Investigation of the effect of UV irradiation on DNA damage

Comparison between skin cancer patients and normal volunteers

Mohamed J E M F Mabruk, Lim K. Toh, Miriam Murphy, Mary Leader, Elaine Kay, Gillian M. Murphy

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background Susceptibility to environmental carcinogenesis is the consequence of a complex interplay between intrinsic hereditary factors and actual exposure to potential carcinogenic agents. Exposure to sunlight is the primary etiological agent for basal cell carcinoma (BCC). Aim The aim of this study was to determine the effects of different ultraviolet (UV) doses on DNA damage in epidermal keratinocytes in vivo and to elucidate if patients with BCC are more susceptible to UV-induced DNA damage in comparison with normal healthy volunteers in response to solar simulator radiation (SSR). Materials and Methods Skin biopsies obtained post-UV irradiation from both normal healthy volunteers and BCC patients were analyzed for DNA damage, using immunohistochemical approach with TDM-2 antibody, which binds specifically to cyclobutane pyrimidine dimmers (CPDs). Results In both normal volunteers and BCC patients, the peak of CPD-positive cells occurred at 4.5 h post-SSR. There was a statistically significant difference in CPD positivity between BCC patients and normal volunteers, at time points (from 4.5 h to 48 h post-SSR). For a given dose of SSR based on each individual minimal erythema dose (MED), a greater number of CPD-positive cells could be shown. Conclusions This study has shown for the first time and in vivo in human volunteers that BCC patients are more susceptible to UV-induced DNA damage in comparison with normal healthy volunteers.

Original languageEnglish
Pages (from-to)760-765
Number of pages6
JournalJournal of Cutaneous Pathology
Volume36
Issue number7
DOIs
Publication statusPublished - Jul 2009

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Skin Neoplasms
DNA Damage
Basal Cell Carcinoma
Healthy Volunteers
Cyclobutanes
Radiation
Intrinsic Factor
Sunlight
Erythema
Keratinocytes
Volunteers
Carcinogenesis
Biopsy
Skin
pyrimidine
Antibodies

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Investigation of the effect of UV irradiation on DNA damage : Comparison between skin cancer patients and normal volunteers. / Mabruk, Mohamed J E M F; Toh, Lim K.; Murphy, Miriam; Leader, Mary; Kay, Elaine; Murphy, Gillian M.

In: Journal of Cutaneous Pathology, Vol. 36, No. 7, 07.2009, p. 760-765.

Research output: Contribution to journalArticle

Mabruk, Mohamed J E M F ; Toh, Lim K. ; Murphy, Miriam ; Leader, Mary ; Kay, Elaine ; Murphy, Gillian M. / Investigation of the effect of UV irradiation on DNA damage : Comparison between skin cancer patients and normal volunteers. In: Journal of Cutaneous Pathology. 2009 ; Vol. 36, No. 7. pp. 760-765.
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