Involvement of TRPV1-dependent and -independent components in the regulation of vagally induced contractions in the mouse esophagus

Ammar Boudaka, Jürgen Wörl, Takahiko Shiina, Winfried L. Neuhuber, Haruo Kobayashi, Yasutake Shimizu, Tadashi Takewaki

Research output: Contribution to journalArticle

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Abstract

Transient receptor potential ion channel of the vanilloid type 1 (TRPV1)-dependent pathway, consisting of capsaicin-sensitive tachykininergic primary afferent and myenteric nitrergic neurons, has been suggested to mediate the inhibitory effect of capsaicin on vagally mediated striated muscle contractions in the rat esophagus. In a recent study, similar but also different effects of capsaicin and piperine on TRPV1 were demonstrated. Therefore, this study aimed to compare the effects of these two drugs on vagally induced contractions in the mouse esophagus. Capsaicin and piperine inhibited vagally induced contractions of a thoracic esophageal segment in a concentration-dependent manner. Ruthenium red (10 μM; a non-selective blocker of transient receptor potential cation channels) and SB-366791 (10 μM; a novel selective antagonist of TRPV1) blocked the inhibitory effect of capsaicin but not that of piperine. Piperine inhibited the vagally mediated contractions in esophagi of adult mice neonatally injected with capsaicin, while capsaicin failed to do so. Desensitization of TRPV1 in the mouse esophagus by in vitro pretreatment with capsaicin failed to affect the inhibitory effect of piperine, whereas the piperine effect was cross-desensitized by capsaicin pretreatment in rat and hamster esophagi. Additionally, a tachykinin NK1 receptor antagonist, L-732,138 (1 μM), as well as a nitric oxide synthase inhibitor, NG-nitro-l-arginine methyl ester (l-NAME 200 μM), blocked the inhibitory effect of capsaicin but not that of piperine. Taken together, the results suggest that piperine inhibits the vagally mediated striated muscle contraction in the mouse esophagus through its action on a TRPV1-dependent pathway as well as a TRPV1-independent site.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalEuropean Journal of Pharmacology
Volume556
Issue number1-3
DOIs
Publication statusPublished - Feb 5 2007

Fingerprint

piperine
Capsaicin
Esophagus
Transient Receptor Potential Channels
Striated Muscle
Muscle Contraction
3,5-bis(trifluoromethyl)benzyl N-acetyltryptophan
Nitrergic Neurons
Tachykinin Receptors
Ruthenium Red
Ion Channels
Nitric Oxide Synthase
Cricetinae

Keywords

  • (Mouse)
  • (Rat)
  • Capsaicin
  • Esophageal motility
  • Esophagus
  • Piperine
  • Primary afferent neuron
  • Striated muscle
  • TRPV1
  • Vagus nerve

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Involvement of TRPV1-dependent and -independent components in the regulation of vagally induced contractions in the mouse esophagus. / Boudaka, Ammar; Wörl, Jürgen; Shiina, Takahiko; Neuhuber, Winfried L.; Kobayashi, Haruo; Shimizu, Yasutake; Takewaki, Tadashi.

In: European Journal of Pharmacology, Vol. 556, No. 1-3, 05.02.2007, p. 157-165.

Research output: Contribution to journalArticle

Boudaka, Ammar ; Wörl, Jürgen ; Shiina, Takahiko ; Neuhuber, Winfried L. ; Kobayashi, Haruo ; Shimizu, Yasutake ; Takewaki, Tadashi. / Involvement of TRPV1-dependent and -independent components in the regulation of vagally induced contractions in the mouse esophagus. In: European Journal of Pharmacology. 2007 ; Vol. 556, No. 1-3. pp. 157-165.
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