Isolation and characterization of a new subtype of Borna disease virus

Norbert Nowotny, Jolanta Kolodziejek, Christian O. Jehle, Angelika Suchy, Peter Staeheli, Martin Schwemmle

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Borna disease virus (BDV), the causative agent of severe meningoencephalitis in a wide variety of animal species, has been considered to be genetically invariable and to form a single type within the genus Bornavirus of the family Bornaviridae. BDV infections are of particular interest, because for the first time a virus infection appears to be linked to human psychiatric disorders. We now describe a new subtype of BDV isolated from a horse which was euthanatized due to severe, incurable neurological disease. The nucleotide sequence of this new strain, named No/98, differs from the reference strains by more than 15%, and the subtype is difficult to detect by standard reverse transcriptase PCR protocols. The nucleotide exchanges of the novel BDV isolate have surprisingly little effect on the primary structures of most viral proteins, with the notable exception of the X protein (p10), which is only 81% identical to its counterpart in reference strains. Our data indicate that the genome of BDV is far more variable than previously assumed and that naturally occurring subtypes may escape detection by currently used diagnostic assays.

Original languageEnglish
Pages (from-to)5655-5658
Number of pages4
JournalJournal of Virology
Volume74
Issue number12
DOIs
Publication statusPublished - 2000

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Borna disease virus
Bornaviridae
Virus Diseases
Bornavirus
behavior disorders
Meningoencephalitis
meningoencephalitis
viral proteins
Viral Proteins
Reverse Transcriptase Polymerase Chain Reaction
infection
Horses
Psychiatry
Nucleotides
nucleotides
reverse transcriptase polymerase chain reaction
Genome
horses
nucleotide sequences
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Nowotny, N., Kolodziejek, J., Jehle, C. O., Suchy, A., Staeheli, P., & Schwemmle, M. (2000). Isolation and characterization of a new subtype of Borna disease virus. Journal of Virology, 74(12), 5655-5658. https://doi.org/10.1128/JVI.74.12.5655-5658.2000

Isolation and characterization of a new subtype of Borna disease virus. / Nowotny, Norbert; Kolodziejek, Jolanta; Jehle, Christian O.; Suchy, Angelika; Staeheli, Peter; Schwemmle, Martin.

In: Journal of Virology, Vol. 74, No. 12, 2000, p. 5655-5658.

Research output: Contribution to journalArticle

Nowotny, N, Kolodziejek, J, Jehle, CO, Suchy, A, Staeheli, P & Schwemmle, M 2000, 'Isolation and characterization of a new subtype of Borna disease virus', Journal of Virology, vol. 74, no. 12, pp. 5655-5658. https://doi.org/10.1128/JVI.74.12.5655-5658.2000
Nowotny, Norbert ; Kolodziejek, Jolanta ; Jehle, Christian O. ; Suchy, Angelika ; Staeheli, Peter ; Schwemmle, Martin. / Isolation and characterization of a new subtype of Borna disease virus. In: Journal of Virology. 2000 ; Vol. 74, No. 12. pp. 5655-5658.
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