studies of population genetic structure of parasites can be used to infer which parasite genes are under selection. Here, the population structure of 4 genes associated with drug resistance of Plasmodium falciparum (the chloroquine resistance transporter, pfcrt, dihydrofolate reductase, dhfr, dihydropteroate synthase, dhps, and multi-drug resistance, pfmdr-1) were examined in parasite populations in 3 villages in eastern Sudan and in an urban area of Khartoum, the capital. In order to differentiate the effects of drug selection from neutral influences on population structure, parasites were also genotyped for 3 putatively neutral microsatellite loci (polya, TA81 and pfg377), and for 2 antigenic loci that are either under balancing selection or neutral, merozoite surface protein 1 and 2, (MSP-1 and MSP-2). Cross-sectional surveys were carried out during the peak transmission (wet) season and in the ensuing dry season. No significant variation in frequencies of MSP-1 and MSP-2 alleles was seen among villages in the eastern region and between the villages and Khartoum, nor between the wet and dry season. However, the drug resistance genes, pfmdr-1, pfcrt and dhfr and to a lesser extent the microsatellite loci showed high FST values when comparing villages with Khartoum, indicating strong geographical differentiation at these loci. Moreover, variation in frequencies of the drug resistance genes, pfmdr-1, pfcrt and dhfr, was observed between the wet and dry season. These differences most probably reflect the variation in drug pressure between each region, and in drug usage between the wet and dry season in a given region.

Original languageEnglish
Pages (from-to)391-400
Number of pages10
JournalParasitology
Volume126
Issue number5
DOIs
Publication statusPublished - May 1 2003

Fingerprint

Sudan
drug resistance
Plasmodium falciparum
Drug Resistance
Merozoite Surface Protein 1
villages
dry season
wet season
Parasites
parasites
loci
drugs
Genes
population structure
genes
Microsatellite Repeats
microsatellite repeats
dihydrofolate reductase
chloroquine
Dihydropteroate Synthase

Keywords

  • Antimalarials
  • Drug resistance genes
  • Malaria
  • Plasmodium falciparum
  • Selection

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Abdel-Muhsin, A. A., Mackinnon, M. J., Awadalla, P., Ali, E., Suleiman, S., Ahmed, S., ... Babiker, H. A. (2003). Local differentiation in Plasmodium falciparum drug resistance genes in Sudan. Parasitology, 126(5), 391-400. https://doi.org/10.1017/S0031182003003020

Local differentiation in Plasmodium falciparum drug resistance genes in Sudan. / Abdel-Muhsin, A. A.; Mackinnon, M. J.; Awadalla, P.; Ali, E.; Suleiman, S.; Ahmed, S.; Walliker, D.; Babiker, H. A.

In: Parasitology, Vol. 126, No. 5, 01.05.2003, p. 391-400.

Research output: Contribution to journalArticle

Abdel-Muhsin, AA, Mackinnon, MJ, Awadalla, P, Ali, E, Suleiman, S, Ahmed, S, Walliker, D & Babiker, HA 2003, 'Local differentiation in Plasmodium falciparum drug resistance genes in Sudan', Parasitology, vol. 126, no. 5, pp. 391-400. https://doi.org/10.1017/S0031182003003020
Abdel-Muhsin AA, Mackinnon MJ, Awadalla P, Ali E, Suleiman S, Ahmed S et al. Local differentiation in Plasmodium falciparum drug resistance genes in Sudan. Parasitology. 2003 May 1;126(5):391-400. https://doi.org/10.1017/S0031182003003020
Abdel-Muhsin, A. A. ; Mackinnon, M. J. ; Awadalla, P. ; Ali, E. ; Suleiman, S. ; Ahmed, S. ; Walliker, D. ; Babiker, H. A. / Local differentiation in Plasmodium falciparum drug resistance genes in Sudan. In: Parasitology. 2003 ; Vol. 126, No. 5. pp. 391-400.
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