Abstract
The methylprednisolone steroid ester of hyaluronan was hydrolyzed under physiological conditions in vitro, and the kinetics of drug release was investigated by NMR spectroscopy. Transverse relaxation times are correlated with the molecular rotational freedom, which undergoes large changes for methylprednisolone when released. Multi-exponential decays were observed, which together with the corresponding population gave valuable insights into the conformational changes that occur in the biopolymer during hydrolysis. The biomaterial exists in aqueous solution in two conformations, 'collapsed' and 'water-exposed', in equilibrium. Under physiological conditions, the methylprednisolone is completely released within 48 h. Transverse relaxation times proved to be an appropriate tool for monitoring the drug release in vitro.
Original language | English |
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Pages (from-to) | 245-249 |
Number of pages | 5 |
Journal | Carbohydrate Research |
Volume | 344 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jan 26 2009 |
Externally published | Yes |
Keywords
- Hyaluronan
- Methylprednisolone
- NMR spectroscopy
- Release
- Transverse relaxation times
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry