Kinetics of drug release from a hyaluronan-steroid conjugate investigated by NMR spectroscopy

Anna Taglienti, Paolo Sequi, Massimiliano Valentini

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The methylprednisolone steroid ester of hyaluronan was hydrolyzed under physiological conditions in vitro, and the kinetics of drug release was investigated by NMR spectroscopy. Transverse relaxation times are correlated with the molecular rotational freedom, which undergoes large changes for methylprednisolone when released. Multi-exponential decays were observed, which together with the corresponding population gave valuable insights into the conformational changes that occur in the biopolymer during hydrolysis. The biomaterial exists in aqueous solution in two conformations, 'collapsed' and 'water-exposed', in equilibrium. Under physiological conditions, the methylprednisolone is completely released within 48 h. Transverse relaxation times proved to be an appropriate tool for monitoring the drug release in vitro.

Original languageEnglish
Pages (from-to)245-249
Number of pages5
JournalCarbohydrate Research
Volume344
Issue number2
DOIs
Publication statusPublished - Jan 26 2009

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Keywords

  • Hyaluronan
  • Methylprednisolone
  • NMR spectroscopy
  • Release
  • Transverse relaxation times

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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