Isolation of an elicitor from Rhizoctonia solani, the rice sheath blight pathogen which activates phenylpropanoid metabolism in suspension-cultured rice cells

R. Velazhahan, P. Vidhyasekaran

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A high molecular weight elicitor fraction has been isolated from the mycelial walls of Rhizoctonia solani, the rice sheath blight pathogen. It appears to be a glycoprotein. The elicitor preparation induced the activities of phenylalanine ammonia-lyase (PAL) and 4-coumarate: CoA ligase (4CL) in suspension-cultured rice cells and the maximum increase was observed within 12 h after elicitor treatment. Following increase of the first and last enzymes of phenylpropanoid pathway, phenolic content in the suspension-cultured rice cells increased to a maximum at 24 h after elicitor treatment. A co-ordinated increase in PAL and 4CL activities with a concomitant increase in phenolic content in cultured cells of rice due to an elicitor treatment is reported.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalZeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
Volume107
Issue number2
Publication statusPublished - Mar 2000

Fingerprint

phenylpropanoids
Thanatephorus cucumeris
blight
rice
metabolism
coumarate-CoA ligase
pathogens
phenylalanine ammonia-lyase
cells
cultured cells
glycoproteins
elicitors
molecular weight
enzymes

Keywords

  • 4-Coumarate
  • CoA ligase
  • Elicitor
  • Phenolics
  • Phenylalanine ammonia-lyase
  • Rhizoctonia solani
  • Rice cells

ASJC Scopus subject areas

  • Plant Science

Cite this

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abstract = "A high molecular weight elicitor fraction has been isolated from the mycelial walls of Rhizoctonia solani, the rice sheath blight pathogen. It appears to be a glycoprotein. The elicitor preparation induced the activities of phenylalanine ammonia-lyase (PAL) and 4-coumarate: CoA ligase (4CL) in suspension-cultured rice cells and the maximum increase was observed within 12 h after elicitor treatment. Following increase of the first and last enzymes of phenylpropanoid pathway, phenolic content in the suspension-cultured rice cells increased to a maximum at 24 h after elicitor treatment. A co-ordinated increase in PAL and 4CL activities with a concomitant increase in phenolic content in cultured cells of rice due to an elicitor treatment is reported.",
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