Isolation and identification of indigenous marine diatoms (Bacillariophyta) for biomass production in open raceway ponds

Ines Barkia, Fahad Zadjali, Nazamid Saari, Muta Harah Zakaria, Chun L. Li, Andrzej Witkowski, Lamya Al-Haj

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

For the successful, large-scale cultivation of microalgae, which for our work refers to diatoms, it is important to select the appropriate species for the right environment. Intensive research on the growth of different strains of microalgae from several regions worldwide is an ongoing effort. The aim of our research in this context was to select the best isolates of diatoms from different coastal sites in Oman, a region whose flora is poorly known, and identify these species using molecular (nuclear-encoded small subunit ribosomal RNA and chloroplast-encoded rbcL and psbC) and morphologic data. We aimed also to investigate and measure the growth rate, biomass production and fatty acid composition under ambient environmental conditions. Our results showed that in contrast to most of the research reported in the literature, the production of biomass from six isolates has been successfully carried out with reduced amounts of nutrients and without CO2 addition. Growth rates for species cultivated outdoors were high and ranged from 0.37 to 0.92 day-1. These findings are economically very promising as fast growth rates, associated with reduced operational costs, could remarkably improve the production efficiency and thereby justify their use as biomass feedstocks. Molecular data revealed that one isolate was Bellerochea malleus, four isolates represented an unidentified species of Bellerochea and the remaining strain studied represented an unidentified species of the genus Nitzschia. These six strains were rich in polyunsaturated fatty acids, with an average of 47% of the total fatty acids, confirming their potential use as aquaculture feed supplements.

Original languageEnglish
JournalAquaculture Research
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

raceways
Bellerochea
Bacillariophyceae
biomass production
diatom
pond
microalgae
biomass
fatty acid
aquaculture feeds
Nitzschia
Oman
feed supplements
feedstocks
polyunsaturated fatty acids
chloroplasts
flora
fatty acid composition
ribosomal RNA
fatty acids

Keywords

  • Biomass yield
  • Fatty acids
  • Marine diatoms
  • Microalgae
  • Molecular markers

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Isolation and identification of indigenous marine diatoms (Bacillariophyta) for biomass production in open raceway ponds. / Barkia, Ines; Zadjali, Fahad; Saari, Nazamid; Zakaria, Muta Harah; Li, Chun L.; Witkowski, Andrzej; Al-Haj, Lamya.

In: Aquaculture Research, 2017.

Research output: Contribution to journalArticle

@article{81f2f3a8cbdf4a4fbf939705cfcf2ef5,
title = "Isolation and identification of indigenous marine diatoms (Bacillariophyta) for biomass production in open raceway ponds",
abstract = "For the successful, large-scale cultivation of microalgae, which for our work refers to diatoms, it is important to select the appropriate species for the right environment. Intensive research on the growth of different strains of microalgae from several regions worldwide is an ongoing effort. The aim of our research in this context was to select the best isolates of diatoms from different coastal sites in Oman, a region whose flora is poorly known, and identify these species using molecular (nuclear-encoded small subunit ribosomal RNA and chloroplast-encoded rbcL and psbC) and morphologic data. We aimed also to investigate and measure the growth rate, biomass production and fatty acid composition under ambient environmental conditions. Our results showed that in contrast to most of the research reported in the literature, the production of biomass from six isolates has been successfully carried out with reduced amounts of nutrients and without CO2 addition. Growth rates for species cultivated outdoors were high and ranged from 0.37 to 0.92 day-1. These findings are economically very promising as fast growth rates, associated with reduced operational costs, could remarkably improve the production efficiency and thereby justify their use as biomass feedstocks. Molecular data revealed that one isolate was Bellerochea malleus, four isolates represented an unidentified species of Bellerochea and the remaining strain studied represented an unidentified species of the genus Nitzschia. These six strains were rich in polyunsaturated fatty acids, with an average of 47{\%} of the total fatty acids, confirming their potential use as aquaculture feed supplements.",
keywords = "Biomass yield, Fatty acids, Marine diatoms, Microalgae, Molecular markers",
author = "Ines Barkia and Fahad Zadjali and Nazamid Saari and Zakaria, {Muta Harah} and Li, {Chun L.} and Andrzej Witkowski and Lamya Al-Haj",
year = "2017",
doi = "10.1111/are.13539",
language = "English",
journal = "Aquaculture Research",
issn = "1355-557X",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Isolation and identification of indigenous marine diatoms (Bacillariophyta) for biomass production in open raceway ponds

AU - Barkia, Ines

AU - Zadjali, Fahad

AU - Saari, Nazamid

AU - Zakaria, Muta Harah

AU - Li, Chun L.

AU - Witkowski, Andrzej

AU - Al-Haj, Lamya

PY - 2017

Y1 - 2017

N2 - For the successful, large-scale cultivation of microalgae, which for our work refers to diatoms, it is important to select the appropriate species for the right environment. Intensive research on the growth of different strains of microalgae from several regions worldwide is an ongoing effort. The aim of our research in this context was to select the best isolates of diatoms from different coastal sites in Oman, a region whose flora is poorly known, and identify these species using molecular (nuclear-encoded small subunit ribosomal RNA and chloroplast-encoded rbcL and psbC) and morphologic data. We aimed also to investigate and measure the growth rate, biomass production and fatty acid composition under ambient environmental conditions. Our results showed that in contrast to most of the research reported in the literature, the production of biomass from six isolates has been successfully carried out with reduced amounts of nutrients and without CO2 addition. Growth rates for species cultivated outdoors were high and ranged from 0.37 to 0.92 day-1. These findings are economically very promising as fast growth rates, associated with reduced operational costs, could remarkably improve the production efficiency and thereby justify their use as biomass feedstocks. Molecular data revealed that one isolate was Bellerochea malleus, four isolates represented an unidentified species of Bellerochea and the remaining strain studied represented an unidentified species of the genus Nitzschia. These six strains were rich in polyunsaturated fatty acids, with an average of 47% of the total fatty acids, confirming their potential use as aquaculture feed supplements.

AB - For the successful, large-scale cultivation of microalgae, which for our work refers to diatoms, it is important to select the appropriate species for the right environment. Intensive research on the growth of different strains of microalgae from several regions worldwide is an ongoing effort. The aim of our research in this context was to select the best isolates of diatoms from different coastal sites in Oman, a region whose flora is poorly known, and identify these species using molecular (nuclear-encoded small subunit ribosomal RNA and chloroplast-encoded rbcL and psbC) and morphologic data. We aimed also to investigate and measure the growth rate, biomass production and fatty acid composition under ambient environmental conditions. Our results showed that in contrast to most of the research reported in the literature, the production of biomass from six isolates has been successfully carried out with reduced amounts of nutrients and without CO2 addition. Growth rates for species cultivated outdoors were high and ranged from 0.37 to 0.92 day-1. These findings are economically very promising as fast growth rates, associated with reduced operational costs, could remarkably improve the production efficiency and thereby justify their use as biomass feedstocks. Molecular data revealed that one isolate was Bellerochea malleus, four isolates represented an unidentified species of Bellerochea and the remaining strain studied represented an unidentified species of the genus Nitzschia. These six strains were rich in polyunsaturated fatty acids, with an average of 47% of the total fatty acids, confirming their potential use as aquaculture feed supplements.

KW - Biomass yield

KW - Fatty acids

KW - Marine diatoms

KW - Microalgae

KW - Molecular markers

UR - http://www.scopus.com/inward/record.url?scp=85032972721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032972721&partnerID=8YFLogxK

U2 - 10.1111/are.13539

DO - 10.1111/are.13539

M3 - Article

JO - Aquaculture Research

JF - Aquaculture Research

SN - 1355-557X

ER -