Abstract
Cytoplasmic methionyl tRNA synthetase (MetRS) is one of more than 20 cytoplasmic aminoacyl tRNA synthetase enzymes (ARS). This family of enzymes catalyzes a process fundamental for protein translation. Using a combination of genetic mapping, oligonucleotide array comparative genomic hybridization, and phenotypic correlation, we show that mutations in the essential gene, let-65, reside within the predicted Caenorhabditis elegans homologue of MetRS, which we have named mars-1. We demonstrate that the lethality associated with alleles of let-65 is fully rescued by a transgenic array that spans the mars-1 genomic region. Furthermore, sequence analysis reveals that six let-65 alleles lead to the alteration of highly conserved amino acids.
Original language | Undefined/Unknown |
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Pages (from-to) | 819-830 |
Number of pages | 12 |
Journal | Meta Gene |
Volume | 2 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- Aminoacyl tRNA synthetase, methionyl-tRNA synthetase
- Caenorhabditis elegans
- Essential genes
- Let-65
- Oligonucleotide array comparative genomic hybridization
ASJC Scopus subject areas
- Genetics(clinical)
- Genetics