Key role of mucosal primary afferents in mediating the inhibitory influence of capsaicin on vagally mediated contractions in the mouse esophagus

Ammar Boudaka, Jürgen Wörl, Takahiko Shiina, Shouichiro Saito, Yasuro Atoji, Haruo Kobayashi, Yasutake Shimizu, Tadashi Takewaki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Transient receptor potential ion channel of the vanilloid type 1 (TRPV1)-dependent pathway, consisting of capsaicin-sensitive tachykininergic primary afferent and myenteric nitrergic neurons, was suggested to mediate the inhibitory effect of capsaicin on the vagally mediated striated muscle contractions in the rat esophagus. These primary afferent neurons upon entering into the esophagus are distributed through the myenteric plexus, terminating either in the myenteric ganglia or en route to the mucosa where they branch into a delicate net of fine varicose fibers. Therefore, this study aimed to investigate whether the mucosal primary afferents are a main mediator for the capsaicin inhibitory influence on vagally mediated contractions in the mouse esophagus. For this purpose, the vagally induced contractile activity of a thoracic esophageal segment was measured in the circular direction with a force transducer. Vagal stimulation (30 μsec, 25 V, 1-50 Hz for 1 sec) produced monophasic contractile responses, whose amplitudes were frequency-dependent. These contractions were completely abolished by d-tubocurarine (5 μM) while resistant to atropine (1 μM) and hexamethonium (100 μM). Capsaicin (30 μM) significantly inhibited the vagally induced contractions in esophagi with intact mucosa while its effect on preparations without mucosa was insignificant. Additionally, immunocytochemistry revealed the presence of TRPV1-positive nerve fibers in the tunica mucosa. Taken together, we conclude that in the mouse esophagus, capsaicin inhibits the vagally mediated striated muscle contractions mainly through its action on mucosal primary afferents, which in turn activate the presumed inhibitory local reflex arc.

Original languageEnglish
Pages (from-to)365-372
Number of pages8
JournalJournal of Veterinary Medical Science
Volume69
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

capsaicin
Capsaicin
esophagus
Esophagus
mucosa
Mucous Membrane
mice
striated muscle
Striated Muscle
muscle contraction
Muscle Contraction
Nitrergic Neurons
Transient Receptor Potential Channels
Afferent Neurons
Tubocurarine
Myenteric Plexus
Hexamethonium
sensory neurons
atropine
plexus

Keywords

  • Capsaicin-sensitive neurons
  • Enteric co-innervation
  • Local effector function
  • TRPV1
  • Vagus nerve

ASJC Scopus subject areas

  • veterinary(all)
  • Marketing

Cite this

Key role of mucosal primary afferents in mediating the inhibitory influence of capsaicin on vagally mediated contractions in the mouse esophagus. / Boudaka, Ammar; Wörl, Jürgen; Shiina, Takahiko; Saito, Shouichiro; Atoji, Yasuro; Kobayashi, Haruo; Shimizu, Yasutake; Takewaki, Tadashi.

In: Journal of Veterinary Medical Science, Vol. 69, No. 4, 04.2007, p. 365-372.

Research output: Contribution to journalArticle

Boudaka, Ammar ; Wörl, Jürgen ; Shiina, Takahiko ; Saito, Shouichiro ; Atoji, Yasuro ; Kobayashi, Haruo ; Shimizu, Yasutake ; Takewaki, Tadashi. / Key role of mucosal primary afferents in mediating the inhibitory influence of capsaicin on vagally mediated contractions in the mouse esophagus. In: Journal of Veterinary Medical Science. 2007 ; Vol. 69, No. 4. pp. 365-372.
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