Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis

Rengasamy Balakrishnan, Namasivayam Elangovan, Thangavel Mohankumar, Jegadeesan Nataraj, Thamilarasan Manivasagam, Arokiasamy Justin Thenmozhi, Musthafa Mohamed Essa, Mohammed Akbar, Mohammed Abdul Sattar Khan

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Abstract

The present study was carried out to investigate the neuroprotective effects of isolongifolene (ILF), a tricyclic sesquiterpene of Murraya koenigii, against rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis in a cellular model. SH-SY5Y human neuroblastoma cells were divided into four experimental groups (control, rotenone (100 nM), ILF (10 microM) + rotenone (100 nanoM), ILF 10 microM alone treated) based on 3-(4, 5-dimethyl 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results of the present study showed that the ILF treatment significantly alleviated rotenone-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in SH-SY5Y cells. Moreover, ILF attenuated rotenone induced toxicity by down-regulating  Bax, caspases-3, 6, 8 and 9 expression and up-regulating of Bcl-2 expression. Furthermore regulation of p-P13K, p-AKT and p-GSK-3 beta expression by ILF, clearly confirmed its protective effects. Taken together, our results suggested that ILF attenuated rotenone-induced oxidative stress, mitochondrial dysfunction and apoptosis through the regulation of P13K/AKT/GSK-3 beta signaling pathways. However further pre-clinical studies are warranted in rodents to use ILF as a promising therapeutic agent for PD in future.

Original languageEnglish
Pages (from-to)248-261
Number of pages14
JournalFrontiers in bioscience (Scholar edition)
Volume10
Publication statusPublished - Jan 1 2018

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Rotenone
Oxidative stress
Oxidative Stress
Apoptosis
Glycogen Synthase Kinase 3
Murraya
Caspase 6
Caspase 9
Sesquiterpenes
Neuroprotective Agents
Cytotoxicity
Bromides
Neuroblastoma
Caspase 3
Toxicity
Rodentia
Assays
Control Groups

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Balakrishnan, R., Elangovan, N., Mohankumar, T., Nataraj, J., Manivasagam, T., Justin Thenmozhi, A., ... Abdul Sattar Khan, M. (2018). Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis. Frontiers in bioscience (Scholar edition), 10, 248-261.

Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis. / Balakrishnan, Rengasamy; Elangovan, Namasivayam; Mohankumar, Thangavel; Nataraj, Jegadeesan; Manivasagam, Thamilarasan; Justin Thenmozhi, Arokiasamy; Mohamed Essa, Musthafa; Akbar, Mohammed; Abdul Sattar Khan, Mohammed.

In: Frontiers in bioscience (Scholar edition), Vol. 10, 01.01.2018, p. 248-261.

Research output: Contribution to journalArticle

Balakrishnan, R, Elangovan, N, Mohankumar, T, Nataraj, J, Manivasagam, T, Justin Thenmozhi, A, Mohamed Essa, M, Akbar, M & Abdul Sattar Khan, M 2018, 'Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis', Frontiers in bioscience (Scholar edition), vol. 10, pp. 248-261.
Balakrishnan R, Elangovan N, Mohankumar T, Nataraj J, Manivasagam T, Justin Thenmozhi A et al. Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis. Frontiers in bioscience (Scholar edition). 2018 Jan 1;10:248-261.
Balakrishnan, Rengasamy ; Elangovan, Namasivayam ; Mohankumar, Thangavel ; Nataraj, Jegadeesan ; Manivasagam, Thamilarasan ; Justin Thenmozhi, Arokiasamy ; Mohamed Essa, Musthafa ; Akbar, Mohammed ; Abdul Sattar Khan, Mohammed. / Isolongifolene attenuates rotenone-induced mitochondrial dysfunction, oxidative stress and apoptosis. In: Frontiers in bioscience (Scholar edition). 2018 ; Vol. 10. pp. 248-261.
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