Isolation and characterization of Pseudomonas aeruginosa with antagonistic activity against Pythium aphanidermatum

A. H. Al-Hinai, A. M. Al-Sadi, S. N. Al-Bahry, A. S. Mothershaw, F. A. Al-Said, S. A. Al-Harthi, M. L. Deadman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A study was conducted to isolate and characterize bacterial strains from greenhouse soils with potential for suppression of Pythium damping-off of cucumber. Out of 100 bacterial isolates collected from greenhouse soils, 20 isolates were found to be antagonistic to Pythium aphanidermatum in in vitro studies. The size of the growth inhibition zone of P. aphanidermatum on potato dextrose agar varied from 4 to 9 mm, with an average of 6.2 mm. Identification of these bacterial isolates to the species level using API20NE as well as sequences of the 16S ribosomal RNA confirmed their identity as Pseudomonas aeruginosa. Bacterial isolates showed optimum growth at 30°C, whereas a temperature of 37°C favored bacterial antagonistic activity. Bacterial growth and antagonistic activity were not affected at high salinity levels (10 dS m-1) known to be stressful to cucumber production. This provides evidence for tolerance to salinity among the isolated bacteria. In vivo assays provided evidence for inhibition of Pythium damping-off at 28°C, with no effect on growth of cucumber seedlings. This is the first report of occurrence of Ps. aeruginosa with antagonistic activity against P. aphanidermatum in greenhouse soils in Oman.

Original languageEnglish
Pages (from-to)653-660
Number of pages8
JournalJournal of Plant Pathology
Volume92
Issue number3
Publication statusPublished - Nov 2010

Fingerprint

Pythium aphanidermatum
greenhouse soils
Pseudomonas aeruginosa
cucumbers
damping off
Pythium
salinity
Oman
microbial activity
in vitro studies
growth retardation
microbial growth
agar
ribosomal RNA
potatoes
glucose
seedlings
bacteria
assays
temperature

Keywords

  • Antagonism
  • Asparagine
  • Cucumis sativus
  • Metalaxyl
  • Salinity

ASJC Scopus subject areas

  • Plant Science

Cite this

Isolation and characterization of Pseudomonas aeruginosa with antagonistic activity against Pythium aphanidermatum. / Al-Hinai, A. H.; Al-Sadi, A. M.; Al-Bahry, S. N.; Mothershaw, A. S.; Al-Said, F. A.; Al-Harthi, S. A.; Deadman, M. L.

In: Journal of Plant Pathology, Vol. 92, No. 3, 11.2010, p. 653-660.

Research output: Contribution to journalArticle

Al-Hinai, A. H. ; Al-Sadi, A. M. ; Al-Bahry, S. N. ; Mothershaw, A. S. ; Al-Said, F. A. ; Al-Harthi, S. A. ; Deadman, M. L. / Isolation and characterization of Pseudomonas aeruginosa with antagonistic activity against Pythium aphanidermatum. In: Journal of Plant Pathology. 2010 ; Vol. 92, No. 3. pp. 653-660.
@article{cc577b38dcf341bf92307ad9467c1b1e,
title = "Isolation and characterization of Pseudomonas aeruginosa with antagonistic activity against Pythium aphanidermatum",
abstract = "A study was conducted to isolate and characterize bacterial strains from greenhouse soils with potential for suppression of Pythium damping-off of cucumber. Out of 100 bacterial isolates collected from greenhouse soils, 20 isolates were found to be antagonistic to Pythium aphanidermatum in in vitro studies. The size of the growth inhibition zone of P. aphanidermatum on potato dextrose agar varied from 4 to 9 mm, with an average of 6.2 mm. Identification of these bacterial isolates to the species level using API20NE as well as sequences of the 16S ribosomal RNA confirmed their identity as Pseudomonas aeruginosa. Bacterial isolates showed optimum growth at 30°C, whereas a temperature of 37°C favored bacterial antagonistic activity. Bacterial growth and antagonistic activity were not affected at high salinity levels (10 dS m-1) known to be stressful to cucumber production. This provides evidence for tolerance to salinity among the isolated bacteria. In vivo assays provided evidence for inhibition of Pythium damping-off at 28°C, with no effect on growth of cucumber seedlings. This is the first report of occurrence of Ps. aeruginosa with antagonistic activity against P. aphanidermatum in greenhouse soils in Oman.",
keywords = "Antagonism, Asparagine, Cucumis sativus, Metalaxyl, Salinity",
author = "Al-Hinai, {A. H.} and Al-Sadi, {A. M.} and Al-Bahry, {S. N.} and Mothershaw, {A. S.} and Al-Said, {F. A.} and Al-Harthi, {S. A.} and Deadman, {M. L.}",
year = "2010",
month = "11",
language = "English",
volume = "92",
pages = "653--660",
journal = "Journal of Plant Pathology",
issn = "1125-4653",
publisher = "Edizioni E.T.S.",
number = "3",

}

TY - JOUR

T1 - Isolation and characterization of Pseudomonas aeruginosa with antagonistic activity against Pythium aphanidermatum

AU - Al-Hinai, A. H.

AU - Al-Sadi, A. M.

AU - Al-Bahry, S. N.

AU - Mothershaw, A. S.

AU - Al-Said, F. A.

AU - Al-Harthi, S. A.

AU - Deadman, M. L.

PY - 2010/11

Y1 - 2010/11

N2 - A study was conducted to isolate and characterize bacterial strains from greenhouse soils with potential for suppression of Pythium damping-off of cucumber. Out of 100 bacterial isolates collected from greenhouse soils, 20 isolates were found to be antagonistic to Pythium aphanidermatum in in vitro studies. The size of the growth inhibition zone of P. aphanidermatum on potato dextrose agar varied from 4 to 9 mm, with an average of 6.2 mm. Identification of these bacterial isolates to the species level using API20NE as well as sequences of the 16S ribosomal RNA confirmed their identity as Pseudomonas aeruginosa. Bacterial isolates showed optimum growth at 30°C, whereas a temperature of 37°C favored bacterial antagonistic activity. Bacterial growth and antagonistic activity were not affected at high salinity levels (10 dS m-1) known to be stressful to cucumber production. This provides evidence for tolerance to salinity among the isolated bacteria. In vivo assays provided evidence for inhibition of Pythium damping-off at 28°C, with no effect on growth of cucumber seedlings. This is the first report of occurrence of Ps. aeruginosa with antagonistic activity against P. aphanidermatum in greenhouse soils in Oman.

AB - A study was conducted to isolate and characterize bacterial strains from greenhouse soils with potential for suppression of Pythium damping-off of cucumber. Out of 100 bacterial isolates collected from greenhouse soils, 20 isolates were found to be antagonistic to Pythium aphanidermatum in in vitro studies. The size of the growth inhibition zone of P. aphanidermatum on potato dextrose agar varied from 4 to 9 mm, with an average of 6.2 mm. Identification of these bacterial isolates to the species level using API20NE as well as sequences of the 16S ribosomal RNA confirmed their identity as Pseudomonas aeruginosa. Bacterial isolates showed optimum growth at 30°C, whereas a temperature of 37°C favored bacterial antagonistic activity. Bacterial growth and antagonistic activity were not affected at high salinity levels (10 dS m-1) known to be stressful to cucumber production. This provides evidence for tolerance to salinity among the isolated bacteria. In vivo assays provided evidence for inhibition of Pythium damping-off at 28°C, with no effect on growth of cucumber seedlings. This is the first report of occurrence of Ps. aeruginosa with antagonistic activity against P. aphanidermatum in greenhouse soils in Oman.

KW - Antagonism

KW - Asparagine

KW - Cucumis sativus

KW - Metalaxyl

KW - Salinity

UR - http://www.scopus.com/inward/record.url?scp=78650721925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650721925&partnerID=8YFLogxK

M3 - Article

VL - 92

SP - 653

EP - 660

JO - Journal of Plant Pathology

JF - Journal of Plant Pathology

SN - 1125-4653

IS - 3

ER -