Lipopeptide production by Bacillus subtilis R1 and its possible applications

Sujata S. Jha, Sanket J. Joshi, S. J. Geetha

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The possible application of a bacterial strain – Bacillus subtilis R1, isolated from an oil contaminated desert site in India, as biocontrol agent and its biosurfactant in microbial enhanced oil recovery are discussed. The biosurfactant production in minimal medium was carried out at different temperatures and salt concentrations, where it produced an efficient biosurfactant at 30–45 °C and in presence of up to 7% salt. It significantly reduced the surface tension from 66 ± 1.25 mN/m to 29 ± 0.85 mN/m within 24 h. In order to enhance the biosurfactant production, random mutagenesis of B. subtilis R1 was performed using chemical mutagen – ethyl methanesulfonate. Majority of the isolated 42 mutants showed biosurfactant production, but the difference was statistically insignificant as compared with parent strain R1. Therefore none of the mutants were selected for further study, and only parent strain R1 was studied. The biosurfactant was quite stable under harsh conditions for up to 10 days. The biosurfactant was extracted and characterized as similar to the lipopeptide group – surfactins and fengycin. The crude oil displacement experiments using biosurfactant broth in sand pack glass columns showed 33 ± 1.25% additional oil recovery. The strain also showed inhibition of various plant pathogenic fungi on potato dextrose agar medium.

Original languageEnglish
Pages (from-to)955-964
Number of pages10
JournalBrazilian Journal of Microbiology
Volume47
Issue number4
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Lipopeptides
Bacillus subtilis
Oils
Salts
Ethyl Methanesulfonate
Surface Tension
Petroleum
Mutagens
Solanum tuberosum
Mutagenesis
Agar
Glass
India
Fungi
Glucose
Temperature

Keywords

  • Antifungal activity
  • Bacillus subtilis
  • Biosurfactant
  • Ethyl methanesulfonate
  • Microbial enhanced oil recovery

ASJC Scopus subject areas

  • Microbiology

Cite this

Lipopeptide production by Bacillus subtilis R1 and its possible applications. / Jha, Sujata S.; Joshi, Sanket J.; Geetha, S. J.

In: Brazilian Journal of Microbiology, Vol. 47, No. 4, 01.10.2016, p. 955-964.

Research output: Contribution to journalArticle

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