Limited pathogenicity of usutu virus for the domestic goose (Anser anser f. domestica) following experimental inoculation

S. Chvala, T. Bakonyi, R. Hackl, M. Hess, N. Nowotny, H. Weissenböck

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Usutu virus (USUV) is a mosquito-borne flavivirus of the Japanese encephalitis virus group, which has been associated with avian mortality in Austria since 2001. The affected birds are predominantly Eurasian blackbirds (Turdus merula). In the present study, the pathogenicity of USUV for domestic geese (Anser anser f. domestica) was investigated. Eleven 2-week-old geese were inoculated intramuscularly with 5 × 104 50% tissue culture infectious dose of USUV strain Vienna-2001 blackbird. No clinical signs were seen during the observation period. Four inoculated and one in-contact geese died without preceding clinical signs. Two of the deaths could be attributed to bacterial septicaemia and strangulation, respectively. The cause of death of two experimental and one in-contact animals remained unclear, but lack of evidence for viral lesions and viral antigen in their tissues argued against association with the USUV infection. Although in organs of the majority of inoculated geese (9/11) USUV was detected by reverse transcriptase-polymerase chain reaction, immunohistochemistry for USUV antigen was negative in all tissues of all geese. Evidence of plasma viraemia or viral excretion was found in one goose only. Seroconversion was detected in three inoculated geese 10 days post-inoculation. Geese placed in contact with inoculated geese and control animals did not exhibit USUV in their internal organs or plasma and lacked USUV-specific antibodies. This experiment shows that USUV is able to replicate in geese, but does not induce clinical disease, is unlikely to induce mortality, and only infrequently leads to viraemia or virus shedding.

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalJournal of Veterinary Medicine, Series B
Volume53
Issue number4
DOIs
Publication statusPublished - May 2006

Fingerprint

Usutu virus
Anser anser
Geese
geese
Virulence
pathogenicity
Viruses
Viremia
viremia
Austria
death
Japanese Encephalitis Viruses
Japanese encephalitis virus
Virus Shedding
Turdus merula
Flavivirus
viral shedding
Mortality
Viral Antigens
viral antigens

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)
  • Microbiology

Cite this

Limited pathogenicity of usutu virus for the domestic goose (Anser anser f. domestica) following experimental inoculation. / Chvala, S.; Bakonyi, T.; Hackl, R.; Hess, M.; Nowotny, N.; Weissenböck, H.

In: Journal of Veterinary Medicine, Series B, Vol. 53, No. 4, 05.2006, p. 171-175.

Research output: Contribution to journalArticle

@article{a6db1c5566cf4b4da9cfb8bde4da862c,
title = "Limited pathogenicity of usutu virus for the domestic goose (Anser anser f. domestica) following experimental inoculation",
abstract = "Usutu virus (USUV) is a mosquito-borne flavivirus of the Japanese encephalitis virus group, which has been associated with avian mortality in Austria since 2001. The affected birds are predominantly Eurasian blackbirds (Turdus merula). In the present study, the pathogenicity of USUV for domestic geese (Anser anser f. domestica) was investigated. Eleven 2-week-old geese were inoculated intramuscularly with 5 × 104 50{\%} tissue culture infectious dose of USUV strain Vienna-2001 blackbird. No clinical signs were seen during the observation period. Four inoculated and one in-contact geese died without preceding clinical signs. Two of the deaths could be attributed to bacterial septicaemia and strangulation, respectively. The cause of death of two experimental and one in-contact animals remained unclear, but lack of evidence for viral lesions and viral antigen in their tissues argued against association with the USUV infection. Although in organs of the majority of inoculated geese (9/11) USUV was detected by reverse transcriptase-polymerase chain reaction, immunohistochemistry for USUV antigen was negative in all tissues of all geese. Evidence of plasma viraemia or viral excretion was found in one goose only. Seroconversion was detected in three inoculated geese 10 days post-inoculation. Geese placed in contact with inoculated geese and control animals did not exhibit USUV in their internal organs or plasma and lacked USUV-specific antibodies. This experiment shows that USUV is able to replicate in geese, but does not induce clinical disease, is unlikely to induce mortality, and only infrequently leads to viraemia or virus shedding.",
author = "S. Chvala and T. Bakonyi and R. Hackl and M. Hess and N. Nowotny and H. Weissenb{\"o}ck",
year = "2006",
month = "5",
doi = "10.1111/j.1439-0450.2006.00942.x",
language = "English",
volume = "53",
pages = "171--175",
journal = "Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health",
issn = "1863-1959",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Limited pathogenicity of usutu virus for the domestic goose (Anser anser f. domestica) following experimental inoculation

AU - Chvala, S.

AU - Bakonyi, T.

AU - Hackl, R.

AU - Hess, M.

AU - Nowotny, N.

AU - Weissenböck, H.

PY - 2006/5

Y1 - 2006/5

N2 - Usutu virus (USUV) is a mosquito-borne flavivirus of the Japanese encephalitis virus group, which has been associated with avian mortality in Austria since 2001. The affected birds are predominantly Eurasian blackbirds (Turdus merula). In the present study, the pathogenicity of USUV for domestic geese (Anser anser f. domestica) was investigated. Eleven 2-week-old geese were inoculated intramuscularly with 5 × 104 50% tissue culture infectious dose of USUV strain Vienna-2001 blackbird. No clinical signs were seen during the observation period. Four inoculated and one in-contact geese died without preceding clinical signs. Two of the deaths could be attributed to bacterial septicaemia and strangulation, respectively. The cause of death of two experimental and one in-contact animals remained unclear, but lack of evidence for viral lesions and viral antigen in their tissues argued against association with the USUV infection. Although in organs of the majority of inoculated geese (9/11) USUV was detected by reverse transcriptase-polymerase chain reaction, immunohistochemistry for USUV antigen was negative in all tissues of all geese. Evidence of plasma viraemia or viral excretion was found in one goose only. Seroconversion was detected in three inoculated geese 10 days post-inoculation. Geese placed in contact with inoculated geese and control animals did not exhibit USUV in their internal organs or plasma and lacked USUV-specific antibodies. This experiment shows that USUV is able to replicate in geese, but does not induce clinical disease, is unlikely to induce mortality, and only infrequently leads to viraemia or virus shedding.

AB - Usutu virus (USUV) is a mosquito-borne flavivirus of the Japanese encephalitis virus group, which has been associated with avian mortality in Austria since 2001. The affected birds are predominantly Eurasian blackbirds (Turdus merula). In the present study, the pathogenicity of USUV for domestic geese (Anser anser f. domestica) was investigated. Eleven 2-week-old geese were inoculated intramuscularly with 5 × 104 50% tissue culture infectious dose of USUV strain Vienna-2001 blackbird. No clinical signs were seen during the observation period. Four inoculated and one in-contact geese died without preceding clinical signs. Two of the deaths could be attributed to bacterial septicaemia and strangulation, respectively. The cause of death of two experimental and one in-contact animals remained unclear, but lack of evidence for viral lesions and viral antigen in their tissues argued against association with the USUV infection. Although in organs of the majority of inoculated geese (9/11) USUV was detected by reverse transcriptase-polymerase chain reaction, immunohistochemistry for USUV antigen was negative in all tissues of all geese. Evidence of plasma viraemia or viral excretion was found in one goose only. Seroconversion was detected in three inoculated geese 10 days post-inoculation. Geese placed in contact with inoculated geese and control animals did not exhibit USUV in their internal organs or plasma and lacked USUV-specific antibodies. This experiment shows that USUV is able to replicate in geese, but does not induce clinical disease, is unlikely to induce mortality, and only infrequently leads to viraemia or virus shedding.

UR - http://www.scopus.com/inward/record.url?scp=33646468681&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646468681&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0450.2006.00942.x

DO - 10.1111/j.1439-0450.2006.00942.x

M3 - Article

C2 - 16629984

AN - SCOPUS:33646468681

VL - 53

SP - 171

EP - 175

JO - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

JF - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

SN - 1863-1959

IS - 4

ER -