TY - JOUR
T1 - Isolation of (GA)n Microsatellite Sequences and Description of a Predicted MADS-box Sequence Isolated from Common Bean (Phaseolus vulgaris L.)
AU - Yaish, M. W.F.
AU - Pérez de la Vega, Marcelino
PY - 2003/9
Y1 - 2003/9
N2 - The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which represents the first MADS-box locus described to date in common bean, showed a very high similarity with other known MADS-box sequences and was grouped within the AGL2 subfamily cluster of the Arabidopsis MADS-box genes. The vicinity of microsatellites to some genes is also discussed.
AB - The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA)n microsatellites with n values equal or higher than six. These (GA)n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA)n microsatellite linked to a putative MADS-box gene was identified. This sequence, which represents the first MADS-box locus described to date in common bean, showed a very high similarity with other known MADS-box sequences and was grouped within the AGL2 subfamily cluster of the Arabidopsis MADS-box genes. The vicinity of microsatellites to some genes is also discussed.
KW - Genetic markers
KW - MADS-box
KW - Microsatellites
KW - Phaseolus vulgaris L.
KW - Transcriptional factor
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U2 - 10.1590/S1415-47572003000300019
DO - 10.1590/S1415-47572003000300019
M3 - Article
AN - SCOPUS:0142242097
SN - 1415-4757
VL - 26
SP - 337
EP - 342
JO - Genetics and Molecular Biology
JF - Genetics and Molecular Biology
IS - 3
ER -