Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt

S. H.A. Hassan, R. N.N. Abskharon, S. M.F. Gad El-Rab, A. A.M. Shoreit

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Sixty six isolates of Pseudomonas spp. were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weight 27 Kb for nickel and lead. It was identified as Pseudomonas aeruginosa ASU 6a. Its minimal inhibitory concentration (MIC) for Cu2+, Co2+, Ni2+, Zn2+, Cr3+, Cd2+and Pb2+ were 6.3, 5.9, 6.8, 9.2, 5.8, 4.4, and 3.1 mM, respectively. Growth kinetics and the maximum adsorption capacities were determined under Ni2+ and Pb 2+ stress. The latter heavy metals induced potassium efflux and were used as indicator for plasma membrane permeabilization.

Original languageEnglish
Pages (from-to)168-176
Number of pages9
JournalJournal of Basic Microbiology
Volume48
Issue number3
DOIs
Publication statusPublished - Jun 2008
Externally publishedYes

Keywords

  • Heavy metal resistance
  • K efflux
  • Lipid peroxidation
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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