Liquid chromatography-tandem mass spectroscopic method for the determination of zerumbone in human plasma and its application to pharmacokinetics.

Eltayeb E M Eid, Ahmad Bustamam Abdul, A. Rasedee, Fakhr Eldin O Suliman, Mohd A. Sukari, Safa A. Fatah

Research output: Contribution to journalArticle

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Abstract

A rapid, sensitive, specific and selective LC-MS/MS method for the determination of zerumbone (ZER) in human plasma using 2,4-diamino-6-(4-methoxyphenyl)-1,3,5-triazine (DMTZ) as an internal standard (IS) has been developed and validated. ZER was chromatographed on C8 column using a mobile phase of acetonitrile/water (80:20, v/v) at a flow rate of 0.25 ml min(-1) . Quantitation was achieved using ESI+ interface, employing multiple reaction monitoring (MRM) mode at m/z 219 > 81 and 218 > 134 for ZER and IS, respectively. The calibration standards were linear over a range of 5-3000 ng ml(-1) (r(2)=0.9994) with an LLOQ of 5 ng ml(-1) (RSD %; 11.4% and bias%; 9.5%). Intra- and inter-day precision of ZER assay ranged from 0.18 to 3.56% with accuracy (bias) that varied between -5.09 and 4.3%, demonstrating good precision and accuracy. Recoveries of ZER and the IS from human plasma were above 85%. The developed method was validated for the determination of ZER in rat plasma. Linearity, stability of ZER and the ME on rat plasma were discussed. The applicability of the developed method was demonstrated by measuring ZER in rat plasma samples following intravenous and intraperitoneal administration of ZER prepared in hydroxypropyl-β-cyclodextrin (HPβCD) and sodium carboxymethyl cellulose (CMC), respectively, in 20 mg kg(-1) and this study indicated a clear significant difference (p

Original languageEnglish
Pages (from-to)772-781
Number of pages10
JournalJournal of mass spectrometry : JMS
Volume46
Issue number8
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

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Plasma (human)
Pharmacokinetics
Liquid chromatography
Liquid Chromatography
Rats
Plasmas
zerumbone
Triazines
Carboxymethylcellulose Sodium
Cyclodextrins
Intravenous Administration
Calibration
Assays
Sodium
Flow rate
Recovery

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Liquid chromatography-tandem mass spectroscopic method for the determination of zerumbone in human plasma and its application to pharmacokinetics. / Eid, Eltayeb E M; Abdul, Ahmad Bustamam; Rasedee, A.; Suliman, Fakhr Eldin O; Sukari, Mohd A.; Fatah, Safa A.

In: Journal of mass spectrometry : JMS, Vol. 46, No. 8, 08.2011, p. 772-781.

Research output: Contribution to journalArticle

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abstract = "A rapid, sensitive, specific and selective LC-MS/MS method for the determination of zerumbone (ZER) in human plasma using 2,4-diamino-6-(4-methoxyphenyl)-1,3,5-triazine (DMTZ) as an internal standard (IS) has been developed and validated. ZER was chromatographed on C8 column using a mobile phase of acetonitrile/water (80:20, v/v) at a flow rate of 0.25 ml min(-1) . Quantitation was achieved using ESI+ interface, employing multiple reaction monitoring (MRM) mode at m/z 219 > 81 and 218 > 134 for ZER and IS, respectively. The calibration standards were linear over a range of 5-3000 ng ml(-1) (r(2)=0.9994) with an LLOQ of 5 ng ml(-1) (RSD {\%}; 11.4{\%} and bias{\%}; 9.5{\%}). Intra- and inter-day precision of ZER assay ranged from 0.18 to 3.56{\%} with accuracy (bias) that varied between -5.09 and 4.3{\%}, demonstrating good precision and accuracy. Recoveries of ZER and the IS from human plasma were above 85{\%}. The developed method was validated for the determination of ZER in rat plasma. Linearity, stability of ZER and the ME on rat plasma were discussed. The applicability of the developed method was demonstrated by measuring ZER in rat plasma samples following intravenous and intraperitoneal administration of ZER prepared in hydroxypropyl-β-cyclodextrin (HPβCD) and sodium carboxymethyl cellulose (CMC), respectively, in 20 mg kg(-1) and this study indicated a clear significant difference (p",
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